DETERMINATION OF ANTIOXIDANT ACTIVITIES OF BUAS-BUAS LEAVES (Premna serratifolia L.) USING DPPH (2,2-diphenyl-1-picrylhydrazyl) METHOD

https://doi.org/10.22146/tradmedj.17292

Isnindar Isnindar(1*), Subagus Wahyuono(2), Sitarina Widyarini(3), Yuswanto Yuswanto(4)

(1) Faculty of Medicine, Study Program of Pharmacy, Unversitas of Tanjung Pura
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Animal Medicine, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Antioxidant is a substance that in small concentrations can significantly inhibit or prevent oxidation on the substrate. Buas-buas (Premna serratifolia L.) is one of the plants that have antioxidant effect. The antioxidant activities of the wasbenzen and ethanol extracts of buas-buas leaves were determined by DPPH method using spectrophotometer UV-Vis. Data resulted from the analysis of antioxidant activity (IC50) is 532.24 μg/mL of wasbenzen extract, 24.40 μg/mL of ethanol extract, and 1.83 μg/mL of vitamin C.


Keywords


Buas-buas leaves; Premna serratifolia L; wasbenzen extract; ethanol extract; antioxidant activity; DPPH method

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References

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DOI: https://doi.org/10.22146/tradmedj.17292

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