ANTIOXIDANT AND ANTIBACTERIAL CAPACITY OF LOLOH SEMBUNG (Blumea balsamifera) BASED ON EXTRACTION METHOD

https://doi.org/10.22146/tradmedj.17318

IGA. Wita Kusumawati(1*), IBA. Yogeswara(2)

(1) Faculty of Health Science and Technology, Universitas Dhyana, Bali
(2) Daun sembukan, fraksi etil asetat, larvasida, Aedes aegypti, Anopheles
(*) Corresponding Author

Abstract


Loloh sembung (Blumea balsamifera) is a traditional herbal drink which of the extraction methods can be done by boiling and brewing. Loloh sembung was prepared from fresh and dried leaves. Loloh sembung extracted by different methods producing phenolic content, tannin content, antioxidant capacity, are different. Dried leaves were extracted by brewing have high content of total phenolic was at 13.15±0.11 mg GAE/g sample, while dried leaves were extracted by boiling have high content of tannin and antioxidant capacity were at 1.65±0.01 mg TAE/g sample and 5.55±0.01 mg GAE/g sample respectiveliy. Both of fresh and dried leaves were extracted by boiling and brewing were not show inhibition against Escherichia coli and Staphylococcus aureus bacteria.

Keywords


loloh sembung, total phenolic content, tannin content, antioxidant capacity, antibacterial



References

Ammar, I., Monia E. and Hamadi A. 2015. Phenolic content and antioxidant activity of cactus (Opuntiaficus-indica L.) flowers are modified according to the extraction method. Industrial Crops and Products 64 : 97-104.

Ali, D.M.H., K.C. Wong., and P.K. Lim. 2005. Flavonoids from Blumea balsamifera. Fitoterapia 76 : 128–130.

Aminul, Shawkat Md. Islam., Kh Tanvir Ahmed., Mohammad Kawsar Manik., Md. Arif Wahid., and Chowdhury Shafayat Ibne Kamal. 2013. A comparative study of the antioxidant, antimicrobial, cytotoxic and thrombolytic potential of the fruits and leaves of Spondias dulcis. Asian Pac J Trop Biomed 3(9): 682-691.

Bauer, A.W., W. M. M. Kirby., J. C. Sherris, and M. Turck. 1966. Antibiotic Susceptibility Testing by a Standardized Single Disk Method. American Journal of Clinical Pathology, 45:493–496.

Benayad, Z., Cristina M-V., Juana F., Carmen G-C., and Nour, E.E. 2014. Phenolic composition, antioxidant and anti-inflammatory activities of extracts from Moroccan Opuntia ficus-indica flowers obtained by different extraction methods. Industrial crops and products 62 : 412-420.

Chirinos, R., Rogez, H., Campos, D., Pedreschi, R., and Larondelle, Y. 2007. Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropae-olum tuberosum Ruiz and Pavon) tubers. Separ. Purif. Technol. 55 :217-225.

Dai, Jin and Russell J. Mumper. 2010. Review Plant Phenolics : Extraction, Analysis and Their Antioxidant dan Anticancer Properties. Molecules 15 : 7313-7352.

Gilbert, A., Tadjoua T. H., Yousseu N. W., Sama F. L., Kuiate J-R., and Kamanyi A. 2014. Antidiarrhoeal and antibacterial activity of aqueous and methanolic leaves extracts of Dissotis thollonii Cogn. (Melastomataceae). Asian Pac J Trop Biomed 4 (Suppl 2): S672-S678

Hanani, Endang., Abdul Mun’im and Ryany Sekanni. 2005. Identifikasi Senyawa Antioksidan dalam Spons Callyspongia Sp dari Kepulauan Seribu. Majalah Ilmu Kefarmasian. Vol. III, No. 3. 127-133.

Mursito, B. 2002. Ramuan Tradisional untuk Pengobatan Jantung. Penerbit Swadaya. Jakarta.

Nathalie, Liesbeth Leurs. 2009. Medicinal, Aromatic and Cosmetic (MAC) Plants for Community Health and Bio-Cultural Diversity Conservation in Bali, Indonesia. L.N. Leurs page : 124-127.

Norikura, T., Kojima, Y.A. Shimizu, M., Huang, X. Xu, S., Kametani, S., Rho, S.N., Kennedy, D.O., and Matsui, Y.I. 2008. Anticancer activities and mechanisms of Blumea balsamifera extract in hepatocellular carcinoma cells. Am J Chin Med. 36(2):411-24.

Ongsakul, M., Jindarat, A., and Rojanaworarit, C. 2009. Antibacterial effect of crude alcoholic and aqueous extracts of six medical plant against Staphylococcus aureus and Escherichia coli. J. Health Res. 23: 153-156.

Sakee, U,. Maneerat S., Cushnie TP., De-Eknamkul W. 2011. Antimicrobial activity of Blumea balsamifera (Lin.) DC. extracts and essential oil. Nat Prod Res. 25(19):1849-56.

Sartinah, A., Puji A., and Subagus W. 2010. Isolasi dan indentifikasi senyawa antibakteri dari daun petai cina (Leucaena leucocephala (Lam.) De Wit). Majalah Obat Tradisional 15 : 146-152.

Suhardi. 1997. Analisis Senyawa Polifenol Produk Buah-buahan dan Sayuran Vol.3. Yogyakarta. Laboratorium Kimia-Biokimia Pengolahan Fakultas teknologi Pertanian Universitas Gajah Mada.

Sulaiman, S.F and Kheng L. O. 2012. Antioxidant and anti food-borne bacterial activities of extracts from leaf and different fruit parts of Myristica fragrans Houtt. Food Control 25 : 533-536.

Suweta, I Made. 2013. Ecolinguistics Approach in Preservation Rare Plants Growing in Bali. International Journal of Linguistics, Vol. 5 No. 1 : 283-295.

Szydłowska-Czerniak, Aleksandra., Agnieszka Tułodziecka., and Edward Szłyk. 2012. Determination of Antioxidant Capacity of Unprocessed and Processed Food Products by Spectrophotometric Methods. Food Anal. Methods 5:807–813.

Tan, Y. P., and Eric W. C.C. 2014. Antioxidant, antityrosinase and antibacterial properties of fresh and processed leaves of Anacardium occidentale and Piper betle. Food Bioscience 6 : 17-23.

Tsai, Y.L., Chiou, S.Y., Chan, K.C., Sung, J.M., and Lin, S.D. 2012. Cafferic acid derivates, total phenols, antioxidant and antimutagenic activities of Echinacea purpurea flower extracts. LWT-Food Sci. Technol 46 : 169-176

Wang, L., and Weller, C.L. 2006. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Technol. 17, 300-312.

Wang, A. Y., Zhou, M. Y., and Lin, W. C. 2011. Antioxidative and anti-inflammatory properties of Citrus sulcate extracts. Food Chem. 124 : 958-963.

Wita, I.G.A., I Pt Darmawijaya., and I.B.A Yogeswara. 2014. Potensi antioksidan loloh tempuyung (Sonchus arvensis L.) sebagai minuman fungsional. Prosiding Seminar Nasional Biologi FMIPA UNHI.



DOI: https://doi.org/10.22146/tradmedj.17318

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