DIOSGENIN DETERMINATION OF Costus speciosus WATER EXTRACT USING HPLC METHOD

https://doi.org/10.22146/tradmedj.24171

Hari Susanti(1*), Subagus Wahyuono(2), Ratna Asmah Susidarti(3), Ika Puspita Sari(4)

(1) Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Costus speciosus has been reported to have biological activity among antidiabetic, antihyperlipidemia, antioxidants. One of the chemical constituents that is responsible for some of the biological activity is diosgenin. This study aimed to determine diosgenin content of Costus speciosus water extract (CS). Water extract of CS was obtained with infundation method. The extract was dried using freeze dryer. The assay of diosgenin was performed by HPLC method. HPLC system used: LiChrospher® 100 RP-18 endcapped (5 µm) column length 25cm, colimn id 4 mm as stationary phase, acetonitrile-water (9: 1 v/v) as mobile phase, flow rate 2mL/min and UV detection at 205 nm. HPLC method developed was linear in the range 20-200 μg mL with R = 0.9999 ; slope = 6423.7; and intercept = -4280.5; LOD = 2.14 ug/mL and LOQ = 6.50 mg/mL; and recovery 100.63 %. Diosgenin levels in CS was 0.279 %. The developed HPLC method is relatively simple, rapid, sensitive, and accurate to determine the content of diosgenin in water extracts of Costus speciosus.

Keywords


water extract, Costus speciosus, HPLC, diosgenin



References

Abdelkarim A.S., Ali, B., Ali M., 2015, HPTLC determination of bioactive diosgenin for Sudanese and Indian fenugreek (Trigonellafoenum-graecum L.) seeds, International Journal of Advances in Pharmacy Medicine Bioallied Sciences , Vol 2, Issue 3:165-168.

Adnan and Pagarra, B., 2000, Effect of Rhizome Extract from Pacing (Costus speciosus, J.E Smith) on spermatogenesis mice (Mus musculus) ICR male, Research Report, Department of Biological Science, State University of Makassar.

Ahuja, S., Dong, M.W., 2005. Handbook of Pharmaceutical Analysis by HPLC, New York : Elsevier Academic Press. 6: 201-203.

Amir, M. , Mujeeb M., Sabih , A., Ahmad, S., Ahmad, A., Siddiqui, WA., 2010, Validation of HPTLC Method for Estimation of Diosgenin in Callus and Rhizome of Dioscorea deltoidea, Planta Medica; 76 :35.

Anonim, 2008, Farmakope Herbal edisi I, Departemen Kesehatan RI, Jakarta.

Anonim, 2009. The United State Pharmacopoeia : 32th Edition. USA : Pharmacopoieal Convention.

AOAC, 2012. Official Methods of Analysis 2012 : Guidelines for Standard Method Performance Requirements. Appendix F, . 9.

Chapagain , B., and Wiesman, Z., 2005, Variation in diosgenin level in seed kernels among different provenances of Balanites aegyptiaca Del (Zygophyllaceae) and its correlationwith oil content. African J. Biotech. Vol. 4 (11): 1209-1213.

Devi dan Urooj, 2010, Nutrient Profile and antioxidant component of Costus speciosus, Natural Product Resources, 1(1): 116-118

Eliza J., Daisy, P., Ignacimuthu, S., Duaripadiyan, V, 2009, Antidiabetic and Antilipidemic Effect of Eremanthin from Costus speciosus (koen)Sm. In STZ-induced diabetic rat, Chemico-Biological Interaction , 182 : 67-72.

Eliza J., Daisy, P., Ignacimuthu, S., 2010, Antioxidant activity of costunolide and eremanthin isolated from Costus speciosus (Koen ex. Retz) Sm., Chemico-Biological Interaction, 188: 467-472.

Ghosh S, More P, Derle A, Patil AB, Markad P, et al.. 2014, Diosgenin from Dioscorea bulbifera: Novel Hit for Treatment of Type II Diabetes Mellitus with Inhibitory Activity against a-Amylase and a-Glucosidase. PLoS ONE 9(9): e106039 open access.

Joshi, B.N., Munat,H., Harkidar, M., Kulkarni, A.A., 2013,Orally Active Hypoglygemic protein from Costus igneus NE Br: An in vitro and in vivo Study, Biochemical and Biophysical Research Communication, 436:278-282.

Li, P., Mou Y, Lu, S., Sun , W., , Lou, J.,, Yin, C., and Zhou,L., 2012, Paper Quantitative determination of diosgenin in Dioscorea zingiberensis cell cultures by microplatespectrophotometry and high performance liquid chromatography, Af. J.Pharmacy Pharm. Vol. 6(15):1186 – 1193.

Qiao CF1, Li QW, Dong H, Xu LS, Wang ZT, 2002, Studies on Chemical Constituents of Two Plants from Costus, Zhongguo zhong Yao Zhi, 27(2):123-5 .

Selim, S. dan Al Jouni, S., 2015, Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm. International Society for Complementary Medicine Research 15:301, DOI: 10.1186/s12906-015-0836-8, open access

Snyder, L.R., Kirkland, J.J., Dolan J.W., 2010. Introduction to Modern Liquid Chromatography, 3th Ed., USA:John Wiley & Sons, Inc. Publication.

Son, IS.,,Kim, J.H., Sohn, H.Y., Son, K.H., 2007, Antioxidative and Hypolipidemic Effect of Diosgenin, a Steroidal Saponin of Yam (Dioscorea spp) on High-Cholesterol Fed Rats, Biosci. Biotechnol. Biochem. 71 (12) : 3063-3071.

Susanti H, 2015, Potensi Pacing Sebagai antihiperkolesterol, Laporan Penelitian, UAD, Yogyakarta.



DOI: https://doi.org/10.22146/tradmedj.24171

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