FORMULATION OF NANOEMULSION MOUTHWASH COMBINATION OF LEMONGRASS OIL (Cymbopogon citratus) AND KAFFIR LIME OIL (Citrus hystrix) FOR ANTICANDIDIASIS AGAINST Candida albicans ATCC 10231

https://doi.org/10.22146/tradmedj.24255

Meta Juniatik(1*), Khoirunnissa Hida(2), Fransisca Priskaningtyas Wulandari(3), Nurul Pangestuti(4), Ni'matul Munawaroh(5), Ronny Martien(6), Sylvia Utami Tunjung Pratiwi(7)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(5) Faculty of Pharmacy, Universitas Gadjah Mada
(6) Faculty of Pharmacy, Universitas Gadjah Mada
(7) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Candidiasis is a fungal infection disease that still become health problem in Indonesia, especially oral candidiasis that largerly caused by Candida albicans abnormal growth on oral cavity. Lemongrass oil and kaffir lime oil have been proven to have antifungal activity against Candida albicans. The composition of limonen, α-terpineol, sitronelil acetate, terpineol, β-pinene, on kaffir lime oil and citronelal on lemongrass oil make the combination of both oils have the potential as an alternative theraphy for oral candidiasis. It formulates in the form of nanoemulsion mouthwash. Nanoemulsion is chosen because it has some advantages like clear, stable, and increasing the antifungal activity of lemongrass oil and kaffir lime oil. The study begins with plants determination, essential oils charecterization tests (solubility, refractative index, and specific gravity), and essential oil content analysis by GC-MS. Nanoemulision formulates by water titration method. Optimization of oil mix and VCO do to get a clear and stable nanoemulsion. The best formula is characterized (transmittance, particle size, viscosity, zeta potential, and stability test), then analyze the essential oil chemical compounds in the optimum formula and test the inhibitory effect of the formula by microdilution method. Inhibiton data analysis use One Way ANOVA. Based on the results of the study, 0.4 % oil mix, 3.6 % VCO, 17.3 % tween 80, 8.7 % PEG 400 dan 70 % water is the best formula that can form a nanoemulsion system with an average particle size 21,4 nm, low viscosity, low zeta potential and stable during freez-thaw storage. Nanoemulsion significantly has inhibitory effect more effective than positive control.

Keywords


nanoemulsion; lemongrass oil; kaffir lime oil; Candida albicans; antifungal

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References

ASTM, 1985, Zeta Potential of Colloids in Water and Waste Water. American Society for Testing and Materials. pp 4187–4182.

Auburn, O.S., Simonnet, J.T. and L’Alloret, F. 2004. Nanoemulsions: A New Vehicle for Skincare Products. Advances In Colloid And Interface Science. 108 –109: 145–149. Badan Standar Nasional, 1995, SNI No.06-3953,

http://sisni.bsn.go.id/index.php?/sni_main/sni/detail_sni/4386, accessed 26 april 2016. Baser, K.H.C. and Buchbauer, G. 2010. Handbook of Essential Oils Science, Technology, and Applications. CRC Press. pp 921.

Bawalan, D.D. dan Chapman, K.R. 2006. Virgin Coconut Oil: Production Manual dor Micro and Village Scale Processing. Food and Agriculture Organization Regional Office for Asia and the Pacific Bangkok. pp 12-16.

Bird, R. B., Stewart, W. E. and Lightfood, E. N. 1960. Transport Phenomena. New York. John willey & sons, Inc.

Dalleau, S., Cateau, E., Berg`es, T., Berjeaud, J.M. and Imbert, C. 2008. In Vitro Activity of Terpenes Against Candida Biofilms. International Journal of Antimicrobial Agents. 31: 572–576.

Dignani, M.C., Solamkin, J.S. and Anaissie, E.J. 2009, Candida, Clinical Mycology 2nd Ed. 8: 197-230.

Dovigo, L.N., , Pavarina, A.C., Carmello, J.C., , Machado, A.L., Brunetti, I.L., Bagnato, V.S., 2011, Susceptibility of Clinical Isolates of Candida to Photodynamic Effects of Curcumin, Lasers in Surgery and Medicine, 43 (9):927-34.

Graves, S.M. 2008. The Formation, Optical Properties, and Structure of Nanoemulsions. Thesis. Los Angeles, University of California. Greenberg. M.S. 2003. Burket’s Oral Medicine. 10th Ed. Hamilton Ontario Bc Decker Inc. pp 94 – 98.

Gupta, P.K., Prandit, J.K., Kumar, A., Swaroop, P. and Gupta, S. 2010. Pharmaceutical Nanotechnologypharma Novel Nanoemulsion High Energy Emulsification Preparation, Evaluation, and Application. The Pharma Research Journal. 3: 117-138.

Harahap, M. 2000. Ilmu Penyakit Kulit. 1st Ed. Jakarta. Hipokrates. pp. 109-15. Jain, K., Kumar, R.S., Sood, S., and Gowthamarajan, K. 2013. Enhanced Oral Bioavailability of Atorvastatin Via Oil in Water Nanoemulsion Using Aqueous Titration Method. Journal of Pharmaceutical Sciences and Research. 5: 18- 25.

Jamil, K. F. 2014. Profil Kadar CD4 Terhadap Infeksi Oportunistik pada Penderita Human Immunodeficiency Virus / Acquired Immunodeficiency Syndrome (HIV/AIDS) Di RSUD dr. Zainoel Abidin Banda Aceh, Jurnal Kedokteran Syiah Kuala. 14: 2.

Karima, N. 2015. Formulasi SNEDDS (Self-Nanoemulsifying Drug Delivery System) Ekstrak Etanolik Buah Mengkudu (Morinda citrifolia L.) Menggunakan Minyak Nabati, Thesis. Yogyakarta. Universitas Gadjah Mada.

Kurnia, F. A. 2015. Optimasi Formula Nanoemulsi untuk Formulasi Nanoemulgel Ketoprofen Menggunakan Sunflower Oil, Tween 80-Propilen Glikol, dan Air. Thesis. Yogyakarta. Universitas Gadjah Mada.

Kusuma, A. L. 2014 Hubungan Kadar CD4 Dengan Kejadian Kandidiasis Oral Pada Penderita HIV/AIDS Di RSUD Moewardi Surakarta. Thesis. Surakarta. Universitas Muhammadiyah, Lalwani, J.T., Thakkar, V.T. and Patel, H.V. 2013. Enhancement of Solubility and Oral Bioavaibility of Ezetimibe by A Novel Solid Self Nano Emulsifying Drug Delivery System (SNEDDS). International Journal of Pharmacy and Pharmaceutical Science. 5(3): 513-522.

Lima, I.O., Oliveira, R. D. A. G., Lima, E. O., Souza, E. L., Farias, N. P., Navarro, D. F. and Farmacêuticas, R. B. C. 2005. Inhibitory Effect of Some Phytochemicals in The Growth of Yeasts Potentially Causing Opportunistic Infections. Brazilian Journal of Pharmaceutical Sciences. 4: 2.

Mason, T.G., Wilking, J.N., Meleson, K., Chang, C.B. and Graves, S.M. 2006. Nanoemulsions: Formation, Structure, and Physical Properties. J. Phys: Condens. 18: 635–666.

Omran, S.M., Moodi, M.A., Amiri, S.M.B.N., Mosavi, S.J., Saeed, S.A.M.G.M., Shiade, S.M.J., Kheradi, E., Salehi, M. 2011. The Effects of Limonene and Orange Peel Extracts on Some Spoilage Fungi, International Journal of Molecular and Clinical Microbiology. 1: 82-86.

Pinto, E., Gonçalves, M.J., Oliveira, P., Coelho, J., Cavaleiro, C. and Salgueiro, L. 2014. Activity of Thymus Caespititius Essential Oil and A-Terpineol Against Yeasts and Filamentous Fungi. Industrial Crops and Products, 62: 107–112.

Prajapati H.N., Dalrymple D.M., Serajuddin A.T.M., 2011, A Comparative Evaluation of Mono-, Di- and Triglyceride of Medium Chain Fatty Acids by Lipid/Surfactant/Water Phase Diagram, Solubility Determination and Dispersion Testing for Application in Pharmaceutical Dosage Form Development, Pharmaceutical Research.

Prasart, Foo-trakul and Chonlada, Watchiradatsatiean, 2014, Development of Anti-Dandruff Shampoo from Kaffir Lime which is the By-Product of Food Industry, Kasetsart J. (Nat. Sci.) 39 : 725 – 729.

Quave, C.L., Plano, L.R.W., Pantuso, T., & Bennet, B.C. 2008. Effect of Extract from Italian Medicinal Plant on Planktonic Growth, Biofilm Formation and Adherence of Methicillin Resistant Staphyllococcus aureus. Journal Ethnopharmacol. 118 : 418-428.

Rowe, C.R., Sheskey, J.P. and Weller, J.P. 2009. Handbook of Pharmaceutical Excipients. 6th Ed. American Pharmaceutical Association, London, Chicago.

Ruckmani, R and Lakshmi, T. 2013. Lemon Grass Oil For Improvement Of Oral Health. Thesis. Nadu. Saveetha Dental College and Hospitals.

Serano, I. O., Oliu, G. O. and Belloso, O. M. 2014. Nanoemulsion Based Delvery Systems to Improve Functional of Lipophilic Components. Front Nutr. 1: 24.

Sharma, N., Bansal, M., Visht, S., Kulkarni, G.T. and Sharma, P.K.. 2010. Nanoemulsion: A New Concept of Delivery System, Department of Pharmaceutical Technology Meerut Inst of Engg. and Tech. pp 2-6.

Simunkova, H., Pessenda-Garcia, P., Wosik, J., Angerer, P., Kronberger, H. and Nauer, G. E. 2009. The Fundamentals of Nano and Submicro Scaled Ceramic Particles Incorporation into Electrodeposited Nickel Layers: Zeta Potential Measurements. Surface and Coatings Technology. 203: 1806–1814.

Singh, D., Kumar, T.R.S., Gupta, I.K. and Chaturvedi, P. 2012. Antimicrobial Activity of Some Promising Plant Oils, Molecules and Formulations, Indian Journal of Experimental Biology. 50: 714-717.

Sinko, P.J. 2006. Martin’s Physical Pharmacy and Pharmaceutical Science. 5th Ed. Lippincott Williams & Wilkins. Philadelphia. pp 466-467.

Siregar, K.T.T., Tamba, T., Perangin-angin, B., 2013, Viskosimeter Digital Menggunakan Water Flow Sensor G1/2 Berbasis Mikrokontroller 8535, Saintika, 4:1.

Siregar, R.S. 2002. Penyakit Jamur Kuli. 2th. EGC. Jakarta. Taweechaisupapong, S., Aieamsaard, J., Chitropas, P. and Khunkitti, W. 2012 Inhibitory Effect of Lemongrass Oil and Its Majjor Constituents on Candida Biofilm and Germ Tube Formation. South African Journal of Botany. 81: 905-102.

Vior, M.C.G., Monteagudo, E., Dicelio, L.E. and Awruch, A. 2011. A Comparative Study of A Novel Lipophilic Phthalocyanine Incorporated into Nanoemulsion Formulations: Photophysics, Size, Solubility and Thermodynamic Stabilit. Dyes And Pigments. 91: 208-214.

Wahyuningsih, I. and Putranti, W. 2015. Optimasi Perbandingan Tween 80 dan Polietilenglikol 400 pada Formula Self Nanoemulsifying Drug Delivery System (SNEDDS) Minyak Biji Jinten Hitam. Pharmacy. 2: 223-241.

Widyaningrum, S.A., 2015, Formulasi Dan Uji Aktivitas Antimikroba Nanoemulsi Minyak Manis-Jangan (Cinnamomum burmanni Nees ex. BI.) terhadap Pseudomonas aeruginosa NCTC 12924 dan Staphylococcus aureus ATCC 29213, Thesis, Fakultas Farmasi, Universias Gadjah Mada, Yogyakarta



DOI: https://doi.org/10.22146/tradmedj.24255

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