Eris Septiana(1*), Partomuan Simanjuntak(2)

(1) Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Cibinong, West Java
(2) Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Cibinong, West Java Faculty of Pharmacy, Pancasila University, Srengseng Sawah, Jakarta
(*) Corresponding Author


Antioxidant is an interesting topic due to their capability to inhibit free radical and prevent damage because of oxidative processes. Endophyt fungi is one of antioxidant compound resources in nature. The low yield to gain antioxidant compound from fungi challenges to look for the composition of media and optimalization of growth conditions. This research aimed to know the effect of medium condition in different carbon and nitrogen sources as well as initial pH towards antioxidant activity of endophyt fungi Bo.Ci.Cl.A3. Shaker fermentation was used on 120 rpm at room temperature for 14 days. The carbon sources were glucose, sucrose, and starch and nitrogen sources were NaNO3, NH4NO3, and yeast extract with initial pH at 5, 7, and 9. Ethyl acetate was used as extractor. The results showed that endophyt fungi can produce secondary metabolite as antioxidant at all variation of fermented media. The nitrogen source of yeast extract could increase antioxidant activity of endophyt fungi Bo.Ci.Cl.A3, while other sources such as nitrogen source, carbon sources, and different initial pH on the basal medium that were used did not give increasing antioxidant activity. The conclusion of this research was the substitution of nitrogen source with yeast extract (3 g/L) on the basal medium Czapek Dox’s Broth could increase antioxidant activity of endophyt fungi Bo.Ci.Cl.A3.


antioxidant; DPPH; endophytic fungi; media variation


Abo-Elmag, H.I. 2014. Evaluation and optimization of antioxidant potentiality of Chaetomium madrasenseAUMC 9376. J. Genet. Engineer. Biotechnol. 12: 21-26.

Arora, D.S. and Chandra, P. 2010. Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions. Braz. J. Microbiol. 41: 765-777.

Bhattacharyya, P.N. and Jha,K.D. 2011. Optimization of cultural condition affecting growth and improved bioactive metabolite production by subsurface Aspergillus strain TSF-146. Int. J. Appl. Biol. Pharm. Technol. 2: 133-143.

Blois, M.S. 1958. Antioxidant determination by the use of stable free radicals. Nature. 181: 1199-2000.

Chandra, P. and Arora, D.S. 2014. Antioxidant potential of two strains of Aspergillus wentii isolated from soil of different areas of Punjab, India and its optimization using different statistical approaches. J. Microbiol. Biotech. Res. 4: 31-41.

Fox, E.M. and Howlett, J.B. 2008. Secondary metabolism: regulation and role in fungal biology. Curr. Opin. Microbiol. 11: 1-7.

Gazi, M.R., Kanda, K. and Kato, F. 2004. Optimization of various cultural conditions on growth and antioxidant activity generation by Saccharomyces cerevisiae IFO 2373. J. Biol. Sci. 4: 224-228.

Isnindar, Wahyuono, S., Widyarini, S. and Yuswanto. 2016. Determination of antioxidant activities of buas-buas leaves (Premna serratifolia L.) using dpph (2,2-diphenyl-1-picrylhydrazyl) method. Trad. Med. J. 21: 111-115.

Miao, L., Kwong, T.F.N. and Qian, P.Y. 2006. Effect of culture condition on mycelial growth, antibacterial activity, and metabolite profiles of the marine-derived fungus Arthrinium c.f. saccharicola. Appl. Microbiol. Biotechnol. 72: 1063-1073.

Miller, M.J., Diplock, A.T. and Rice-Evan, C.A. 1995. Evaluation of the total antioxidant activity as a marker of the deterioration of apple juice on storage. J. Agric. Food. Chem. 43: 1794-1801.

Moon, B.S., Ryoo, I.J., Yun, B.S., Bae, K.S., Lee, K.D., Yoo, I.D. and Kim, J.P. 2006. Glyscavins A,B and C, new phenolic glycoside antioxidant produced by a fungus Mycelia sterilia F020054. J. Antibiot. 59: 735-739.

Nogurira, M.A., Diaz, G., Andriali, W., Faiconi, A.F. and Stangarlin, S.R. 2006. Secondary metabolism from Diplodia maydis and Sclerotium rolfsi with antibiotic activity. Braz. J. Microbiol. 37: 14-16.

Radulovic, N., Stankov-Jovanovic, V., Stojanovic, G., Smelcerovic, A., Spiteller, M. and Asakawa, Y. 2007. Screening of in vitro antimicrobial and antioxidant activity of nine Hypericum species from the Balkans. Food. Chem. 103: 15-21.

Rios, M.F., Pajan, C.M.G., Galan, R.H., Sanchez, A.J.M. and Callado, I.G. 2006. Sythesis and free radical scavenging activity of a novel metabolite from the fungus Colletrotichum gloeosporioides. Bioorg. Med. Chem. Lett. 16: 5836-5839.

Septiana, E. 2014. Keragaman cendawan endofit asal tanaman kunyit (Curcuma longa) dan aktivitas penghambatannya terhadap pembentukan histamin, Thesis, Bogor, ipb.

Septiana, E. and Simanjuntak, P. 2016. Aktivitas penghambatan bakteri pembentuk histamine dan antioksidan kapang endofit kunyit sebagai pengawet alami. Biopropal Industri. 7: 1-8.

Tiwari, V., Shanker, R., Srivastava, J. and Vanker, P.S. 2006. Change in antioxidant activity of spices-turmeric and ginger on heat treatment. Electron. J. Environ. Agric. Food. Chem. 5: 1313-1317.

Yen, G. and Chang, Y. 1999. Medium optimization for the production of antioxidants from A candidus. J. Food. Prot. 62: 657-661.

DOI: https://doi.org/10.22146/tradmedj.24308

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