Identification of ɤ-Aminobutyric Acid (GABA) in Loloh as A Potential Drink as Antihipertension

I Gusti Ayu Wita Kusumawati(1*), Ni Made Indri Sugiantari(2), I Gede Ariyasa(3), Ida Bagus Agung Yogeswara(4)

(1) Program of Nutrition Study, Faculty of Health, Science, and Technology, University of Dhyana Pura, Bali
(2) Program of Nutrition Study, Faculty of Health, Science, and Technology, University of Dhyana Pura, Bali
(3) Program of Nutrition Study, Faculty of Health, Science, and Technology, University of Dhyana Pura, Bali
(4) 1) Program of Nutrition Study, Faculty of Health, Science, and Technology, University of Dhyana Pura, Bali 2) Department of Food Science and Technology, University of Natural Resources and Applied Life Sciences Bodenkultur, Vienna
(*) Corresponding Author


Loloh sembung (Blumea balsamifera) is a functional drink that is widely consumed by Balinesse people and exhibit health-promoting effect. The purpose of this study was to identify GABA of loloh sembung which has physiological effect as antihypertensive. Proximate analysis was performed to determine nutritional value content of loloh sembung. Atomic Absorption Spectrophotometric (AAS) method was employed to determine potassium and sodium content, and identificatio of ɤ-aminobutyric acid (GABA) using Thin Layer Chromatography (TLC) method. In this study, water content of loloh sembung was 99.2324%, ash 0.0117. Fresh sembung leaves have water content 83.8112%, ash content 2.0617%, protein 0.9659%, fat 2.850%, and carbohydrate 10.3109%. Loloh sembung contained sodium, 51.65 mg/L and potassium 1353.10 mg/L. Subsequently, fresh sembung leaves contained sodium 579.72 mg/L and pottasium 1353.19 mg/L respectively. In this study, GABA was detected in loloh sembung and has promising prospect as antihypertensive-functional drink.


Loloh sembung; nutritional value; GABA; AAS; TLC; functional drink; antihypertensive

Full Text:



Abe, Y., Umemura, S., Sugimoto, K.,Hirawa, N., Kato, Y., Yokoyama. (1995). Effect of Green Tea Rich in Gamma Aminobutyric Acid on Blood Pressure of Dahl salt-sesnsitive rats. American Journal of Hipertension, 8,74-79.

Akama, K., Kanetou,J., Shimosaki,S., Kawakami,K., Tsuchikura,S., & Takaiwa, F. (2009). Seed spesific expression of truncate OsGAD2 produces GABA-enriched rice grains that influence a decrease in blood pressure in spontaneously hypertensive rats. Transgenic Research, 18, 865-876.

Akihiro,T., Koike,S., Tani, R., Tominaga,T., Watanabe,S., Iijima,Y., & Ezura,H. (2008). Biochemical mechanism on GABA accumulation during fruit development in tomato. Plant and cell Physiology, 49 (9), 1378-1389.

Algridas, J., Januskevicience,G., Andruleviciute,V. (2012). Chemical Compostion and Energetic Values of Selected Vegetables Spicies in Lithuanian Supermarkets. Veterinarija Ir Zootechnika (Vet Met Zoot) 58 (80).

Amin, Md Zainul, Shariar,K., Sawraz,a.M., Satter,A. (2015) A Comparative Nutritional Analysis of Red Amarant (Amaranthus Tricolor L) on Refrigerating and nNn-Refrigerating Condition. International Journal of Green and Herbal Chemistry. Vol 4, No 3 243-255.

Aoki, H., Furuya, Y., Endo, Y., & Fujimoto, K. (2003). Effect of gamma aminobutyric acid-enriched tempeh-like fermented soybean (GABA-Tempeh) on the blood pressure of spontaneously hypertensive rats. Bioscience Biotechnology and Biochemisty, 67, 1806-1808.

Ayu Kusuma Wardani, I Gusti Agung., Ketut Agus Arianta. (2015). Efektivitas Ekstrak Etanol Daun Bayam Merah (Amaranthus tricolor) sebagai Deuretik pada Tikus Putih Jantan Galur Wistar (Rattus Hovergicus).

Duyff, RL. (2012). American Dietetic Association. Complete food and nutrition guld. Houghton miffle harcourt

Endrizzi, I., Pirretti, G., Calo,DG., Gaspen, F. (2009). A consumer study of fresh juice containing berry fruits. J.Sci Food Agric. 89 (7):1227-1235.

Hamauzu, Y. and Zhang, D. (2004). Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking. Food Chemistry, 88: 503-509.

Hayakawa, K, Kimura M, Kamata K. (2002). Mechanism Underlying gamma aminno butyric-acid –induced antihypertensive effect in spontaneously hypertensive rats. Eur.J.Pharmac.438,107-113.

Hayakawa,K., Kimura, M., & Yamori, Y. (2005). Role of the renal nerves in gamma aminobutyric acid- induced antihypertensive effect in spontaneously hypertensive rats. European Journal of Pharmacology. 524.120-125

Hayakawa,K., Kimura, M., Kasaha, K., Matsumoto, K., Sansawa, H., & Yamori, Y. (2004). Effect of gamma aminobutyric acid-enriched dairy product on the blood pressure of spontaneously hipertensive and normotensive wistar-Kyoto rats. British Journal of Nutrition,92, 411-417.

Indrowati,M., Pudji,A., Rarastoeti,P., Rumiyati. (2015). Deteksi Gamma-Aminobutyric Acid (GABA) Pada Daun Artocarpus Altilis. Seminar Nasional Pendidikan Sains FIKP UNS.

Joint National Commitee (JNC). (2003). Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. US Departemnet of Health dan Human Service.

Kimura, M., Hayakawa, K., &Sansawa, H. (2002). Involvement of gamma aminobutyric acid (GABA) B receptors in the hypotensive effect of systemically administered GABA in spontaneously hypertensive rats. The Japanese Journal of Pharmacology, 89,388-394.

Musa, A dan Ogbadoyi, E.O. (2012). Effect of Processing Methods on some Micronutrients, Antinutrients and Toxic Substance in Hibicus sabdariffa. Asian Journal of Biochemistry. 7 (2) : 63-79.

Nathalie, Lisbeth Leurs. 2009. Medical, Aromatic, and Cosmetic (MAC) Plants for Community Health and Bio-Cultural Diversity Conservation in Bali, Indonesia. L.N. Leurs page 124:127

Nielsen, S.S. (2010). Food analysis laboratoty manual. Department of Food Science. Purdue University West Lafayette. IN USA

Norikura, T., Kojima, Y.A., Shamizu,M., Huang. X.Xu, S., Kametami, S., Rho, SN., Kennedy, D.O., and Matsui, Y.I. (2008). Anticancer activities and mechanism of Blumea balsamifera exctract in hepatocellular carsinoma cells. Am J Chib Med. 36 (2):411-24

Qiu, T., Li,H., & Cao, Y. (2009). Pre-staining thin layer chromatography method for amino acid detection Afr J Biotechnol, 9, 8679-8681

Riset Kesehatan Dasar. (2013) Penyakit tidak menular. Halaman : 121-136

Sacks,F.M., Laura,F.S., Willia,M.V., lawrence,S.R., George,A.B., David,H., Eva,O., Paul,R.C., Edgar,R.M., Denise,G.S.M., Njeri,K., Pao-Hwa,L., Mikel,A., Marlene,M.M.W., Thomas,J.M., Michael,A.P., Jettrey,A.C. (2001). Effect of Blood Pressure of Reduce Dietary Sodium and The Dietary Approach to Stop Hypertension (DASH) Diet. The New England Journal of Medicine. 344 : 3-10.

Santos, P.C.J.L., Krieger, J.E., and Pireira, A.C. (2012). Renin agiotensin system hypertension and chronic disease. Pharmacogenetic implication Journal of Pharmacological science, 120:77-78.

sembung (Blumea balsamifera).

Winarno, F.G. (2008). Kimia Pangan dan Gizi. Bogor : Mrio Press.

Wita, I.G.A., Darmawijaya, I Putu., Yogeswara, I.B.A., Santoso, U., Marsono, Y. (2016). Formulasi dan kemampuan menangkal radikal bebas dari loloh

Wita, I.G.A., I.M Wisnu A.P., Yogeswara, I.B.A., Santoso, U., Marsono, Y. (2016). Antioxidannt and antihypertensive activity of loloh sembung (Blumea balsamifera). Traditional Medecine Journal. Vol 2. No 3.

Wita, I.G.A., Yogeswara, I.B. (2016). Antioxidant and Antibacterial Capacity of Loloh Sembung (Blumea balsamifera) based on Extraction Method. Trad.Med.J. Vol. 21 (3). Page 143-148.

Wita, I.G.A.,Darmawijaya, I Putu ., & Yogeswara, I.B.. 2014. Potensi aktioksidan loloh tempuyung (Sonchus arvensis L.) sebagai minuman fungsional. Prosiding Seminar Nasional Biologi FMIPA UNHI

Yamakoshi,J., Fukuda,S., Satoh, T., Tsuji R., Saito M. (2007). Antihypertensive and natriuretic effect of less sodium soy sauce containing gamma aminobutyric acid in spontaneously hipetensive rats. Bioscience Biotechnology and Biochemisty, 71, 165-173

Yang,N.C., Kun,Y.J., Chin,Y.T. (2012). Antihypertensive Effect of Mulberry Leaf Aqueous Extract Containing ɤ-Aminobutyric Acid in Spontaneously Hypertensive Rats. Food Chemistry 132.1796-1801

Yogeswara, I.B.A., Sumadewi, U., Rahayu, E., Indrati, R. 2017. Isolasi dan Skrining Bakteri Asam ` Laktat Isolat Asam ɤ-Aminobutirat. Abstrak.

Yoshimura,M., Toyoshi,T., Sano,A., Izumi,T., Fujii,T., Konishi,C. (2010). Antihypertensive effect of gamma amiobutyric acid rich tomato cultiver ‘DG03-9’ in


Article Metrics

Abstract views : 3138 | views : 6053


  • There are currently no refbacks.

Copyright (c) 2018 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada