The Effect of Pegagan (Centella Asiatica (L.) Urban) Ethanol Extracts on Hippocampal PSD-95 Protein Expression in Male Wistar Rats

Adibah Ferhad(1), Auliyani Andam Suri(2), Astri Handayani(3), Sri Redjeki(4*), Ria Kodariah(5)

(1) Biomedical Science Graduate Study Program, Faculty of Medicine, Universitas Indonesia
(2) Biomedical Science Graduate Study Program, Faculty of Medicine, Universitas Indonesia
(3) Biomedical Science Graduate Study Program, Faculty of Medicine, Universitas Indonesia
(4) Department of Physiology, Faculty of Medicine, Universitas Indonesia
(5) Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia
(*) Corresponding Author


One effort to overcome the decline in memory function is through herbal medicine. Pegagan (Centella asiatica (L.) Urban) contain the active components of triterpenoid and flavonoids, has been known to be able to improve memory function. Synaptic plasticity is the basis of memory formation which is strongly influenced by synapse proteins such as PSD-95. Loss of PSD-95 protein can cause memory function decline. This study aims to determine the effect of 70% ethanol extract of pegagan toward PSD-95 protein expression on hippocampus of male Wistar rat. Eighteen male Wistar rats were randomly divided into 3 groups, 6 rats/group: group (1) given ethanol extract of pegagan with dose 300 mg / kgBW / day (CA300), (2) given ethanol extract of pegagan with dose 600 mg / kgBW / day (CA600), and (3) control group (K), given daily aquadest. All three groups were treated for 28 consecutive days. At the end of the treatment period, rats were decapitated and the hippocampus was isolated from the brain. Analysis of protein was done by immunohistochemical method. Statistical analysis was performed by One Way ANOVA parametric test followed by Post-Hoc Bonferroni. The results showed that there was no significant difference between group K and group CA300 (P = 0.123), whereas there were significant differences between CA600 group and K group and CA300 group (P = 0.000). From this research, it can be concluded that 70% ethanol extract of pegagan with dose 600mg / kgBW / day can increase expression of PSD-95 protein on hippocampus of male Wistar rat.


Post Synaptic Density-95; memory function; flavonoids; Centella asiatica (L.) Urban

Full Text:



Anand, K. S. & Dhikav, V. 2012. Hippocampus in health and disease: An overview. Annals of Indian Academy of Neurology, 15, 239.

Andreollo, N. A., Santos, E. F. d., Araújo, M. R. & Lopes, L. R. 2012. Rat's age versus human's age: what is the relationship? ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo), 25, 49-51.

Bear, M. F., Connors, B. W. & Paradiso, M. A. 2016. Neuroscience: Exploring the Brain. , Philadelphia, Wolters Kluwer.

Bettio, L. E. B., Rajendran, L. & Gil-Mohapel, J. 2017. The effects of aging in the hippocampus and cognitive decline. Neurosci Biobehav Rev, 79, 66-86.

Chen, X., Levy, J. M., Hou, A., Winters, C., Azzam, R., Sousa, A. A., Leapman, R. D., Nicoll, R. A. & Reese, T. S. 2015. PSD-95 family MAGUKs are essential for anchoring AMPA and NMDA receptor complexes at the postsynaptic density. Proc Natl Acad Sci U S A, 112, E6983-92.

Ehrlich, I. & Malinow, R. 2004. Postsynaptic density 95 controls AMPA receptor incorporation during long-term potentiation and experience-driven synaptic plasticity. J Neurosci, 24, 916-27.

Gray, N. E., Harris, C. J., Quinn, J. F. & Soumyanath, A. 2016. Centella asiatica modulates antioxidant and mitochondrial pathways and improves cognitive function in mice. J Ethnopharmacol, 180, 78-86.

Han, K. & Kim, E. 2008. Synaptic adhesion molecules and PSD-95. Prog Neurobiol, 84, 263-83.

Jared, S. R. 2010. Enhancement of memory in rats with Centella asiatica. Biomedical Research, 21.

Leal, G., Afonso, P. M., Salazar, I. L. & Duarte, C. B. 2015. Regulation of hippocampal synaptic plasticity by BDNF. Brain Res, 1621, 82-101.

Lokanathan, Y., Omar, N., Puzi, N. N. A., Saim, A. & Idrus, R. H. 2016. Recent updates in neuroprotective and neuroregenerative potential of Centella asiatica. The Malaysian journal of medical sciences: MJMS, 23, 4.

Lu, Y., Christian, K. & Lu, B. 2008. BDNF: a key regulator for protein synthesis-dependent LTP and long-term memory? Neurobiol Learn Mem, 89, 312-23.

Luo, L., Liu, X. L., Mu, R. H., Wu, Y. J., Liu, B. B., Geng, D., Liu, Q. & Yi, L. T. 2015. Hippocampal BDNF signaling restored with chronic asiaticoside treatment in depression-like mice. Brain Res Bull, 114, 62-9.

Marlatt, M. W., Potter, M. C., Lucassen, P. J. & van Praag, H. 2012. Running throughout middle-age improves memory function, hippocampal neurogenesis, and BDNF levels in female C57BL/6J mice. Dev Neurobiol, 72, 943-52.

Mirza I, R. H., Khomsan A, Marliyati SA, Damayanthi E, Winarto A. 2013. Pengaruh ekstrak etanol daun pegagan (centella asiatica (l.)

urban) terhadap gambaran darah, aktivitas,

dan fungsi kognitif tikus. Jurnal Kedokteran Hewan, 7.

Nelson, T. J. & Alkon, D. L. 2015. Molecular regulation of synaptogenesis during associative learning and memory. Brain Res, 1621, 239-51.

Ojo, B., Rezaie, P., Gabbott, P. L., Davies, H., Colyer, F., Cowley, T. R., Lynch, M. & Stewart, M. G. 2012. Age-related changes in the hippocampus (loss of synaptophysin and glial-synaptic interaction) are modified by systemic treatment with an NCAM-derived peptide, FGL. Brain Behav Immun, 26, 778-88.

Orhan, I. E. 2012. Centella asiatica (L.) Urban: From Traditional Medicine to Modern Medicine with Neuroprotective Potential. Evid Based Complement Alternat Med, 2012, 946259.

Ramadhan, N. S., Rasyid, R. & Syamsir, E. 2015. Daya Hambat Ekstrak Daun Pegagan (Centella asiatica) yang Diambil di Batusangkar terhadap Pertumbuhan Kuman Vibrio cholerae secara In Vitro. Jurnal Kesehatan Andalas, 4.

Salamah, N. & Farahana, L. 2014. Uji Aktivitas Antioksidan Ekstrak Etanol Herba Pegagan (Centella asiatica (L.) Urb) dengan Metode Fosfomolibdat. Pharmaciana, 4.

Sari, D. C. R. & Rochmah, M. A. 2015. The effects of ethanol extracts of centella asiatica leaf on serial serum brain derived neurotrophin factor (bdnf) concentration of rats (sprague dawley) following chronic stress. KnE Life Sciences, 2, 159.

Shivarama Shetty, M. & Sajikumar, S. 2017. 'Tagging' along memories in aging: Synaptic tagging and capture mechanisms in the aged hippocampus. Ageing Res Rev, 35, 22-35.

Spencer, L. T. & Bancroft, J. D. 2008. Microtomy: paraffin and frozen. Dalam: Brancroft JD, Gamble M. Theory and practice of histological techniques. 6th ed. Philadelphia: Elsevier.

Tau, G. Z. & Peterson, B. S. 2010. Normal development of brain circuits. Neuropsychopharmacology, 35, 147-68.

Vauzour, D., Vafeiadou, K., Rodriguez-Mateos, A., Rendeiro, C. & Spencer, J. P. 2008. The neuroprotective potential of flavonoids: a multiplicity of effects. Genes Nutr, 3, 115-26.

Zhang, J., Lewis, S. M., Kuhlman, B. & Lee, A. L. 2013. Supertertiary structure of the MAGUK core from PSD-95. Structure, 21, 402-13.


Article Metrics

Abstract views : 3826 | views : 2700


  • There are currently no refbacks.

Copyright (c) 2018 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada