.png)
.png)
* Corresponding Author
Acute Tocixity of Sterculia quadrifida R.Br Bark Ethanol Extract on Sprague-Dawley Rats
https://doi.org/10.22146/mot.34871 Siswadi Siswadi(1*), Grace S. Saragih(2)
(1) Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Kupang
(2) Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Kupang
(*) Corresponding Author
Abstract
Faloak (Sterculia quadrifida R.Br) is a medicinal plant used by people in the province of East Nusa Tenggara to treat hepatitis. This study aims to determine the acute toxicity of faloak (Sterculia quadrifida R. Br) bark ethanol extract in Sprague-Dawley (SD) male white rats. The method used is experimental using post-test only control group design. In this study the parameters used are body weight, clinical symptoms, clinical biochemistry (ALT & AST), histopathology of liver organ and mortality in rats. Twenty male Sprague-Dawley rats were randomly assigned to 5 groups, group 1 as control, group 2 was administered orally faloak bark ethanol extract at dose of 40 mg / kg body weight, group 3 at dose of 200 mg / kg body weight, group 4 at dose of 1000 mg / kg body weight and group 5 at dose of 5000 mg / kg body weight. Results of observation for 24 hours there are no dead rats, so the value of LD50 extract ethanol faloak is > 5,000 mg / kg body weight. Observations were conducted for 14 days to observed delayed occurrence of toxic effects. Oral administration of the faloak bark ethanol extract reduced the level of AST and ALT. The histopathology observation of rats liver cells showed liver cell necrosis at treatment doses of 200 - 5,000 mg / kg body weight.
Keywords
Full Text:
PDFReferences
Adikara, I.P.A., Winaya, I.B.O. & Sudira, I.W. 2013, 'Studi histopatologi hati tikus putih (Rattus norvegicus) yang diberi ekstrak etanol daun kedondong (Spondias Dulcis G. Forst) secara oral', Buletin Veteriner Udayana.
Amalina, N. 2009, 'Uji toksisitas akut ekstrak valerian (Valeriana officinalis) terhadap hepar mencit BALB/C', Universitas Dipenogor.
Amin, A., Wunas, J. & Anin, Y.M. 2016, 'Uji Aktivitas Ekstrak Etanol Kulit Batang Faloak (Sterculia quadrifida R. Br) Dengan Metode DPPH (2, 2-diphenyl-1-picrylhydrazyl)', Jurnal Fitofarmaka Indonesia, vol. 2, no. 2, pp. 111–4.
Birt, D.F., Hendrich, S. & Wang, W. 2001, 'Dietary agents in cancer prevention: flavonoids and isoflavonoids', Pharmacology & therapeutics, vol. 90, no. 2–3, pp. 157–77.
BPOM 2014, Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 2 Tahun 2014 Tentang Organisasi dan Tata Kerja Unit Pelaksana Teknis di Lingkungan BPOM.
Castilhos, R. V, Grützmacher, A.D. & Coats, J.R. 2017, 'Acute Toxicity and Sublethal Effects of Terpenoids and Essential Oils on the Predator Chrysoperla externa (Neuroptera: Chrysopidae)', Neotropical entomology, pp. 1–7.
Deshpande, H.A. & Bhalsing, S.R. 2015, 'Sterculiaceae: A critical appraisal on plant tissue culture studies in medicinally important plants', Research in Biotechnology, vol. 6, no. 2.
Elisma, E., Handalia, E. & Arifin, H. 2011, 'Pengaruh Ekstrak Etanol Herba Ginseng Sumatera (Talinum triangulare (Jacq.) Willd) Terhadap Aktivitas SGOT Dan SGPT Pada Serum Darah Mencit Putih Jantan Yang Terinduksi Karbontetraklorida', Jurnal farmasi Higea, vol. 3, no. 1.
Eriadi, A., Arifin, H. & Nirwanto, N. 2017, Uji Toksisitas Akut Ekstrak Etanol Daun Kirinyuh (Chromolaenodorata (L) RM King & H. Rob) Pada Mencit Putih Jantan.
Esther, E., Smit, S., Beukes, M., Apostolides, Z., Pirk, C.W.W. & Nicolson, S.W. 2015, 'Detoxification mechanisms of honey bees (Apis mellifera) resulting in tolerance of dietary nicotine', Scientific reports, vol. 5, p. 11779.
Hafizah, I., Sudayasa, I.P., Uddu, W.S.A., Imran, M. & Yakin, A. 2017, 'Pengaruh Minuman Tradisional Kameko Terhadap kadar SGOT, SGPT, dan Jaringan Hati Mencit (Mus musculus)', Pharmauho, vol. 3, no. 1.
Ismiyah, F., Fauziyah, B., Fasya, A.G. & Muti’ah, R. 2014, 'Identifikasi Golongan Senyawa Dan Uji Toksisitas Akut Ekstrak Etanol 95% Daun, Kulit Batang Dan Akar Pulai (Alstonia scholaris (L.) R. Br.) Terhadap Mencit Balb/C', ALCHEMY: Journal of Chemistry, pp. 12–7.
Jayanegara, A. 2013, 'Potensi Tanin Sebagai Bahan Untuk Mitigasi Gas Metana Dari Ruminansia', Badan Litbang Pertanian, pp. 147–65.
Kementerian Kesehatan 2014, Situasi dan Analisis Hepatitis.
Longo, D. & Harrison, T.R. 2013, Harrison’s hematology and oncology, 2e, McGraw Hill Professional.
Mau, R. 2010, 'Ecosystem and community based model for Zonation in Nino Konis Santana National Park, Timor-Leste', Unpublished thesis, Institute Pertanian Bogor.
Siswadi 2017, Budidaya dan Pemanfaatan Faloak (Sterculia quadrifida R.Br.) Sebagai Tumbuhan Obat Potensial NTT, Kupang.
Siswadi, Raharjo, A.S., Pudjiono, E., Saragih, G.. & Rianawati, H. 2016, 'Pemanfaatan Kulit batang Faloak (Sterculia quadrifida R.Br.) sebagai bahan baku obat herbal di Pulau Timor', in G. Njurumana, S.A.. Rahardjo, M.. Riwu Kaho, H. Kurniawan & M. Hidayatullah (eds),Prosiding Seminar Nasional Biodiversitas Savana Nusa Tenggara, Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Kupang, Kupang.
Siswadi, Rianawati, H., Saragih, G.. & Sulistyo, D.H. 2014, 'The Potency of Faloak’s (Sterculia quadrifida, R.Br.) Active Compunds as Natural Remedy', in M. Rizal, N.M. Januawati, Y. Widyastuti, Brotokardono, R. Effendi, D. Rohadi & T. Herwati (eds),Proceeding International Seminar ‘Forests and Medicinal Plants for Better Human Welfare’., Bogor, pp. 73–9.
Soeksmanto, A., Simanjuntak, P. & Subroto, M.A. 2010, 'Uji toksisitas akut ekstrak air tanaman sarang semut (Myrmecodia pendans) terhadap histologi organ hati mencit', Jurnal Natur Indonesia, vol. 12, no. 2.
Sujono, T.A., Wahyuni, A.S., Da’i, M., Trisharyanti, I. & Kusumowati, D. 2015, Pengaruh Pemberian Ekstrak Etanol Meniran (Phyllanthus Niruri L) Selama 90 Hari terhadap Fungsi Hati Tikus, Universitas Muhammadiyah Surakarta.
Widowati, L., Winarno, M. & Intan, P.R. 2014, 'Toksisitas akut dan subkronis ramuan ekstrak kelor dan klabet sebagai pelancar ASI dan penambah gizi', Jurnal Kefarmasian Indonesia, vol. 4, no. 2, pp. 51–64.
DOI: https://doi.org/10.22146/mot.34871
Article Metrics
Abstract views : 3992
| 
views : 18326
Refbacks
- There are currently no refbacks.
Copyright (c) 2018 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada