The Effect of Dayak Onion Bulb Ethanol Extract (Sisyrinchium palmifolium L.) on Triglyceride Level and Aorta Histopathology in Diabetes Melitus White Rat Induced by Alloxan

https://doi.org/10.22146/mot.38051

I Gusti Agung Ayu Kusuma Wardani(1*), Fitria Megawati(2), Ni Made Dharma Shantini Suena(3)

(1) Diploma Degree of Pharmacy, Faculty of Pharmacy, Mahasaraswati Denpasar University, Denpasar, Bali, Indonesia
(2) Diploma Degree of Pharmacy, Faculty of Pharmacy, Mahasaraswati Denpasar University, Denpasar, Bali, Indonesia
(3) Diploma Degree of Pharmacy, Faculty of Pharmacy, Mahasaraswati Denpasar University, Denpasar, Bali, Indonesia
(*) Corresponding Author

Abstract


The high prevalence of diabetes mellitus in Indonesia has made antidiabetic treatment efforts increasingly popular. In addition, the many side effects caused by the use of chemical drugs, triggered the development of research on herbal therapy. The purpose of this study was to determine the effectiveness of Dayak onion bulb extract in reducing two-hour postprandial blood glucose, triglyceride levels, and the amount of lipid vacuoles in aorta blood vessels of diabetic rat. The long-term goal of this study is the creation of innovation products in the form of standardized herbs as complementary therapies that can be used daily by the community, so that the risk of morbidity can be reduced. The research design used was randomized control group pretest posttest design. The results of blood glucose analysis showed that there were significant differences between the positive control group with the 400 mg/KgBW and 800 mg/KgBW extract group, with a significance value of 0,000. In the extract group, 400 mg/KgBW with 800 mg/KgBW extract group showed no significant difference with a significance value of 0.390. The results of the analysis of triglyceride levels showed no significant difference between the positive control group with the 400 mg/KgBW extract group, with a significance value of 0.981. However, there were significant differences between the positive control group with the 800 mg/KgBW extract group with a significance value of 0.025. Between the 400 mg/KgBW and 800 mg/KgBW extract groups, there was a difference with a significance value of 0.024. Aorta histopathology results showed that there was a significant difference in the number of lipid vacuoles between the positive control group and the 400 mg/KgBW extract group and 800 mg/KgBW extract group. Based on the research, it can be concluded that the extract dose of 400 mg/KgBW is a dose that is able to influence the decrease in blood glucose levels, triglycerides and the amount of lipid vacuoles in the aorta blood vessels.

Keywords


glukosa darah; trigliserida; umbi bawang dayak; vakuola lemak; blood glucose; Dayak onion bulb; lipid vacuoles; triglycerides

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References

Arjadi, F., Susatyo, P., 2010, ‘Regenerasi Sel Pulau Langerhans Pada Tikus Putih (Rattus norvegicus) DiabetesYang Diberi Rebusan Daging Mahkota Dewa (Phaleriamacrocarp (scheff.) Boerl.)’, Medica Journal of Medicine and Health, Vol. 2 No. 2.

Cahyono, S.B., 2008, Gaya Hidup dan Penyakit Modern, Kanisius, Yokyakrta.

Ciulei, J., 1984, Metodology for Analisis of Vegetables and Drugs, Faculty of Pharmacy, Buchrest Rumania, 11-26.

Daliamartha, S., 2005, Ramuan Tradisional Untuk Pengobatan Diabetes Mellitus, Penebar Swadaya, Bogor.

Depkes RI., 1989, Materia Medika Indonesia V,

Departemen Kesehatan Republik Indonesia, Jakarta, Hal. 549-553.

Dhamuri, A.R., Fatimawali, Citranigtyas, G., 2014, ‘Uji Efektivitas Penurunan Kadar Gula Darah Ekstrak Etanol Daun Sendok (Plantago Major L.) Pada Tikus Putih Jantan Galur Wistar (Rattus Novergicus) Yang Diinduksi’, Jurnal Ilmiah Farmasi – UNSRAT, Vol. 3 No. 2.

Direktorat Jenderal POM RI., 2000, Parameter Standar Daun Ekstrak Tumbuhan Obat, Jilid 1. Departemen Kesehatan Republik Indonesia, Jakarta.

Fahri, C., Sutarno, Listyawati, S., 2005, ‘Kadar Glukosa dan Kolesterol Total Darah Tikus Putih (Rattus norvegicus L) Hiperglikemik setelah Pemberian Ekstrak Metanol Akar meniran (Phyllanthus niruri L)’, Biofarmasi, 3 (1).

Farnworth, N. R., 1966, ‘Biological and Phytochemical Screening of Plant’, Journal of Pharmaceutical Sciences, 55:59.

Fatmawati, E., 2008, ‘Pengaruh Lama Pemberian Ekstrak Daun Sambiloto (Andrographis paniculata Ness.) Terhadap Kadar Kolesterol LDL (Low Density Lipoprotein), HDL (Hight Density Lipoprotein) dan Trigliserida Darah Tikus (Rattus norvegicus) Diabetes’, Skripsi, Falkutas Sains dan Teknologi Universitas Islam Negeri, Malang.

Febrinda, A. E., 2013, ‘Kapasitas Antioksidan dan Inhibitor Alfa Glukosidase Ekstrak Umbi Bawang Dayak’, Jurnal Teknologi dan Industri Pangan, Vol. 24 No. 2.

Guyton, A.C. dan Hall, J.E., 2007, Buku Ajar Fisiologi Kedokteran, EGC, Jakarta.

Jones, W. P., Kinghorn, A. D., 2006, Extraction of Plant Secondary Metabolites. In : Sharker, S.D. Latif Z., Gray A.L, eds. Natural Product Isolation, 2nd Edition, Humana Press, New Jersey, Pp. 341342.

Jung, Chang, H., dkk., 2006, ‘Antihyperglycemic Activity of Herb Extract on Streptozotocin-Induced Diabetic Rats’, Biosci, Biotechnol, Biochem, (10) 70 : 2556-2559.

Karunia, P. B., Winarso, D., Trisunuwati, P., 2014, ‘Pengaruh Ekstrak Ethanol Curcuma Longa L. Sebagai Terapi Diabetes Mellitus 1 pada Tikus Model Induksi Streptozotocin Terhadap Kadar Trigliserida dan Gambaran Histopatologi aorta’, Student Journal, Vol 3. No. 4

Koeswono, Y.E, 2015, ‘Uji Aktivitas Antidiabetes Ekstrak Etanol Daun Kluwih (Artocarpus camansi) dan Gambaran Histologi Pankreas Mencit Jantan Yang Diinduksi Aloksan’, Skripsi, Fakultas Farmasi, Universitas Jember.

Kumalaningsih, 2007, Antioksidan dan Penangkal Radikal Bebas, Penerbit Trubus Agrisarana, Jakarta.

Latha, RCR., Daisy P., 2011, ‘Insulin secretagogue, antihyperlipidemic and other protective effects of gallic acid isolated from Terminalia bellerica Roxb. in streptozotocininduced diabetic rats’, Chem. Biol. Interact, 189: 112-118.

Mansjoer, A. dkk., 2008, Kapita Selekta Kedokteran, Media Aesculapius, Jakarta.

Nugroho, A. E., 2006, ‘Animals Models of Diabetes mellitus: Pathology and Mechanism of Some Diabetogenics’, Biodiversitas, Volume 7 Nomor 4.

Peng, I-Wen dan Kuo, S.M., 2003, ‘Flavonoid Structure Affects the Inhibition of Lipid Peroxidation in Caco-2 Intestinal Cells at Physiological Concentrations’, The American Society for Nutritional Sciences J. Nutr, 133:2184-7

Purwanto, H., 1994, Komunikasi Untuk Perawat, EGC, Jakarta.

Raissa, U. F., 2014, ‘Antidiabetic Effect of Morinda Citrifolia L. As A Treatment of Diabetes Mellitus’, Medical Journal of Lampung University, Volume 3 Nomor 7.

Ridwan, E., 2013, ‘Etika Pemanfaatan Hewan Percobaan dalam Penelitian Kesehatan’, J. Indon. Med. Assoc., 63 (3): 112-6

Robinson, T., 1991. Kandungan Organik Tumbuhan Tingkat Tinggi, Penerbit ITB. Bandung, P. 152-196.

Sulistyoningrum, E., Kautsari, S., Susatyo, P., 2010, ‘Tinjauan Histologis Pembuluh Darah Tikus Putih (Rattus norvegicus) Diabetes yang diberi rebusan Daging Buah Mahkota Dewa’, J. Mandala of Health. vol 4(2): 92-95.

Taylor, R.B., David, A.K., Philips, D.M., Fields, S.A., Scherger, J.E., 2005, Cardiovascular Disease, Springer Science and Business Media, New York.

Wibowo, T., 2009, ‘Pengaruh Pemberian Seduhan Kelopak Rosela (Hibiscus Sabdariffa) Terhadap Kadar Trigliserida Darah Tikus Putih (Rattus Norvegicus)’, Skripsi, Universitas Sebelas Maret, Surakarta.

Widyastuti, S.K., dkk., 2000, ’Monyet Ekor Panjang (Macaca fascicularis) sebagai Model Diabetes Mellitus: Pengaruh Hiperglikemia pada Lipid Darah, Serum Oksida Nitrit (NO), dan Tingkah Laku’, Tesis, Program Pascasarjana Institut Pertanian Bogor, Bogor.

Wiztum, J.L., Steinberg, D., 1991, ‘Role of Oxidized Low Density Lipoprotein in Atherogenesis’, J. Clin. Invest., 88: 1785-1792.



DOI: https://doi.org/10.22146/mot.38051

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