Immunomodulatory Activity of Combination of Phyllanthus niruri Linn, Typhonium flagelliforme (Lodd.) Blume, and Piper crocatum on Macrophage Phagocytosis In Vitro

https://doi.org/10.22146/mot.46705

Reynelda Juliani Sagala(1), Retno Murwanti(2*), Andayana Puspitasari Ghani(3), Agustinus Yuswanto(4)

(1) Department of Pharmacy, Faculty of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Research on the activity of Phyllanthus niruri Linn, Typhonium flagelliforme (Lodd.) Blume and Piper crocatum have been conducted and showed various immunomodulatory activity. This study aims to investigate the immunomodulatory activity of the combination of the ethanolic extracts of Phyllanthus niruri Linn, Typhonium flagelliforme (Lodd.) Blume, and Piper crocatum by determining its macrophages phagocytic index and macrophages phagocytic capacity. Therefore, such a combination could be an alternative drug to increase immune response. In this study, the extraction procedure was carried out through maceration by using an ethanolic solvent. Combinations of herbs ethanol extract were varied in four groups of combination, at three different concentrations of 1 μg/ml, 10 μg/ml, and 100 μg/ml for each group. Macrophages were isolated from the peritoneum cavity of male mice (Mus musculus), and its phagocytic activity was quantified through the Leijh method (1986). The phagocytic index and phagocytic capacity of macrophages were determined by using latex beads as a trigger of phagocytosis and compared with negative controls of media, DMSO, and four groups of ethanolic extract combinations in different concentrations. The results indicate that all of combination group ethanol extract with a concentration of 10 μg/ml was significantly (p<0.05) optimum activated phagocytic index. Therefore the combination of Phyllanthus niruri Linn, Piper crocatum, and Thyphonium flagelliforme (Lodd.) Blume ethanolic extract might be prospective to increase nonspecific immune response.


Keywords


Phyllanthus niruri Linn; Thyphonium flagelliforme (Lodd.) Blume and Piper crocatum; macrophages phagocytic index; macrophages phagocytic capacity; immunomodulator activity

Full Text:

PDF


References

Apriyanto,2011,Uji Aktivitas Immunomodulator Ekstrak etanolik Daun sirih Merah (Piper crocatum) Terhadap Proliferasi Sel Limfosit dan Fagositosis Makrofag Pada Tikus yang Diinduksi Vaksin Hepatitis B,Thesis,Gadjah Mada University, Yogyakarta, Indonesia.

Baratawidjaja, K. and Rengganis, I,2010,Imunologi Dasar, 9thed. Jakarta: Balai Penerbit Fakultas Kedokteran Universitas Indonesia.

Daulay,2012,Efek Imunomodulator Ekstrak Etanolik Umbi Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Terhadap Perubahan Kadar IL-10 dan TNF-α pada Tikus Jantan Wistar Terinduksi Cyclophosphamide,Thesis,Gadjah Mada University, Yogayakarta, Indonesia.

Departemen Kesehatan Republik Indonesia,1986,‘Sediaan Galenik’, Sediaan Galenik, pp. 7–10.

Departemen Kesehatan Republik Indonesia,2000,Parameter Standard Umum Ekstrak Tumbuhan Obat. Cetakan pe. Jakarta: Departemen Kesehatan Republik Indonesia.

Galuh, A,2008,‘Pengaruh Pemberian Phyllanthus niruri L Terhadap Fungsi Fagositosis Makrofag Pada Mencit BALB/C Yang Diinfeksi Salmonella Typhimurium’, pp. 3–13.

Ibnul,2012,Uji Komparasi Aktivitas Fagositosis Makrofag dan Produksi Nitrit Oksida pada Mencit Balb/c Akibat Perlakuan Ekstrak Meniran Hijau (Phyllanthus niruri) dan Meniran Merah (Phyllanthus urinaria) yang Diinfeksi Bakteri Salmonella thypi,Thesis,Universitas Sebelas Maret (UNS), Solo, Indonesia.

Jensch-Junior, B. . et al,2006,‘Characterization of Macrophage Phagocytosis of the Tropical Fish Prochilodus scrofa (Steindachner, 1881)’, Aquaculture, 251, pp. 509–515.

Leijh, P.C.., R.V Furth, dan Zwet, T.L.V, 1986. In vitro determination of phagocytosis and intracelullar killing by polymorphonuclear and mononuclear phagocytes, dalam: Cellular Immunology. hal. 46, Blackwell Scientific Publication, London.

Maat, S,1996,Phyllanthus niruri Linn sebagai imunostimulator pada mencit,Disertasi,Universitas Airlangga.

Middleton, E. J., Kandaswami, C. and Theoharides, T,2000,‘The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer’, Pharmacol Rev, 52(4), pp. 673–751.

Nobakht et al,2010,‘Analysis of preliminary phytochemical screening of Typhonium flagelliforme’, African Journal of Biotechnology, 9(11), pp. 1655–1657.

Nugrahani, S,2013,'Analisis Perbandingan Efektifitas Ekstrak Akar, Batang, dan Daun HErba Meniran dalam Menurunkan Kadar Glukosa Darah Mencit‘Unnes Journal of Public Health’, 2(1).

Optimization, T. et al,2018,‘Optimasi Ekstrak Etanol Daun Sirih Merah ( Piper Crocatum ) sebagai Antibakteri terhadap Bakteri Staphylococcus Aureus’, 18(1), pp. 13–19.

Shen, W.-C. and Louie S,2005, Inmunology for Pharmacy Students, 44-48 Harwood Academic Publishers, USA

Sherwood, L,2012,Introduction to Human Physiology, Cengage Learning/Brooks/Cole. Brooks/Cole

Sianipar, N. F., Ariandana and Maarisit, W,2015,‘Detection of Gamma-Irradiated Mutant of Rodent Tuber (Typhonium Flagelliforme Lodd.) in vitro Culture by RAPD Molecular Marker’, Procedia Chemistry. Elsevier Ltd., 14, pp. 285–294.

Ukhrowi, U,2011,Pengaruh Pemberian Ekstrak Etanol Umbi Bidara Upas (Merremia mammosa) terhadap Fagositosis Makrofag dan Produksi Nitrit Oksida (NO) Makrofag,Thesis, Magister Ilmu Biomedik, Universitas Diponegoro, Semarang

Wiweko,2010,Uji Aktivitas Imunomodulator Ekstrak Etanol Daun Sirih Merah (Piper crocatum Lmk.) Terhadap Peningkatan Titer Imunoglobulin G dan Histopatologi Tikus yang Diinduksi Vaksin Hepatitis B,Thesis,Gadjah Mada University, Yogyakarta, Indonesia.

Yuristiyani,2012,Potensi Fraksi Tak Larut n-Heksana Ekstrak Etanol Daun Sirih Merah (Piper crocatum Ruiz & Pav.) Secara In vitro Sebagai Imunomodulator Terhadap Fagositosis Makrofag dan Proliferasi Sel Limfosit Tikus yang Diinduksi Phytohemagglutinin,Thesis, Gadjah Mada University, Yogyakarta, Indonesia.





DOI: https://doi.org/10.22146/mot.46705

Article Metrics

Abstract views : 3094 | views : 3154

Refbacks





Copyright (c) 2020 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada