Optimization of Self-Micro Emulsifying Drug Delivery System) for Soursop Leaf (Annona muricata Linn.) Chloroform Extract

https://doi.org/10.22146/mot.51590

Anif Nur Artanti(1*), Fea Prihapsara(2), Dian Eka Ermawati(3), Aprilia Saefanan Shofa(4)

(1) Division of Pharmacy, Sekolah Vokasi, Sebelas Maret University
(2) Department of Pharmacy, Matematic and Sains Faculty of Sebelas Maret University
(3) Division of Pharmacy, Sekolah Vokasi, Sebelas Maret University
(4) Department of Pharmacy, Matematic and Sains Faculty of Sebelas Maret University
(*) Corresponding Author

Abstract


Soursop leaf chloroform extract has anticancer activity.  The active ingredient of soursop leaf was acetogenin polypoid derivatives that have a lipophilic characteristic, and less effective to achieve action targets of drugs in biological systems. The Self-Micro Emulsifying Drug Delivery System (SMEDDS) was an effective drug delivery technique that increases the solubility of lipophilic drugs. This study aims to determine the proportion of optimum SMEDDS formula using Simplex Lattice Design (SLD) method. The Formula of SMEDDS was prepared using a combination of Tween 80-Croduret, Propylene Glycol, and Candlenut oil. Optimization formula with SLD method using Design-Expert software based on physical stability parameters there are the percent of transmittance and emulsification time. The optimum formula of SMEDDS was compared with SLD prediction formula using a statistical analysis t-test, then test of loading dose extract, stability test accelerated by centrifugation, particle size, and zeta potential. The proportion of optimum composition of Tween 80-Croduret, Propylene Glycol, and Candlenut oil of SMEDDS was 60.87%; 24.13%; 15.00% respectively. Results of transmittance 41.14±3.78% and emulsification time 119.0±2.08 seconds. The predicted SLD value for the transmittance percent was 55.0% and the emulsification time was 119.59 seconds. The result of the statistical analysis of one sample t-test showed no significant difference between observation results and SLD prediction. The SMEDDS system has F value of 0.99 and capable to load 25.0 mg chloroform extract of soursop leaf each system with an average particle size of 440 nm and zeta potential of +21.5 mV.

 


Keywords


SMEDDS; SLD; soursop leaf; chloroform extract

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References

Ahmad, J., Mir, S.R., Kohli, K., dan Amin, S., 2014. Effect of oil and cosurfactant on the formation of Solutol HS 15 based colloidal drug carrier by Box–Behnken statistical design. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 453: 68–77.

Ako, H., Kong, N., dan Brown, A., 2005. Fatty acid profiles of kukui nut oils over time and from different sources. Industrial Crops and Products, 22: 169–174.

Akula, S., Aravind K.G., dan Srinivas R.D. 2014. Self-Microemulsifying Drug Delivery Systems: An Attractive Strategy for Enhanced Therapeutic Profile. Hindawi Publishing Corporation International Scholarly Research Notices, Vol. 2014.

Astirin, O.P., Anif N.A., Meutia S.F., Eva A.P., dan Adi P. 2013. Annona muricata Linn Leaf Induce Apoptosis in Cancer Cause Virus. Journal of Cancer Therapy, Vol. 4, 1244-1250.

Astirin, O.P., Adi P., Anif N.A., Meutia S.F., Witianingsih D.A., Pranatami D.A., dan Putra S.T. 2014. The Expression of P53 and hsp 70 proteins after treatment with Annona muricata Linn leaf for activating apoptotic and lead to homeostatis program of Raji cells. Int. J. Cancer Ther Oncol, Vol. 2(2), 02028.

Avachat, A.M. dan Patel, V.G., 2014. Self nanoemulsifying drug delivery system of stabilized ellagic acid–phospholipid complex with improved dissolution and permeability. Saudi Pharmaceutical Journal,

Balakumar, K., Raghavan, C.V., selvan, N.T., prasad, R.H., dan Abdu, S., 2013. Self nanoemulsifying drug delivery system (SNEDDS) of Rosuvastatin calcium: Design, formulation, bioavailability and pharmacokinetic evaluation. Colloids and Surfaces B: Biointerfaces, 112: 337–343.

Bandyopadhyay, S., Katare, O.P., dan Singh, B., 2012. Optimized self nanoemulsifying systems of ezetimibe with enhanced bioavailability potential using long chain and medium chain triglycerides. Colloids and Surfaces B: Biointerfaces, 100: 50–61.

Bolton, S. dan Bon, C., 1997. Pharmaceutical Statistics: Practical and Clinical Applications. Dekker Media.

Dash, R.N., Mohammed, H., Humaira, T., dan Ramesh, D., 2015. Design, optimization and evaluation of glipizide solid self-nanoemulsifying drug delivery for enhanced solubility and dissolution. Saudi Pharmaceutical Journal, 23: 528 540.

De Melo, J.G., Thiago Antônio de S.A., Valérium T.N. de AeC, Daniela L. de V.C., Maria do D.R., Silene C. do N., Elba L.C. de A. dan Ulysses P. de A.. 2010. Antiproliferative activity, antioxidant capacity and tannin content in plants of semi-arid Northeastern Brazil. Molecules (15), 8534-8542.

Departemen Kesehatan, R., 2009. Materia Medika Indonesia, I. Departemen Kesehatan, Republik Indonesia : Jakarta.

Dewangga, VS. 2015. Karakterisasi Isolat Aktif Daun Sirsak (Annona muricata L.) dan Uji Sitotoksisitas Isolat Aktifnya terhadap Sel Hela. Universitas Sebelas Maret, Tesis.

Kassem, A.A., Mohsen, A.M., Ahmed, R.S., dan Essam, T.M., 2016. Selfnanoemulsifying drug delivery system (SNEDDS) with enhanced solubilization of nystatin for treatment of oral candidiasis: Design, optimization, in vitro and in vivo evaluation. Journal of Molecular Liquids, 218: 219–232.

Khan, B.A., Akhtar, N., Khan, H.M.S., Waseem, K., Mahmood, T., Rasul, A., dkk., 2011. Basics of pharmaceutical emulsions: A review. African Journal of Pharmacy and Pharmacology, 5: 2715–2725

Kuentz, M., 2011. Oral self-emulsifying drug delivery systems, from biopharmaceutical to technical formulation aspects. Journal of Drug Delivery Science and Technology, 21: 17–26.

Liaw, C.C., Chang F.R., Lin C.Y., Chou C.J., Chiu H.F., Wu M.J., dan Wu Y.C. 2002. New cytotoxic monotetrahydrofuran Annonaceous acetogenins from Annona muricata, J. Nat. Prod. Vol. 65 (4), 470–475.

Lynch, N. dan Berry, D., 2007. Differences in perceived risks and benefits of herbal, over-the-counter conventional, and prescribed conventional, medicines, and the implications of this for the safe and effective use of herbal products. Complementary Therapies in Medicine, 15: 84–91.

McClements, David Julian. 2012. Nanoemulsions versus microemulsions: terminology, differences, and similarities. The Royal Society of Chemistry, Vol. 8, 1719–1729.

Mc Laughlin. 2008. Paw-paw and Cancer Annonaceous Acetogenin from Discovery to Comercial Products. Department of Medicinal Chemistry and Molecular Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Purdue University, Vol. 71 (7),1311–1321.

Patel, D.M., Patel, N.M., Pandya, N.N., dan Jogani, P.D., 2007. Gastroretentive drug delivery system of carbamazepine: Formulation optimization using simplex lattice design: A technical note. AAPS PharmSciTech, 8: E82–E86.

Patel, J., Kevin, G., Patel, A., Raval, M., dan Sheth, N., 2011. Design and development of a self-nanoemulsifying drug delivery system for telmisartan for oral drug delivery. International journal of pharmaceutical investigation, 1: 112.

Paulinus, O.N., Agu K., dan Eze G.I. 2013. Protective Effect of Ethanolic Leaf Extract of Annona muricata Linn on some Early Events in Cycas-Induced Colorectal Carcinogenesis in Rats. Journal of Pharmaceutical and Scientific Innovation.`

Rao, S.V.R., Agarwal, P., dan Shao, J., 2008a. Self-nanoemulsifying drug delivery systems (SNEDDS) for oral delivery of protein drugs. International Journal of Pharmaceutics, 362: 10–15.

Rao, S.V.R., Yajurvedi, K., dan Shao, J., 2008b. Self-nanoemulsifying drug delivery system (SNEDDS) for oral delivery of protein drugs: III. In vivo oral absorption study. International Journal of Pharmaceutics, 362: 16–19.

Saryanti, D., Martodihardjo, S., Martien, R., 2016.

Optimasi SNEDDS Ekstrak Purwoceng Gunung (Artemisia lactiflora wall ex. DC) dan Uji Absorbi secara In Vitro. Tesis. Fakultas Farmasi : Universitas Gadjah Mada. Yogyakarta.

Senapati, P.C., Sahoo, S.K., dan Sahu, A.N., 2016. Mixed surfactant based (SNEDDS) self-nanoemulsifying drug delivery system presenting efavirenz for enhancement of oral bioavailability. Biomedicine & Pharmacotherapy, 80: 42–51.

Wang, L., Dong, J., Chen, J., Eastoe, J., dan Li, X., 2009. Design and optimization of a new self-nanoemulsifying drug delivery system. Journal of Colloid and Interface Science, 330: 443–448.

Wang, S., Su, R., Nie, S., Sun, M., Zhang, J., Wu, D., dkk., 2014. Application of nanotechnology in improving bioavailability and bioactivity of dietderived phytochemicals. The Journal of Nutritional Biochemistry, 25:363–376.

Zuhud, E.A. 2011. Bukti Kedahsyatan Sirsak Menumpas Kanker. Agromedia Pustaka, Jakarta.




DOI: https://doi.org/10.22146/mot.51590

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