Potential of Miana Leaf Extract as Expectorant (Profile Place of Growing, Antioxidant, Sputum Contaminants, Antibacterial, MIC, MKC Expectorant)

https://doi.org/10.22146/mot.52500

Sesilia Rante Pakadang(1), Santi Sinala(2*), Alfrida Monica Salasa(3), St Ratnah(4), Sisilia Teresia Rosmala Dewi(5), Maria Hilaria(6)

(1) Poltekkes Kemenkes Makassar
(2) Poltekkes Kemenkes Makassar
(3) Poltekkes Kemenkes Makassar
(4) Poltekkes Kemenkes Makassar
(5) Poltekkes Kemenkes Makassar
(6) Poltekkes Kemenkes Kupang
(*) Corresponding Author

Abstract


Research has been conducted on the treatment of phlegm cough with miana leaf extract in vitro (effective dose of miana leaf extract as an expectorant and antibacterial agent causing cough with phlegm). The study aims to compare the antioxidant activity of miana leaves from 3 locations where it grows, determine the types of contaminant bacteria in the sputum of cough sufferers, determine the minimum value of inhibitor concentration (MIC) and MKC of miana leaves against the test bacteria causing cough with phlegm, determine the effective dose of miana leaves that can used as a reference for coughing up phlegm and proving the potential of miana leaves as a sputum thinner. Miana leaf extraction is done by the juicer method. Antioxidant activity testing uses the DPPH method. Determination of test bacteria is done by isolating and identifying contaminant bacteria in the sputum sample of cough with phlegm. Testing the effectiveness of miana leaves against test bacteria is determined by the liquid dilution method. Expectorant activity testing was determined by measuring the viscosity of mucus viscosity of cow intestine treated with miana leaf extract. The results found that antioxidant activity was influenced by the location where miana leaves grew with an antioxidant potential of IC72 0.072 mg/ml - 0.76 mg/ml. Contaminant bacteria from sputum samples of cough patients are Streptococcus pneumonia, Klebsiella pneumonia, Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter agglomerans. MIC values for contaminant bacteria are 0.1% - 0.75% and MKC values are found between 0.25% - 1.75%. miana leaf extract has the potential as a sputum thinner at a concentration of 0.01% - 0.1%. The recommended dose of miana leaf extract as a cough with phlegm is 1.75% w/v.


Keywords


miana leaves; sputum contaminant bacteria; antioxidants; expectorants; MIC; MKC

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References

Abdollahzadeh SH, Masouf RY, Mortazavi H, Moghaddam MH, Roozbahani N, Vahedi M. Antibacterial and Antifungal Activities of Punica Granatum Peel Extracts Againts Oral Pathogens. Tehran University of Med Sci J Dentistry. 2011; 8(1): 1-6.

Altiner A, Wilm S, Daubener W, Bormann C, Pentzek M, Abholz H, et al. Sputum colour for diagnosis of a bacterial infection in patient with acute cough. Scandinavian journal of primary health care 2009;1-4

Alshawsh, M., Abdulla, M., Ismail, S., Amin, Z. (2012) Free Radical Scavenging, Antimicrobial and Immunomodulatory Activities of Orthosiphon stamineus. Journal of Molecules ISSN 1420-3049, 5385-5395.

Bannister, B., Gillespie, S., Jones, J. (2006) Infection Microbiology and Management. Third edition, Published by Blackwell Publishing Asia Pty Ltd, Victoria Australia.

Baratawidjaja, K.G., Rengganis, I. (2010) Imunologi Dasar. edisi ke-10. Jakarta: Badan Penerbit Fakultas Kedokteran Universitas Indonesia, hal 21,32-43,62-63,74,219,401-402,518-519,524,546-547,651,649,681.

Cushnie TPT, Lamb AJ. Antimicrobial Activity of Flavonoids. Int J Antimicrob Agents. 2005; 26: 343-356

Danusantoso, H., 2000, Anamnesis Penyakit Paru, In: Rachmah L, editor, Buku saku Ilmu Penyakit Paru, Hipokrates, Jakarta, p.7-12

Farming.id, 2017, Green Indonesian Project, https://farming.id/faktor-faktor-yang-mempengaruhi-pertumbuhan-dan-perkembangan-tanaman/

Hanani, E, Mun’im A, Sekarini, R, dan Wiryowidagdo, S. (2006) Uji aktivitas antioksidant beberapa spons laut dari kepulauan Seribu, Jurnal Bahan Alam Indonesia, vol 5, no.1 Jan 2006 (in Press).

Hanani,E., 2016, “Analisis Fitokimia” Penerbit : Buku Kedokteran EGC; Jakarta.

Ibrahim M. Sputum culture [homepage on the Internet]. 2012 [updated 2012 Aug 13]. Available from: http://emedicine.medscape.com/article/2119232-overview.

Islam B, Khan AN, Khan AU. Dental Caries: From Infection to Prevention. Med Sci Monit. 2007; 13 (11):196-203.

Karlina CY, Ibrahim M, Trimulyono G. Aktivitas Antibakteri Ekstrak Herba Krokot (Portulaca oleracea L.) terhadap Staphylococcus aureus dan Escherichia coli. Lentera Bio. 2013; 2 (1):87-93.

Kemenkes RI (2013) Pedoman Teknologi Formulasi Sediaan Berbasis Ekstrak vol 2, Badan Pengawasan Obat dan Makanan, Jakarta. Hal 5-12.

Mutiatikum, D., Alegantina, S. dan Astuti, Y. (2012) Standardisasi Simplisia Dari Buah Miana (Plectranthus Seutellaroides (L) R.Bth ) Yang Berasal Dari 3 Tempat Tumbuh Menado, Kupang Dan Papua, Buletin Penelitian dan Pengembangan Kesehatan. Vol 38. No.1 hal 1-16

Pakadang, SR., 2015, Potensi Ekstrak Daun Miana (Coleus scutellarioides (L.) Benth.) sebagai Imunomodulator terhadap Tikus Model yang Terinfeksi Mycobacterium tuberculosis, Disertasi, Universitas Airlangga, Surabaya.

Pakadang, SR., 2014, Sensitivitas M. Tb terhadap Ekstrak Etanol Daun Miana (C. Scutellarioides (L.) Benth), Jurnal Farmasi dan Bahan Alam ISSN 2338-0616 vol 2, no. 2, Sept 2014

Pakadang, SR., Dewi, STR., Lopak, Y., 2015 Etnofarmakologi Tumbuhan Obat untuk Tuberkulosis Pada Suku Toraja di Sulawesi Selatan, oral presentasi pada simposium nasional kesehatan masyarakat ke-1, FKM, Universitas Airlangga, Surabaya.

Pakadang, SR. dan Karim, D., 2016, Inventorization and ethnopharmacology plant for treatment of infectious diseases in Gowa and Maros district of South Sulawesi Province, International Conference and Workshop on Pharmacy and Statistics; Current Development of Medicinal Plants and Biostatistics by Tadulako University, Palu, Indonesia

Panggalo, JT., Porotuo, J, Buntuan, V., 2013,

Identifikasi Bakteri Aerob pada Penderita Batuk Berdahak di Poliklinik Interna BLU RSUP Prof. dr. RD Kandou, Manado, Jurnal e-Biomedik (eBM), Volume 1, Nomor 1, Maret 2013, hlm. 408-413

Ringel, E., 2009, Pendekatan terhadap pasien dengan penyakit paru. In: Onion DK, editor, Buku Saku Hitam Kedokteran Paru, alih bahasa, Melfiawati, PT Indeks, Jakarta, p.10

Richardson MD., Warnock, DW., 2003, Fungal Infection Diagnosis and Management, third edition, Blackwell Publishing Massachusetts, USA.p 19, 29-30

Ryan, KJ., Ray, CG., 2004, Sherris Medical Microbiology. An introduction to infectios disease, fourth edition, Mc Graw Hill, Medical Publishing Division, New York, USA. p.661

Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of Medicinal Plants. J Pharmacog Phytochem. 2013; 1 (6): 168-182.

Scott JR, Barnett TC. Surface Proteins of GramPositive Bacteria and How They Get There. Annu Rev Microbiol. 2006; 60: 397-423.

Tan, H.T., Rahardja, K. (2010). Obat-Obat Penting: Khasiat, Penggunaan dan Efek-Efek Sampingnya, Edisi Kelima, PT. Elex Media Komputindo. Jakarta. Hal 5.

Vasconcelos LCS, Sampaio FC, Sampaio MCC, Pereira MSV, Higino JS, Peixoto MHP. Minimum Inhibitory Concentration of Adherence of Punica granatum Linn (Pomegranate) Gel Againts S. mutans, S. mitis, and C albicans. Braz Dent J. 2006;17(13):223-227.

Venkatesha, S., Rajaiah, R., & Berman, B. (2011) Immunomodulation of Autoimmune Arthritis by Herbal C AM. Evidence Based Complementary and Alternative Medicine, 1-13.

Ziyade N, Yagci A. Improving sputum culture result for diagnosis of lower respiratory tract by saline washing. Marmara medical journal. 2010;23:30-6.



DOI: https://doi.org/10.22146/mot.52500

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