Cytotoxicity and Antiobesity Activity of Freeze-Dried Malus domestica, Canarium sp. and Averrhoa bilimbi Fruit

https://doi.org/10.22146/mot.52902

Sri Utami(1*), Susi Endrini(2), Said Nafik(3), Seila Arumwardana(4), Rizal Rizal(5), Dwi Surya Artie(6), Dewani Tediana Yusepany(7), Hanna Sari Widya Kusuma(8), Wahyu Widowati(9)

(1) Faculty of Medicine, YARSI University, Jakarta
(2) Faculty of Medicine, YARSI University, Jakarta, Indonesia
(3) Directorate General of Intellectual Property, Ministry of Law and Human Rights, Republic of Indonesia, South Jakarta, Indonesia
(4) Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
(5) Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Kampus UI, Kukusan, Beji, Depok 16424, West Java, Indonesia Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
(6) Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
(7) Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
(8) Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia
(9) Medical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia.
(*) Corresponding Author

Abstract


Obesity has a role in the development of diseases such as diabetes, cardiovascular disease and hyperlipidemia which is characterized by the increase of adipose tissue mass due to an imbalance of energy intake and expenditure. Freeze-dried fruits are well known to possess antiobesity activity. In this study, we have evaluated the antiobesity activity of freeze-dried fruit (M. domestica, Canarium sp., and A. bilimbi) using CHOL, G6PDH, TG level, and Oil Red O assay. The viability of 3T3-L1 cell in the Canarium sp. freeze-dried in the concentration of 12.50 µg/ml has a higher value compared to M. domestica and A. bilimbi. The measurements of CHOL, G6PDH, TG level, and Oil Red O assay of the M. domestica freeze-dried in the concentration of 75 μg/ml has higher inhibitory activity compared to the Canarium sp. and A. bilimbi freeze-dried. In the CHOL assay, M. domestica freeze-dried has a higher value compared to A. bilimbi and Canarium sp. In the G6PDH assay, the freeze-dried of M. domestica has the value of 49.56%, Canarium sp. (45.22%), and A. bilimbi (47.13%), while in the Oil Red O assay, M. domestica has inhibition activity of 62.63%, A. bilimbi 50.01% and Canarium sp. 44.13%. The level of TG showed that M. domestica has higher activity with the value of 60.61%, A. bilimbi 57.54%, while Canarium sp. 55.03%. The freeze-dried of M. domestica in the concentration of 75 μg/ml has good inhibitory activity of lipid compared to A. bilimbi and Canarium sp.


Keywords


Antiobesity; adipogenesis; freeze dried; Malus domestica; Canarium sp.; Averrhhoa bilimbi.



References

Attie, A.D. and Scherer, P.E., 2009. Adipocyte metabolism and obesity. J. Lipid Res. 50, S395-S399.

Cuthbertson, D., Andrews, P.K., Reganold, J.P., Davies, N.M. & Lange, B.M., 2012. Utility of metabolomics toward assessing the metabolic basis of quality traits in apple fruit with an emphasis on antioxidants. J. Agric. Food Chem. 60(35), pp.8552-8560.

Dzomba, P. & Musekiwa, C., 2014. Antiobesity and antioxidant activity of dietary flavonoids from dioscorea steriscus tubers. J. Coastal. Life Med. 2(6),465-470.

Francini, A. & Sebastiani, L., 2013. Phenolic compounds in apple (Malus x domestica Borkh.): compounds characterization and stability during postharvest and after processing. Antioxidants. 2(3), 181-193.

Hidayat, M., Soeng, S., Prahastuti, S., Erawijantari, P.P. & Widowati, W., 2015. Inhibitory potential of ethanol extract of Detam 1 soybean (Glycine max) seed and Jati belanda (Guazuma ulmifolia) leaves on adipogenesis and obesity models in 3T3-L1 cell line. J. Sci. Res. Rep. 6(4), 305-312.

Hsu, C.L. & Yen, G.C., 2006. Induction of cell apoptosis in 3T3‐L1 pre‐adipocytes by flavonoids is associated with their antioxidant activity. Mol. Nutr. Food Res. 50(11), 1072-1079.

Hsu, C.L. &Yen, G.C., 2008. Phenolic compounds: evidence for inhibitory effects against obesity and their underlying molecular signaling mechanisms. Mol. Nutr. Food Res. 52(1), 53-61.

Jelodarian, S., Ebrahimabadi, A.H., Khalighi, A. & Batooli, H., 2012. Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area. Avicenna J Phytomed, 2(3), 139–145.

Jou, P.C., Ho, B.Y., Hsu, Y.W. & Pan, T.M., 2010. The effect of Monascus secondary polyketide metabolites, monascin and ankaflavin, on         

adipogenesis and lipolysis activity in 3T3-L1. J. Agric. Food Chem. 58(24), 12703-12709.

Kamisoyama, H., Honda, K., Tominaga, Y., Yokota, S. & Hasegawa, S., 2008. Investigation of the anti-obesity action of licorice flavonoid oil in diet-induced obese rats. Biosci. Biotechnol. Biochem. 72(12), 3225-3231.

Kim, M.J., Park, M., Jeong, M.K., Yeo, J., Cho, W.I., Chang, P.S., et al., 2010. Radical scavenging activity and anti-obesity effects in 3T3-L1 preadipocyte differentiation of Ssuk (Artemisia princeps Pamp.) extract. Food. Sci. Biotechnol. 19(2), 535-540.

Kumar, K.A., Gousia, S.K., Anupama, M. & Latha, J.N.L., 2013. A review on phytochemical constituents and biological assays of Averrhoa bilimbi. Int. J. Pharm. Pharm. Sci. Res. 3(4), 136-139.

Lahrita, L., Kato, E. & Kawabata, J., 2015. Uncovering potential of Indonesian medicinal plants on glucose uptake enhancement and lipid suppression in 3T3-L1 adipocytes. J Ethnopharmacol. 168, 229-236.

Lima, V.L.A.G.D., Mélo, E.D.A. & Lima, S., 2001. Physicochemical characteristics of bilimbi (Averrhoa bilimbi L.). Rev. Bras. Frutic. Jaboticabal. 23(2), 421-423.

Lois, K. & Kumar, S., 2009. Obesity and diabetes. Endocrinol. Nutr. 56, 38-42.

McGhie, T.K., Hunt, M. & Barnett, L.E., 2005. Cultivar and growing region determine the antioxidant polyphenolic concentration and composition of apples grown in New Zealand. J. Agric. Food Chem. 53(8), 3065-3070.

Moresco, H.H., Queiroz, G.S., Pizzolatti, M.G. & Brighente, I., 2012. Chemical constituents and evaluation of the toxic and antioxidant activities of Averrhoa carambola leaves. Rev. Bras. Farmacogn. Braz. J. Pharmacogn. 22(2), 319-324.

Park, Y.S., Yoon, Y. & Ahn, H.S., 2007. Platycodon grandiflorum extract represses up-regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats. World. J. Gastroenterol. 13(25), 3493-3499.

Pittler, M.H. and Ernst, E., 2004. Dietary supplements for body-weight reduction: a systematic review. Am. J. Clin. Nutr. 79(4), 529-536.

Roh, C., Jung, U. & Jo, S.K., 2012. Screening of anti-obesity    agent     from     herbal       mixtures. Molecules. 17(4), 3630-3638.

Sharma, S.P., Chung, H.J., Kim, H.J. and Hong, S.T., 2016. Paradoxical effects of fruit on obesity. Nutrients, 8(10), 633.

Singh, S., 2014. Effect of plant based formulations on obesity indicators in humans. Asian. J. Multidisc. Studies. 2(9),62–65.

Soeng, S., Evacuasiany, E., Widowati, W., Fauziah, N., Manik, V.T. & Maesaroh, M., 2015. Inhibitory potential of rambutan seeds extract and frac-tions on adipogenesis in 3T3-L1 cell line. J Exp Integr Med. 5, 55-60.

Swick, J.C., 2011. Effect of the flavonoid quercetin on adipocytes. Thesis. Department of Nutrition.

Utami, S., Endrini, S., Nafik, S., Lestari, I.M.T., Anindya, D., Bakar,E.A., Rozy, F., Said, F.F, Afifah, E., Arumwardana, S., Nufus, H., Rihibiha, D.D., Kusuma, H.S.W. Wibowo, S.H.B., Widowati, W.  2019. In vitro antioxidant and anti-obesity activities of freeze-dried Canarium sp., Averrhoa bilimbi L. and Malus domestica. InaBJ. 11(3):225-237

Vrhovsek, U., Rigo, A., Tonon, D. and Mattivi, F., 2004. Quantitation of polyphenols in different apple varieties. J. Agric. Food Chem. 52(21), 6532-6538.

Widowati W., Darsono, L., Suherman, J., Afifah, E., Rizal, R., Arinta, Y., Qodariah, R.L., Mozef, T., Suciati, T. 2018.  Mangosteen peel extract (Garcinia mangostana L.)  and its constituents to lower lipid content on adipogenesis cells model (3T3-L1). J Nat Remedies 18(2):41-47

Widowati W., Darsono, L., Suherman, J., Afifah, E., Rizal, R., Arinta, Y., Mozef, T., Suciati, T. 2020. Regulation of adipogenesis and key adipogenic gene expression by mangosteen pericarp extract ad xanthos in 3T3-L1. 27(1):14-21

 

 


 

 



DOI: https://doi.org/10.22146/mot.52902

Article Metrics

Abstract views : 132

Refbacks

  • There are currently no refbacks.




Copyright (c) 2021 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada