Behavioral Improvements and its Molecular Mechanism of Ilex kudingcha C.J. Tseng on Animal Model of Alzheimer’s Disease

https://doi.org/10.22146/mot.53686

Lap Thi Nguyen(1*), Nguyen Huu Son(2), Tran Nguyen Hong(3), Nguyen Minh Khoi(4), Kinzo Matsumoto(5), William R. Folk(6), Masamitsu Yamaguchi(7), Pham Thi Nguyet Hang(8)

(1) Hanoi University of Pharmacy, Hanoi
(2) 1) Hanoi University of Pharmacy, Hanoi 2) National Institute of Medcial Materials, Hanoi
(3) National Institute of Medicinal Materials, Hanoi
(4) National Institute of Medicinal Materials, Hanoi
(5) Institute of Natural Medicine, University of Toyama, Japan
(6) University of Missouri
(7) Kyoto Institute of Technology, Kyoto
(8) National Institute of Medicinal Materials, Hanoi
(*) Corresponding Author

Abstract


Alzheimer's disease (AD) is a common chronic neurodegenerative disease with well-defined pathophysiological mechanisms. Ilex kudingcha (IK) C.J. Tseng is commonly known as bitter tea or “Khom” tea in Vietnam. The present study was conducted to investigate the anti-dementia effect of IK using olfactory bulbectomized (OBX) mice. OBX mice were daily treated with IK extract (540 mg/kg) or reference drug, tacrine (2.5 mg/kg) 1 week before and continuously for 3 days after the OBX surgery. The object recognition test, modified Y maze test and fear conditioning test were employed to analyze non-spatial short-term, spatial short-term and long-term memories of the mice respectively. Administration of IK extract and tacrine attenuated these OBX-induced cognitive deficits in mice. The effects of IK and tacrine on spatial short-term memory impairment were reversed by scopolamine, a muscarinic receptor antagonist. The amyloid-beta (Aβ) production in adult transgenic Drosophila brain flies was also investigated by using Western blotting with APP-HA antibody. These results indicated that IK extract improves short-term and long-term memory disturbances in OBX mice and that muscarinic receptor may play a role on these actions. In addition, our result also showed that IK extract reduces the expression of amyloid precursor protein (APP) in brain of AD model using Drosophila melanogaster.


Keywords


Alzheimer's disease; amyloid-beta; Drosophila models; Ilex kudingcha (IK) C.J. Tseng

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References

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DOI: https://doi.org/10.22146/mot.53686

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