Effect of Ozonated Olive Oil in Topical Application towards Osteoblast Number and Angiogenesis of Alveolar Bone in Periodontitis Healing Process (in vivo study in Sprague dawley Rats)

https://doi.org/10.22146/mot.55176

Dahlia Herawati(1*), Deassy Anggraeni(2), Ariska Ratih Damayanti(3)

(1) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Ozonated olive oil has been widely used as a local infection therapy to overcome bacterial resistance from antibiotics with systemic administration. However, the disadvantage of systemic antibiotics is it cannot be used in large doses for local infection therapy. Periodontitis is an infectious disease that causes alveolar bone damage. In the periodontitis healing process, osteoblasts and angiogenesis play essential roles in bone regeneration. The study aims to determine the effect of topical application ozonated olive oil to osteoblasts number and angiogenesis of alveolar bone in periodontitis healing process using in vivo study. 32 Sprague Dawley rats were divided into two groups: the treatment and placebo groups, 16 rats in each group. The induction of periodontitis was performed by ligating lower incisor with silk ligature for seven days. The treatment group received ozonated olive oil, and the placebo group received 1 % of CMC-Na twice a day. Four rats from each group were necropsied on day 3, 5, 7, and 14 and then processed into histological sample preparations by hematoxylin-eosin staining and counted the number of osteoblast and blood vessels. All collected data were analyzed using two-way ANOVA and posthoc LSD test. The average number of osteoblasts, blood vessels in the treatment group were significantly higher than the placebo group (p <0.05) either on day 3, 5, 7, and 14. Results showed the ozonated olive oil increase the regeneration of alveolar bone in the periodontitis healing process in Sprague Dawley. Therefore ozonated olive oil has the effect of periodontal tissue regeneration.


Keywords


Periodontitis; Alveolar bone damage; Ozonated olive oil; Osteoblast; Angiogenesis

Full Text:

PDF


References

Agata, H., Asahina, I., Yamazaki, Y., Uchida, M., Shinohara, Y., Honda, M., Kagami, H., and Ueda, M. 2007. Effective Bone Engineering with Peroisteum-derived Cells. J Dental research. 86(1):79-83.

Ahmed, J., Binnal, A., Rajan, B., Denny, C., and Shenoy, N., 2013, Ozone Applications in Dentistry: An Overview, J Exp Integr Med., 3(3):171-176.

Bauer, S.M., Bauer, R.J., and Velazquez, O.C., 2005, Angiogenesis, Vasculogenesis, and Induction of Healing in Chronic Wounds, Vase Endovasc Surg, 39(4):293-306.

Baysan, A. and Beighton, D. 2007. Assessment of The Ozone-mediated Killing of Bacteria in Infected Dentine Associated with Non-cavitated Occlusal Carious Lesions. Caries Res. 41: 337-341.

Bocci, V., Borrelli, E., Travagli, V., and Zanardi, I., 2009, The Ozone Paradox: Ozone is a Strong Oxidant as Well as a Medical Drug, Medicina Research Reviews, 29(4):646-82.

Carranza, F.A., Newman F.G., and Takei, H.H. 2006. Carranza’s Clinical Periodontology. Edisi 10. Philadelphia: WB Saunders. 452-458.

Geweely, N.S.L., 2006, Antifungal Activity of Ozonated Olive Oil (Oleozone), International Journal of Agriculure & Biology, vol.8: 670-675.

Gruendermann, B.J., dan Fernsebner, B., 2005, Buku Ajar Keperawatan Perioperatif, EGC, Jakarta: 519

Gupta, G and Bansal, M. 2012. Ozone therapy in periodontics. Journal of Medicine and Life. 5 (1): 59-67.

Hardhani, P.R., Lastianny, S.P., dan Herawati, D. 2013. Pengaruh Penambahan Platelet-Rich Plasma Pada Cangkok Tulang terhadap Kadar Osteocalcin Cairan Sulkus Gingiva pada Terapi Poket Infraboni. Jurnal PDGI. 62(3): 75-82.

Indahyani, D.E. 2013. Minyak Ikan Lemuru (Sardinella longicep) Menurunkan Apoptosis Osteoblas Pada Tulang Alveolaris Tikus Wistar. Dent.J. 46(4). 185-189.

Junquiera, L.C and Carneiro, J. 2004. Histologi Dasar: Teks & Atlas (terj). Edisi 10. Jakarta: EGC. 134-156.

Kalfas, I.H. 2001. Principles of Bone Healing. Neurosurg Focus. 10(04):1-4.

Kim, H.S, Noh, S.U., Han, Y.W., Kim, K.M., Kang, H., Kim, H.O., dan Park, Y.M. 2009. Therapeutic Effects of Topical Application of Ozone on Acute Cutaneous Wound Healing. J Korean Med Sci. 24(3): 68-74.

Kotze, M., Butow, K.W., Olorunju, S.A., and Kotze, H.F., 2014, Ozone Treatment of Alveolar Bone in the Cape Chacma Baboon Does Not Enhance Healing Following Trauma, J. Maxillofac. Oral Surg., 13(2): 140-147.

Kumar, V., Ramzi S.C., dan Stanley, L.R., 2007, Buku Ajar Patologi, EGC, Jakarta:76

Laine, M.L., Crielaard, W., and Loos, B.G. 2012. Genetic Susceptibility to Periodontitis. Periodontology 2000. 58:37-68.

Li, W.W., Tsakayannis, D., and Li, V.W., 2003, Angiogenesis: A Control Point for Normal and Delayed Wound Healing, In Li, W.W., Li, V.W.,: Angiogenesis in Wound Healing, Dowden Health Media, Cambridge: 5,7-9.(3)

Meitha dan Windurini. 2003. Pengaruh Daun Lidah Buaya Terhadap Peradangan Jaringan Mukosa Rongga Mulut. JKG UI. 10 (edisi khusus): 472-7.

Mosallam, R.S., Nemat, A., El-Hoshy, A., and Suzuki, S., 2011, Effect of Oleozon on Healing of Exposed Pulp Tissues, Journal of American Science, 7(5):38-44.

Nancy, A. 2003. Ten Cate’s Oral Histology: development, structure, and function. 6thed. St.Louis: Mosby. 115-119.

Nogales, C.G., Ferrari, P.A., and Kantorovich, E.O. 2008. Ozone Therapy in Medicine and Dentistry.J Contemp Dent Pract. 9(4):75–84.

Nursal, F.K., Indriani, O., dan Dewantini, L.A., 2010, Penggunaan Na-CMC Sebagai Gelling Agent Dalam Formula Pasta Gigi Ekstrak Etanol 70% Daun Jambu Biji (Psidium guajava L), Farmasains, 1(1): 45-51.

Ozdemir, H., Toker, H., Balc, H., and Ozer, H., 2013, Effect of ozone therapy on autogenous bone graft healing in calvarial defects: a histologic and histometric study in rats, J Periodont Res., Vol 48: 722–726.

Roberts, H.C., Moseley, R., Sloan, A.J., Youde, and Waddington, R.J. 2008. Lypopolysaccharida Alters Decorin and Biglycan Synthesis in Rat Alveolar Bone Osteoblasts: Consequences for Bone Repair During Periodontal Disease. J. Oral Science. 116; 207-216.

Rowe, R,C., Sheskey P.J. dan Owen, S.C., 2006, Handbook of Pharmaceutical Excipients, Pharmaceut Press, London: 918.

Sintessa, S., Soemarko, H.M., Suprapti, L., dan Hernawan, I. 2013. Hambatan Prostaglandin pada Pemberian OAINS dan Non-OAINS Pasca Pemakaian Alat Ortodontik. J.Exp. Life Sci. 3(2): 65-75.

Srinivasan, K., Chitra, S., 2015, The Application of Ozone in Dentistry: A Systematic Review of Literature, Sch. J. Dent. Sci, 2(6):373-377.

Uchida, S., Sakai, A., Kudo, H., Otomo, H., Watanuki, M., Tanaka, M., Nagashima, M., and Nakamura, T., 2003, Vascular Endothelial Growth Factor is Expressed Along with Its Receptors during The Healing Process of Bone and Bone Marrow After Drill-Hole Injury in Rats, Bone, vol.32 issue 5: 491-501.



DOI: https://doi.org/10.22146/mot.55176

Article Metrics

Abstract views : 2420 | views : 2230

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2020 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada