Antioxidant and Anticancer Potential of Raja Bulu Banana Peel and Heart (Musa acuminata Colla (AAB group)) Ethanol Extracts in MCF-7 Cell Lines

https://doi.org/10.22146/mot.55333

Afifah Bambang Sutjiatmo(1), Wahyu Widowati(2*), Ida Sumiati(3), Twice Priestu(4), Seila Arumwardana(5), Hanna Sari Widya Kusuma(6), Alya Mardhotillah Azizah(7)

(1) Faculty of Pharmacy, Jenderal Achmad Yani University, Jl. Terusan Jenderal Sudirman, Cimahi 40531, West Java, Indonesia
(2) Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia
(3) Faculty of Pharmacy, Jenderal Achmad Yani University, Jl. Terusan Jenderal Sudirman, Cimahi 40531, West Java, Indonesia
(4) Faculty of Pharmacy, Jenderal Achmad Yani University, Jl. Terusan Jenderal Sudirman, Cimahi 40531, West Java, Indonesia
(5) Biomolecular and Biomedical Research Center Aretha Medika Utama, Jl. Babakan Jeruk II No 9, Bandung 40163, West Java, Indonesia
(6) Biomolecular and Biomedical Research Center Aretha Medika Utama, Jl. Babakan Jeruk II No 9, Bandung 40163, West Java, Indonesia
(7) Biomolecular and Biomedical Research Center Aretha Medika Utama
(*) Corresponding Author

Abstract


Breast cancer is an uncontrolled cell growth in breast tissue. Surgical treatments of breast cancer can reduce breast aesthetics and chemotherapy can cause severe side effects. It makes the searches for plants as breast anticancer agents intensively carried out. Several studies have shown that banana peels and hearts possess antioxidant and anticancer activity. This study aims to determine the fruit peel and heart of Raja Bulu banana (Musa acuminata Colla (AAB group)), an endemic banana species in Indonesia, potential as antioxidant and anticancer agent in MCF-7 cells. Antioxidant potential was determined by using 2,2-diphenyl-1picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging activity assay. Anticancer potential was determined by cytotoxic test using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The results showed that 70% ethanol extract of fruit peel (PBEE) and heart (HBEE) of Raja Bulu banana had median inhibition concentration (IC50) for DPPH scavenging activity at 115.32 µg/mL and 162.52 µg/mL respectively, while for H2O2 scavenging activity at 624.80 µg/mL and 497.13 µg/mL respectively. Anticancer potential was expressed by inhibiting concentration of 50% proliferation (IC50) of MCF-7 cells for PBEE and HBEE were 115.001 µg/mL and 338.469 µg/mL respectively. This study showed that PBEE and HBEE have antioxidant and anticancer.


Keywords


Anticancer; Antioxidant; Musa acuminate; MCF-7

Full Text:

PDF


References

Attie, A.D. and Scherer, P.E., 2009. Adipocyte metabolism and obesity. J. Lipid Res. 50, S395-S399.

Cuthbertson, D., Andrews, P.K., Reganold, J.P., Davies, N.M. & Lange, B.M., 2012. Utility of metabolomics toward assessing the metabolic basis of quality traits in apple fruit with an emphasis on antioxidants. J. Agric. Food Chem. 60(35), pp.8552-8560.

Dzomba, P. & Musekiwa, C., 2014. Antiobesity and antioxidant activity of dietary flavonoids from dioscorea steriscus tubers. J. Coastal. Life Med. 2(6),465-470.

Francini, A. & Sebastiani, L., 2013. Phenolic compounds in apple (Malus x domestica Borkh.): compounds characterization and stability during postharvest and after processing. Antioxidants. 2(3), 181-193.

Hidayat, M., Soeng, S., Prahastuti, S., Erawijantari, P.P. & Widowati, W., 2015. Inhibitory potential of ethanol extract of Detam 1 soybean (Glycine max) seed and Jati belanda (Guazuma ulmifolia) leaves on adipogenesis and obesity models in 3T3-L1 cell line. J. Sci. Res. Rep. 6(4), 305-312.

Hsu, C.L. & Yen, G.C., 2006. Induction of cell apoptosis in 3T3‐L1 pre‐adipocytes by flavonoids is associated with their antioxidant activity. Mol. Nutr. Food Res. 50(11), 1072-1079.

Hsu, C.L. &Yen, G.C., 2008. Phenolic compounds: evidence for inhibitory effects against obesity and their underlying molecular signaling mechanisms. Mol. Nutr. Food Res. 52(1), 53-61.

Jelodarian, S., Ebrahimabadi, A.H., Khalighi, A. & Batooli, H., 2012. Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area. Avicenna J Phytomed, 2(3), 139–145.

Jou, P.C., Ho, B.Y., Hsu, Y.W. & Pan, T.M., 2010. The effect of Monascus secondary polyketide metabolites, monascin and ankaflavin, on         

adipogenesis and lipolysis activity in 3T3-L1. J. Agric. Food Chem. 58(24), 12703-12709.

Kamisoyama, H., Honda, K., Tominaga, Y., Yokota, S. & Hasegawa, S., 2008. Investigation of the anti-obesity action of licorice flavonoid oil in diet-induced obese rats. Biosci. Biotechnol. Biochem. 72(12), 3225-3231.

Kim, M.J., Park, M., Jeong, M.K., Yeo, J., Cho, W.I., Chang, P.S., et al., 2010. Radical scavenging activity  and anti-obesity  effects  in   3T3-L1

preadipocyte differentiation of Ssuk (Artemisia princeps Pamp.) extract. Food. Sci. Biotechnol. 19(2), 535-540.

Kumar, K.A., Gousia, S.K., Anupama, M. & Latha, J.N.L., 2013. A review on phytochemical constituents and biological assays of Averrhoa bilimbi. Int. J. Pharm. Pharm. Sci. Res. 3(4), 136-139.

Lahrita, L., Kato, E. & Kawabata, J., 2015. Uncovering potential of Indonesian medicinal plants on glucose uptake enhancement and lipid suppression in 3T3-L1 adipocytes. J Ethnopharmacol. 168, 229-236.

Lima, V.L.A.G.D., Mélo, E.D.A. & Lima, S., 2001. Physicochemical characteristics of bilimbi (Averrhoa bilimbi L.). Rev. Bras. Frutic. Jaboticabal. 23(2), 421-423.

Lois, K. & Kumar, S., 2009. Obesity and diabetes. Endocrinol. Nutr. 56, 38-42.

McGhie, T.K., Hunt, M. & Barnett, L.E., 2005. Cultivar and growing region determine the antioxidant polyphenolic concentration and composition of apples grown in New Zealand. J. Agric. Food Chem. 53(8), 3065-3070.

Moresco, H.H., Queiroz, G.S., Pizzolatti, M.G. & Brighente, I., 2012. Chemical constituents and evaluation of the toxic and antioxidant activities of Averrhoa carambola leaves. Rev. Bras. Farmacogn. Braz. J. Pharmacogn. 22(2), 319-324.

Park, Y.S., Yoon, Y. & Ahn, H.S., 2007. Platycodon grandiflorum      extract       represses        up- regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats. World. J. Gastroenterol. 13(25), 3493-3499.

Pittler, M.H. and Ernst, E., 2004. Dietary supplements for body-weight reduction: a systematic review. Am. J. Clin. Nutr. 79(4), 529-536.

Roh, C., Jung, U. & Jo, S.K., 2012. Screening of anti-obesity    agent     from     herbal       mixtures. Molecules. 17(4), 3630-3638.

Sharma, S.P., Chung, H.J., Kim, H.J. and Hong, S.T., 2016. Paradoxical effects of fruit on obesity. Nutrients, 8(10), 633.

Singh, S., 2014. Effect of plant based formulations on obesity indicators in humans. Asian. J. Multidisc. Studies. 2(9),62–65.

Soeng, S., Evacuasiany, E., Widowati, W., Fauziah, N., Manik, V.T. & Maesaroh, M., 2015. Inhibitory potential of rambutan seeds extract  and  frac-tions   on  adipogenesis  in 3T3-L1 cell line. J Exp Integr Med. 5, 55-60.

Swick, J.C., 2011. Effect of the flavonoid quercetin on adipocytes. Thesis. Department of Nutrition.

Utami, S., Endrini, S., Nafik, S., Lestari, I.M.T., Anindya, D., Bakar,E.A., Rozy, F., Said, F.F, Afifah, E., Arumwardana, S., Nufus, H., Rihibiha, D.D., Kusuma, H.S.W. Wibowo, S.H.B., Widowati, W.  2019. In vitro antioxidant and anti-obesity activities of freeze-dried Canarium sp., Averrhoa bilimbi L. and Malus domestica. InaBJ. 11(3):225-237

Vrhovsek, U., Rigo, A., Tonon, D. and Mattivi, F., 2004. Quantitation of polyphenols in different apple varieties. J. Agric. Food Chem. 52(21), 6532-6538.

Widowati W., Darsono, L., Suherman, J., Afifah, E., Rizal, R., Arinta, Y., Qodariah, R.L., Mozef, T., Suciati, T. 2018.  Mangosteen peel extract (Garcinia mangostana L.)  and its constituents to lower lipid content on adipogenesis cells model (3T3-L1). J Nat Remedies 18(2):41-47

Widowati W., Darsono, L., Suherman, J., Afifah, E., Rizal, R., Arinta, Y., Mozef, T., Suciati, T. 2020. Regulation of adipogenesis and key adipogenic gene expression by mangosteen pericarp extract ad xanthos in 3T3-L1. 27(1):14-21

 

 


 

 



DOI: https://doi.org/10.22146/mot.55333

Article Metrics

Abstract views : 153 | views : 117

Refbacks

  • There are currently no refbacks.




Copyright (c) 2021 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada