The Effects of Green Betel Leaf (Piper betle) Extract Eye Drops on the Number of Staphylococcus aureus Colonies in Conjunctivitis Wistar Rats Model (Rattus novergicus)

Mahira Aisyah Putri Nur(1), Sudaryanto Sudaryanto(2), Endang Sri Lestari(3), Erwin Kresnoadi(4*)

(1) Faculty of Medicine, Diponegoro University, Semarang
(2) Faculty of Medicine, Diponegoro University, Semarang
(3) Faculty of Medicine, Diponegoro University, Semarang
(4) Faculty of Medicine, Diponegoro University
(*) Corresponding Author


Irrational use of antibiotics can cause resistance to some diseases such as bacterial conjunctivitis caused by Staphylococcus aureus. Previous research said that green betel leaf (Piper betle) contains antimicrobial compounds such as eugenol, cavalry, tannins, saponins, and flavonoids which also have antiseptic power as well as antibiotics. This study aims to prove the decrease in the number of Staphylococcus aureus colonies after giving green betel leaf eye drops (Piper betle) to conjunctivitis Wistar rats (Rattus norvegicus). This research was true experimental with a pretest-posttest control group design. 25 male white Wistar rats as objects were selected by simple random sampling and grouped into 5 groups randomly. The rats were given Staphylococcus aureus, control group K+ was given 0.5% levofloxacin eye drops, and K- was given aquadest as a comparison. Treatment groups P1, P2, and P3 were given different concentrations of betel leaf eye drops. Swab pretest was done 3 days after the rats were inoculated with Staphylococcus aureus and swab post-test was done 5 days after the rats were given green betel leaf eye drops, the calculation of Staphylococcus aureus bacteria colonies used the Total Plate Count (TPC) method. There was a significant decrease (p<0.05) in the number of bacterial colonies for K-, K+, P1, P2, and P3 groups. In addition, there was a significant difference (p<0.05) between K+ with P1 and K+ with P3. There was a decrease in the number of Staphylococcus aureus for all groups of conjunctivitis Wistar rats (Rattus norvegicus)


Bacterial conjunctivitis, Staphylococcus aureus, Piper betle, Total Plate Count

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Afjeiee SA, Tabatabaei SR, Fallah F, Fard AT, Shiva F, Adabian S, et al. 2013. A Microbiological Study of Neonatal Conjunctivitis in Two Hospitals in Tehran, Iran. Asian Pacific J Trop Dis.

Devi KP, Nisha SA, Sakthivel R, Pandian SK. 2012. Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. Journal of Ethnopharmacology. 2010; 130(1) 107-115.

Epling J. Bacterial conjunctivitis. 2010. BMJ clinical evidence. BMJ clinical evidence.

Haq A, Wardak H, Kraski N. 2013. Infective Conjunctivitis – Its Pathogenesis, Management and Complications [Internet]. Intechopen. [citied 2020 May 20]. Available from:

Ilyas S, Yulianti SR. 2017. Ilmu Penyakit Mata. Jakarta : Fakultas Kedokteran Universitas Indonesia.

Inayatullah S. Efek Ekstrak Daun Sirih Hijau (Piper betle L.) terhadap Pertumbuhan Bakteri Staphylococcus aureus [skripsi]. Jakarta: Fakultas Kedokteran dan Ilmu Kesehatan UIN Syarif Hidayatullah.

Kemenkes RI 2010. 2010. Profil Kesehatan Indonesia 2009. Jakarta : Pusat Data dan Informasi Profil Kesehatan Indonesia.

Putri ZF. 2010. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirih (Piper betle L.) terhadap Propionibacterium acne dan Staphylococcus aureus Multiresisten. Surakarta: Fakultas Farmasi Universitas Muhammadiyah Surakarta.

Sanjaya GW. 2018. Efek Ekstrak Daun Murbei (Morus Alba L.) Pada Tikus Wistar Model Konjungtivitis Oleh Staphylococcus aureus [skripsi]. Jember: Fakultas Kedokteran Universitas Jember.

Vaughan, D dan TA. 2016. Vaughan & Asbury Ophtalmology Umum. Jakarta : Buku Kedokteran EGC.

Warsa U. 2011. Buku ajar Mikrobiologi Kedokteran : Kokus Gram Positif Staphylococcus. Jakarta: Binarupa Aksara.


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