.png)
.png)
* Corresponding Author
Supplementation of Garcinia mangostana Linn and Vasconcellea pubescens A.DC extract reduced exercise-induced oxidative stress in rats
https://doi.org/10.22146/mot.68517 Heru Sasongko(1*), Sugiyarto Sugiyarto(2), Renita Wahyu Nur Hidayati(3), Bayu Anggoro Saputro(4)
(1) Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57 126, Jawa Tengah
(2) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret Jl. Ir. Sutami No. 36 A, Surakarta, 57126
(3) Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A, Surakarta, 57126
(4) Department of Pharmacy, Universitas Sebelas Maret Jl. Ir. Sutami No. 36 A, Surakarta, Indonesia, 57126
(*) Corresponding Author
Abstract
The objective of this study was to see how supplementation with Garcinia mangostana extract (GME) and Vasconcellea pubescens extract (VPE) affected exercise performance and oxidative stress in intensely exercised rats. Twenty-five male rats were used in the study. Rats were divided into five groups: normal, stress oxidative control, combination GME:VPE (50:50), combination GME:VPE (75:25), and combination GME:VPE (25:75) groups. The antioxidant activity of a single extract from the literature was used to make the percentage comparison between extracts. The chronic exercise was done by swimming at 10 m/day for two weeks. Rats in the acute exercise groups were treated by swimming on the pail containing water. Blood samples were collected from the orbital sinus to determine hematological parameters. Liver tissue samples for the analysis of malondialdehyde (MDA) and glutathione (GPx) markers. Data analysis was performed statistically using One Way ANOVA. The combination of GME and VPE was shown to be effective in reducing oxidative stress and increasing MDA and GPx enzyme activity. The administration of both extracts also showed changes in the hematological profile.
Keywords
Full Text:
PDFReferences
Abdel-Moneim, A., Mahmoud, B., Nabil, A., Negeem, Z., 2019a. Correlation between oxidative stress and hematological profile abnormalities in diabetic nephropathy. Diabetes & Metabolic Syndrome: Clinical Research & Reviews 13, 2365–2373. https://doi.org/10.1016/j.dsx.2019.06.014
Abdel-Moneim, A., Zanaty, M.I., El-Sayed, A., Khaled, R.G., Rahman, H.A., 2019b. Relation between oxidative stress and hematological abnormalities in children with type 1 diabetes. Canadian Journal of Diabetes. https://doi.org/10.1016/j.jcjd.2019.07.153
Arsana, I., Adiputra, N., Pangkahila, J., Putra-Manuaba, I., 2013. Garcinia mangostana L. rind extract and physical training reduce oxidative stress in Wistar rats during maximal physical activity. Indonesian Journal of Biomedical Science 7, 63–68.
Asmat, U., Abad, K., Ismail, K., 2016. Diabetes mellitus and oxidative stress—A concise review. Saudi Pharmaceutical Journal 24, 547–553. https://doi.org/10.1016/j.jsps.2015.03.013
Belviranlı, M., Gökbel, H., Okudan, N., Başaralı, K., 2012. Effects of grape seed extract supplementation on exercise-induced oxidative stress in rats. British Journal of Nutrition 108, 249–256. https://doi.org/10.1017/S0007114511005496
Betteridge, D.J., 2000. What is oxidative stress? Metabolism, Advances in Oxidative Stress Proceedings of an “Expert Session” held on the Occasion of the Annual Meeting of the European Association for the study of Diabetes 49, 3–8. https://doi.org/10.1016/S0026-0495(00)80077-3
Boskabady, M.H., Farhadi, J., 2008. The possible prophylactic effect of Nigella sativa seed aqueous extract on respiratory symptoms and pulmonary function tests on chemical war victims: a randomized, double-blind, placebo-controlled trial. The Journal of Alternative and Complementary Medicine 14, 1137–1144.
Chang, C.-C., Chen, C.-W., Owaga, E., Lee, W.-T., Liu, T.-N., Hsieh, R.-H., 2020. Mangosteen Concentrate Drink Supplementation Promotes Antioxidant Status and Lactate Clearance in Rats after Exercise. Nutrients 12, 1447. https://doi.org/10.3390/nu12051447
Chen, C.-Y., Kao, C.-L., Liu, C.-M., 2018. The Cancer Prevention, Anti-Inflammatory and Anti-Oxidation of Bioactive Phytochemicals Targeting the TLR4 Signaling Pathway. International Journal of Molecular Sciences 19, 2729. https://doi.org/10.3390/ijms19092729
Davies, K.J., Quintanilha, A.T., Brooks, G.A., Packer, L., 1982. Free radicals and tissue damage produced by exercise. Biochemical and biophysical research communications 107, 1198–1205.
Duclos, M., Gouarne, C., Bonnemaison, D., 2003. Acute and chronic effects of exercise on tissue sensitivity to glucocorticoids. Journal of applied physiology 94, 869–875.
e Silva, T.S., Longui, C.A., Rocha, M.N., Faria, C.D.C., Melo, M.R., Faria, T.G., De Souza, J.A., Rizzo, L.V., 2010. Prolonged physical training decreases mRNA levels of glucocorticoid receptor and inflammatory genes. Hormone research in paediatrics 74, 6–14.
Ellman, G.L., 1959. Tissue sulfhydryl groups. Archives of biochemistry and biophysics 82, 70–77.
Grażyna, C., Hanna, C., Adam, A., Magdalena, B.M., 2017. Natural antioxidants in milk and dairy products. International Journal of Dairy Technology 70, 165–178. https://doi.org/10.1111/1471-0307.12359
Guder, W.G., Narayanan, S., Wisser, H., Zawta, B., 2014. Diagnostic Samples: From the Patient to the Laboratory: The Impact of Preanalytical Variables on the Quality of Laboratory Results. John Wiley & Sons.
Hassan, W., Zulkifli, R.M., Basar, N., Ahmad, F., Yunus, M.A.C., 2015. Antioxidant and tyrosinase inhibition activities of α-mangostin and Garcinia mangostana Linn. pericarp extracts. Journal of Applied Pharmaceutical Science 5, 037–040.
Hellsten, Y., Frandsen, U., Orthenblad, N., Sjødin, B., Richter, E., 1997. Xanthine oxidase in human skeletal muscle following eccentric exercise: a role in inflammation. The Journal of physiology 498, 239–248.
Husain, K., 2003. Interaction of exercise training and chronic NOS inhibition on blood pressure, heart rate, NO and antioxidants in plasma of rats. Pathophysiology 10, 47–56.
Im, A.-R., Im, A.-R., Kim, Y.-M., Kim, Y.-M., Chin, Y.-W., Chin, Y.-W., Chae, S., Chae, S., 2017. Protective effects of compounds from Garcinia mangostana L. (mangosteen) against UVB damage in HaCaT cells and hairless mice. International Journal of Molecular Medicine 40, 1941–1949. https://doi.org/10.3892/ijmm.2017.3188
Jackson, M.J., Vasilaki, A., McArdle, A., 2016. Cellular mechanisms underlying oxidative stress in human exercise. Free Radical Biology and Medicine, Human Performance and Redox Signaling in Health and Disease 98, 13–17. https://doi.org/10.1016/j.freeradbiomed.2016.02.023
Jiménez-Aguilar, D.M., Grusak, M.A., 2017. Minerals, vitamin C, phenolics, flavonoids and antioxidant activity of Amaranthus leafy vegetables. Journal of Food Composition and Analysis 58, 33–39. https://doi.org/10.1016/j.jfca.2017.01.005
Johannsen, N.M., Swift, D.L., Johnson, W.D., Dixit, V.D., Earnest, C.P., Blair, S.N., Church, T.S., 2012. Effect of different doses of aerobic exercise on total white blood cell (WBC) and WBC subfraction number in postmenopausal women: results from DREW. PloS one 7, e31319.
Laily, A.N., Suranto, S., Sugiyarto, S., 2012. Characterization of Carica pubescens in Dieng Plateau, Central Java based on morphological characters, antioxidant capacity, and protein banding pattern. Nusantara Bioscience 4.
Mai, A.S., dos Santos, A.B., Beber, L.C.C., Basso, R.D.B., Sulzbacher, L.M., Goettems-Fiorin, P.B., Frizzo, M.N., Rhoden, C.R., Ludwig, M.S., Heck, T.G., 2017. Exercise Training under Exposure to Low Levels of Fine Particulate Matter: Effects on Heart Oxidative Stress and Extra-to-Intracellular HSP70 Ratio. Oxidative Medicine and Cellular Longevity 2017, e9067875. https://doi.org/10.1155/2017/9067875
Nurrochmad, A., Margono, S.A., Sardjiman, Hakim, A.R., Ernawati, Kurniawati, E., Fatmawati, E., 2013. Hepatoprotective and antioxidant activity of pentagamavunon-0 against carbon tetrachloride-induced hepatic injury in rats. Asian Pacific Journal of Tropical Medicine 6, 438–442. https://doi.org/10.1016/S1995-7645(13)60070-X
Olale, K., Walyambillah, W., Mohammed, S.A., Sila, A., Shepherd, K., 2019. FTIR-DRIFTS-based prediction of β-carotene, α-tocopherol and l-ascorbic acid in mango (Mangifera indica L.) fruit pulp. SN Appl. Sci. 1, 279. https://doi.org/10.1007/s42452-019-0297-7
Osorio, R., Christofani, J., D’almeida, V., Russo, A., Piçarro, I. da C., 2003. Reactive oxygen species in pregnant rats: effects of exercise and thermal stress. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 135, 89–95.
Rasoanaivo, P., Wright, C.W., Willcox, M.L., Gilbert, B., 2011. Whole plant extracts versus single compounds for the treatment of malaria: synergy and positive interactions. Malar J 10, S4. https://doi.org/10.1186/1475-2875-10-S1-S4
Revan, S., Erol, A.E., 2011. Effects of endurance training on exhaustive exercise-induced oxidative stress markers. African Journal of Pharmacy and Pharmacology 5, 437–441.
Ribeiro-Samora, G.A., Rabelo, L.A., Ferreira, A.C.C., Favero, M., Guedes, G.S., Pereira, L.S.M., Parreira, V.F., Britto, R.R., Ribeiro-Samora, G.A., Rabelo, L.A., Ferreira, A.C.C., Favero, M., Guedes, G.S., Pereira, L.S.M., Parreira, V.F., Britto, R.R., 2017. Inflammation and oxidative stress in heart failure: effects of exercise intensity and duration. Brazilian Journal of Medical and Biological Research 50. https://doi.org/10.1590/1414-431x20176393
Rush, J.W.E., Denniss, S.G., Graham, D.A., 2005. Vascular Nitric Oxide and Oxidative Stress: Determinants of Endothelial Adaptations to Cardiovascular Disease and to Physical Activity. Can. J. Appl. Physiol. 30, 442–474. https://doi.org/10.1139/h05-133
Sakr, S.A., Al-Amoudi, W.M., 2012. Effect of leave extract of Ocimum basilicum on deltamethrin induced nephrotoxicity and oxidative stress in albino rats. Journal of Applied Pharmaceutical Science 2, 22.
Sasongko, H., Efendi, N.R., Sugiyarto, 2018. The ethanolic extract of mountain papaya (Vasconcellea pubescens A.DC.) fruit against lipid peroxidation of rat liver tissues. AIP Conference Proceedings 2019, 050001. https://doi.org/10.1063/1.5061894
Sasongko, H., Lestari, R., Yugatama, A., Farida, Y., Farida, Y., Farida, Y., 2020. Antidiabetic and Antioxidant Effect Combination Vasconcellea pubescens A.DC. and Momordica charantia L. Extract in Alloxan- Induced Diabetic Rats. Pharmacognosy Journal 12, 311–315. https://doi.org/10.5530/pj.2020.12.49
Sasongko, H., Pratiwi, D., Amartiwi, T., Efendi, N.R., Sugiyarto, 2019. Hepatoprotective Effect of Mountain Papaya (Vasconcellea pubescens A.DC.) Fruit Extract against Acetaminophen-Induced Acute Liver Damage. Presented at the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development, pp. 66–70.
Sinaga, R.N., Zulaini, Z., 2020. The Effect of Mangosteen Rind Extract (Garcinia mangostana L.) Toward Stress Oxidative Parameter, Leukocytes, Leukocytes Type Counts on Male Rats (Rattus norvegicus) with Excessive Physical Activity. Open Access Macedonian Journal of Medical Sciences 8, 904–909.
Thomford, N.E., Senthebane, D.A., Rowe, A., Munro, D., Seele, P., Maroyi, A., Dzobo, K., 2018. Natural Products for Drug Discovery in the 21st Century: Innovations for Novel Drug Discovery. Int J Mol Sci 19, 1578. https://doi.org/10.3390/ijms19061578
Tsikas, D., 2017. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Analytical Biochemistry, Lipid Methodology 524, 13–30. https://doi.org/10.1016/j.ab.2016.10.021
Viña, J., Gimeno, A., Sastre, J., Desco, C., Asensi, M., Pallardó, F.V., Cuesta, A., Ferrero, J.A., Terada, L.S., Repine, J.E., 2000. Mechanism of free radical production in exhaustive exercise in humans and rats; role of xanthine oxidase and protection by allopurinol. IUBMB life 49, 539–544.
Zheng, X., Long, W., Liu, G., Zhang, X., Yang, X., 2012. Effect of seabuckthorn (Hippophae rhamnoides ssp. sinensis) leaf extract on the swimming endurance and exhaustive exercise‐induced oxidative stress of rats. Journal of the Science of Food and Agriculture 92, 736–742.
DOI: https://doi.org/10.22146/mot.68517
Article Metrics
Abstract views : 2226
| 
views : 1473
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada