Antioxidant Activity of TEMON (Clitoria ternatea and Citrus sp.) as an Infused Herbal Tea

https://doi.org/10.22146/mot.71628

Wahyu Widowati(1*), Teresa Liliana Wargasetia(2), Teddy Markus Zakaria(3), Meganita Marthania(4), Ria Aprilia Tri Puteri Permata Akbar(5), Michael Sebastian Gunadi(6), Nathanael Halim(7), Sherly Santiadi(8)

(1) Faculty of Medicine, Maranatha Christian University, Bandung, West Java
(2) Faculty of Medicine, Maranatha Christian University, Bandung, West Java
(3) Faculty of Information Technology, Maranatha Christian University, Bandung, West Java
(4) Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, West Java
(5) Faculty of Mathematics and Natural Sciences Education, Indonesian Education University, Bandung, West Java
(6) Faculty of Information Technology, Maranatha Christian University, Bandung, West Java
(7) Faculty of Information Technology, Maranatha Christian University, Bandung, West Java
(8) Faculty of Information Technology, Maranatha Christian University, Bandung, West Java
(*) Corresponding Author

Abstract


Many people around the world believed that herbal tea had a lot of antioxidants contained in it, thus it was widely drunk all over the world. However, the antioxidant capacities of herbal tea may be various because it is affected by the usage of different plants as its primary ingredient. As a result, this study examines the antioxidant activity of herbal tea made from dried Citrus sp. and Clitoria ternatea flower petals. The antioxidant potential was assessed by performing a DPPH and H2O2 scavenging activity assay on 2,2-diphenyl-1picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging activity assay, total phenolic, and flavonoid content assay, ABTS reduction, and FRAP assay. This study showed that pure telang (Clitoria ternatea) herbal tea has antioxidant properties. Dried Telang (Clitoria ternatea) tea had the highest activity of antioxidants as we refer to the results of it had the highest activity value in DPPH, ABTS, and FRAP assay and also had the lowest IC-50 value. Followed by TEMON infusion which showed the second-highest antioxidant activity after pure telang infusion. The decrease in antioxidant activity in the mixture of telang and lemon was due to the mixture of lemons which tended to have the lowest antioxidant activity among the three types of infusions tested. The addition of dried lemon has the aim of enhancing the flavor of the tea infusion.

Keywords


Antioxidants; Herbal Tea; Citrus sp.; Clitoria ternatea; Flavonoids

Full Text:

PDF


References

Bravo, L., 1998, ‘Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance’, Nutrition reviews, 56(11), 317-333.

Cai, Y., Luo, Q., Sun, M., & Corke, H., 2004, ‘Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer’, Life sciences, 74(17), 2157-2184.

Devi, D. P., Boominathan, R., & Mandal, S. C., 2003, ‘Anti-inflammatory, analgesic and antipyretic properties of Clitoria ternatea root’, Fitoterapia, 74, 345–349.

Gomez, S. M., & Kalamani, A., 2003, ‘Butterfly pea (Clitoria ternata): A nutritive multipurpose forage legume for the tropics – an overview’, Pak J Nutr. 2, 374–379.

Hall, T. J., 1992, ‘Clitoria ternata L. (butterfly pea) cv. Milgarra’, Aust J Exp Agric. 32, 547–548.

Jain, N. N., Ohal, C. C., & Shroff, S. K., 2003, ‘Clitoria ternatea and the CNS’, Pharmacol Biochem Behav, 75, 529–536.

Kazuma, K., Noda, N., & Suzuki, M., 2003, ‘Flavonoid composition related to petal color in different lines of Clitoria ternatea’, Phytochemistry, 64, 1133–1139.

Ke, Z. L., Pan, Y., Xu, X. D., Nie, C., & Zhou, Z. Q., 2015, ‘Citrus Flavonoids and Human Cancers’, Journal of Food and Nutrition Research, 3(5), 341-351.

Kedare, S. B., & Singh, R. P., 2011, ‘Genesis and development of DPPH method of antioxidant assay’, Journal of Food Science and Technology, 48, 412–422.

Mehl, F., Marti, G., Boccard, J., Debrus, B., Merle, P., Delort, E., Baroux, L., Raymo, V., Velazco, M. I., Sommer, H., Wolfender, J., & Rudaz, S., 2014, ‘Differentiation of lemon essential oil based on volatile and non-volatile fractions with various analytical techniques: a metabolomic approach’, Food chemistry, 143, 325-335.

Mukhopadhyay, D., Dasgupta, P., Roy, D. S., Palchoudhuri, S., Chatterjee, I., Ali, S., & Dastidar, S.G., 2016, ‘A sensitive in vitro spectrophotometric hydrogen peroxide scavenging assay using 1, 10-phenanthroline’, Free Radicals & Antioxidants, 6(1), 123-131.

Nurhayati, B., Rahayu, I. G., Rinaldi, S. F., Zaini, W. S., Afifah, E., Arumwardana, S., Kusuma, H. S. W., Rizal, R. & Widowati, W., 2018, ‘The antioxidant and cytotoxic effects of Cosmos caudatus ethanolic extract on cervical cancer’, The Indonesian Biomedical Journal, 10(3), 243-249.

Packer, L., Cadenas, E. & Davies, K.J., 2008, ‘Free radicals and exercise: an introduction’, Free radical biology & medicine, 44(2), 123-125.

Prahastuti, S., Hidayat, M., Hasiana, S. T., Widowati, W., Amalia, A., Qodariah, R. L., Rizal, R., Kusuma, H. S. W., & Khoiriyah, Z., 2019a, ‘Ethanol extract of jati belanda (Guazuma ulmifolia L.) as Therapy for Chronic Kidney Disease in In Vitro model’, Journal of Reports in Pharmaceutical Sciences, 8(2), 229.

Prahastuti, S., Hidayat, M., Hasianna, S.T., Widowati, W., Amalia, A., Yusepany, D.T., Rizal, R. & Kusuma, H.S.W., 2019b, ‘Antioxidant potential ethanolic extract of Glycine max (l.) Merr. Var. Detam and daidzein’, Journal of Physics: Conference Series, 1374, 1-12.

Quispe, C., Viveros-Valdez, E. & Schmeda-Hirschmann, G., 2012, ‘Phenolic constituents of the Chilean herbal tea Fabiana imbricata R. et P’, Plant Foods for Human Nutrition, 67(3), 242–6.

Rajendran, P., Nandakumar, N., Rengarajan, T., Palaniswami, R., Gnanadhas, E. N., Lakshminarasaiah, U., Gopas J., & Nishigaki, I., 2014, ‘Antioxidants and human diseases’, Clinica Chimica Acta, 436, 332-347.

Rusmana, D., Wahyudianingsih, R., Elisabeth, M., Balqis, B., Maesaroh, M. & Widowati, W., 2017, ‘Antioxidant activity of Phyllanthus niruri extract, rutin and quercetin’, The Indonesian Biomedical Journal, 9(2), 84-90.

Sasikumar, V., & Kalaisezhiyen, P., 2014, ‘Evaluation of free radical scavenging activity of various leaf extracts from Kedrostis foetidissima (Jacq.) Cogn’, Biochemistry and Analytical Biochemistry, 3(2), 1.

Terahara, N., Oda, M., Matsui, T., Osajima, Y., Saito, N., Toki, K., & Honda, T., 1996, ‘Five new anthocyanins, ternatins A3, B4, B3, B2, and D2, from Clitoria ternatea flowers’, J Nat Prod, 59, 139–144.

Tschiggerl, C. & Bucar, F., 2012, ‘The volatile fraction of herbal teas’, Phytochemistry Reviews, 11 (2-3), 245- 254.

Widowati, W., Fauziah, N., Herdiman, H., Afni, M., Afifah, E., Kusuma, H. S. W., Nufus, H., Arumwardana, S. & Rihibiha, D. D., 2016, ‘Antioxidant and Anti-aging Assays of Oryza sativa Extracts, Vanillin and Coumaric Acid’, Journal of Natural Remedies, 16(3), 88-99.

Widowati, W., Herlina, T., Ratnawati, H., Constantia, G., Deva, I. D. G. S., & Maesaroh, M., 2015, ‘Antioxidant potential of black, green and oolong tea methanol extracts’, Biology, Medicine, & Natural Product Chemistry, 4(2), 35-39.

Widowati, W., Janeva, B. W., Nadya, S., Amalia, A., Arumwardana, S., Kusuma, H. S. W. & Arinta, Y., 2018, ‘Antioxidant and antiaging Antiaging activities Activities of Jasminum sambac Extract, and its Compounds’, Journal of Reports in Pharmaceutical Sciences, 7(3), 270.

Widowati, W., Rani, A. P., Hamzah, R. A., Arumwardana, S., Afifah, E., Kusuma, H. S. W., Rihibiha, D. D., Nufus, H., & Amalia, A., 2017, ‘Antioxidant and antiaging assays of Hibiscus sabdariffa Extract and its Compounds’, Natural Product Sciences, 23(3), 192- 200.

Yu, J., Wang, L., Walzem, R. L., Miller, E. G., Pike, L. M., & Patil, B. S., 2005, ‘Antioxidant activity of citrus limonoids, flavonoids, and coumarins’, Journal of agricultural and food chemistry, 53(6), 2009-2014.

Zhao, J., Deng, J. W., Chen, Y. W., & Li, S. P., 2013, ‘Advanced phytochemical analysis of herbal tea in China’, Journal of Chromatography A. 1313, 2-23.



DOI: https://doi.org/10.22146/mot.71628

Article Metrics

Abstract views : 4826 | views : 3025

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2022 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada