.png)
.png)
* Corresponding Author
The Effect of Cultivation Site Altitudes on the Quality Parameters of Meniran (Phylanthus niruri L.)
https://doi.org/10.22146/mot.72163 Dinar Sari C. Wahyuni(1*), Maya Widia Pangestika(2), Della Andriyani(3), Yudi Rinanto(4), Siti Fatimah(5)
(1) Pharmacy Department, Faculty of Mathematics and Natural Science, Universitas Sebelas Maret, Surakarta
(2) Pharmacy Department, Faculty of Mathematics and Natural Science, Universitas Sebelas Maret, Surakarta
(3) Pharmacy Department, Faculty of Mathematics and Natural Science, Universitas Sebelas Maret, Surakarta
(4) Biology Education, Faculty of Teacher Training and Education, Universitas Sebelas Maret, Surakarta
(5) Indonesian Ministry of Health, Central Java
(*) Corresponding Author
Abstract
Meniran (Phyllanthus niruri L.) is known as a medicinal plant for immunomodulatory, hypoglycemic, diuretic treatment, and kidney disorders. It has phenolic and flavonoid as the active compounds. Several commercial products utilize meniran as the main ingredient for immunomodulators. However, the standard raw material for production has not been well reported. The study aimed to standardize the quality of meniran’s raw materials from different locations and altitudes. The phenolic compounds as the active component and their antioxidant activity were further studied. The raw materials were collected from Sukoharjo, Jember, and Karanganyar which are located from 104 masl, 243 masl, and 722 masl. The specific and non-specific standardization based on Indonesian Herbal Pharmacopoeia were investigated. The antioxidant activity was investigated. It resulted in the specific and non-specific parameters that were according to standard except for the acid soluble ash test from the Jember sample which was much higher (6.333% ± 0.969) than the standard (<1.2%). In addition, the flavonoid (1.529 ± 0.167%w/w) and phenolic (1.65 ± 0.006 %w/w) content showed that the sample collected from Jember was significantly higher than other locations. However, the strongest antioxidant activity was in the sample collected from Sukoharjo (IC50 101.84μg/mL). On the other hand, there was no correlation between the total flavonoid and the total phenolic to antioxidant activity in this plant. It suggested that the antioxidant activity in these plants did not depend on the flavonoid and phenolic compounds. In conclusion, the raw material of meniran (Phyllanthus niruri L.) collected from 3 different altitudes showed the quality of herbal medicine of mainly non-specific parameters and met the requirements of Indonesian Herbal Pharmacopoeia. The total phenolic and flavonoid as the active substances varied through all 3 different altitudes. It was in line with the antioxidant activity which varied to all 3 different altitudes due to the phytochemical profile variety.
Keywords
Full Text:
PDFReferences
Aldi, Y., Rasyadi, Y. and Handayani, D. (2014) ‘Aktivitas Imunomodulator dari Ekstrak Etanol Meniran (Phyllanthus niruri Linn.) terhadap Ayam Broiler’, Jurnal Sains Farmasi & Klinis, 1(1), pp. 20–26.
Alegantina, S., Setyorini, H. A. and Triwahyuni, T. (2015) ‘Pengujian Mutu Dan Penetapan Kadar Filantin Pada Ekstrak Etanol Herba Meniran (Phyllanthus Niruri Linn)’, Buletin Penelitian Kesehatan, 43(1), pp. 11–16.
Benítez, V. et al. (2011) ‘Study of bioactive compound content in different onion sections’, Plant Foods for Human Nutrition, 66(1), pp. 48–57.
Chairul, Jamal, Y. and Zainul, Z. (2000) ‘Efek Hipoglikemik Ekstrak Alkohol Herba Meniran (Phyllanthus niruri L.) Pada Kelinci Putih Jantan’, Berita Biologi, 5(April), pp. 93–101.
Dewi, S. R., Argo, B. D. and Ulya, N. (2018) ‘Kandungan Flavonoid dan Aktivitas Antioksidan Ekstrak Pleurotus ostreatus’, Rona Teknik Pertanian, 11(1), pp. 1–10. doi: 10.17969/rtp.v11i1.9571.
Fariz, A. et al. (2018) ‘Review: Tanaman Obat yang Berefek Sebagai Antigout’, Jurnal Pharmascience, 5(1), pp. 22–31.
Gunawan, I. W. G., Gede Bawa, I. G. A. and Sutrisnayanti, N. L. (2008) ‘Isolasi Dan Identifikasi Senyawa Terpenoid Yang Aktif Antibakteri Pada Herba Meniran (Phyllanthus niruri Linn)’, Jurnal Kimia, 2(1), pp. 31–39.
Hajian, S. (2014) ‘Positive effect of antioxidants on immune system’, Immunopathologia Persa, 1(1), p. e02.
Ifandari, Suranto and Wuryaningsih, Y. N. S. (2013) ‘Efek Perlakuan Ekstrak Meniran Merah (Phyllanthus urinaria) Terhadap Jumlah Limfosit Pada Organ Limpa Mencit Balb/C Yang Diinfeksi Bakteri Salmonella Thypi’, Biomedika, 6(2), pp. 1–6.
Jansen, I., Wuisan, J. and Awaloei, H. (2015) ‘Uji Efek Antipiretik Ekstrak Meniran (Phyllantus niruri L.) Pada Tikus Wistar (Rattus norvegicus) Jantan Yang Diinduksi Vaksin Dpt-Hb’, Jurnal e-Biomedik, 3(1), pp. 3–7.
Jugran, A. K. et al. (2016) ‘Impact of altitudes and habitats on valerenic acid, total phenolics, flavonoids, tannins, and antioxidant activity of Valeriana jatamansi’, Applied biochemistry and biotechnology, 179, pp. 911–926.
Krisyanella, Susilawati, N. and Rivai, H. (2013) ‘Pembuatan dan karakterisasi serta penentuan kadar flavonoid dari ekstrak kering herba meniran (Phyllanthus niruri L.)’, Jurnal Farmasi Higea, 5(1), pp. 9–19.
De la Fuente, M. (2002) ‘Effects of antioxidants on immune system ageing’, European Journal of Clinical Nutrition, 56(3), pp. S5–S8.
Markham, K. R. (1988) Cara Mengidentifikasi Flavonoid. Edited by D. K. Padmawinata. Bandung: ITB Bandung.
Pandey, G. et al. (2018) ‘Altitudinal variation of berberine, total phenolics and flavonoid content in Thalictrum foliolosum and their correlation with antimicrobial and antioxidant activities’, Journal of Ayurveda and integrative medicine, 9(3), pp. 169–176.
Paramita, N. L. P. V. et al. (2019) ‘Karakteristik Simplisia Teh Hitam Dari Tanaman Camelia sinensis Var. assamica Dari Perkebunan Teh Bali Cahaya Amerta, Desa Angseri, Kecamatan Baturiti, Kabupaten Tabanan, Bali’, Jurnal Kimia, 13(1), pp. 58–66. doi: 10.24843/jchem.2019.v13.i01.p10.
Priti et al. (2021) ‘Diversity in phytochemical composition, antioxidant capacities, and nutrient contents among mungbean and lentil microgreens when grown at plain-altitude region (Delhi) and high-altitude region (Leh-Ladakh), India’, Frontiers in Plant Science, 12, p. 710812.
PUTRI, R. (2018) ANALISIS KUALITATIF DAN PENETAPAN KADAR FENOL EKSTRAK HEKSANA, ASETON, METANOL DAN AIR DARI MENIRAN (Phyllanthus niruri L.), Tugas Akhir. Universitas Andalas.
Rakhmawati, N., Amanto, B. S. and Praseptiaga, D. (2014) ‘Formulasi Dan Evaluasi Sifat Sensor Dan Fisiokimia Produk Flakes Komposit Berbahan Dasar Tepung Tapioka, Tepung Kacang Merah (phaseolus vulgaris L.) Dan Tepung Konjac (Amorphophallus oncophillus)’, Jurnal Teknosains Pangan, 3(1), pp. 63–73.
Rosadi, I. and Marini (2016) ‘Perbandingan Daya Diuretik Infus Herba Meniran Hijau (Phyllanthus niruri L.) Dengan Furosemid Pada Kelinci Jantan (Ocytagus caniculus)’, Jurnal Farmaku, 1(2), pp. 30–40.
Safrina, D. et al. (2018) ‘Pengaruh Ketinggian Tempat Tumbuh Dan Pengeringan Terhadap Flavonoid Total Sambang Colok (Iresine herbstii)’, Jurnal Penelitian Pascapanen Pertanian, 15(3), pp. 147–154.
Sagar, N. A., Pareek, S. and Gonzalez-Aguilar, G. A. (2020) ‘Quantification of flavonoids, total phenols and antioxidant properties of onion skin: A comparative study of fifteen Indian cultivars’, Journal of food science and technology, 57, pp. 2423–2432.
Sandrasari, D. A. (2011) ‘Aktivitas Antioksidan Ekstrak Daun Meniran’, TEKNOTAN, 5(3).
Sun, T., Powers, J. R. and Tang, J. (2007) ‘Evaluation of the antioxidant activity of asparagus, broccoli and their juices’, Food chemistry, 105(1), pp. 101–106.
Susanty, Bachdim, F. (2016) ‘Perbandingan Metode Ekstraksi Maserasi dan Refluks terhadap Kadar Fenolik dari Ekstrak Tongkol Jagung (Zea mays L.)’, Konversi, 5(2), pp. 87–93.
Tambunan, R. M., Swandiny, G. F. and Zaidan, S. (2019) ‘Uji Aktivitas Antioksidan dari Ekstrak Etanol 70% Herba Meniran (Phyllanthus niruri L.) Terstandar’, Sainstech Farma: Jurnal Ilmu Kefarmasian, 12(2), pp. 60–64.
Winarti, W., Nuryanti, S. and Said, I. (2014) ‘Pengaruh Konsentrasi Ekstrak Tanaman Meniran (Phillanthus Niruri L.) dalam Melarutkan Kalsium’, Jurnal Akademika Kimia, 3(4), pp. 214–221.
Wink, M. (2008) ‘Plant secondary metabolism: diversity, function and its evolution’, Natural Product Communications, 3(8), p. 1934578X0800300801.
Zirconia, A., Kurniasih, N. and Amalia, V. (2015) ‘Identifikasi Senyawa Flavonoid Dari Daun Kembang Bulan (Tithonia Diversifolia) Dengan Metode Pereaksi Geser’, al-Kimiya, 2(1), pp. 9–17. doi: 10.15575/ak.v2i1.346.
DOI: https://doi.org/10.22146/mot.72163
Article Metrics
Abstract views : 2237
| 
views : 864
Refbacks
- There are currently no refbacks.
Copyright (c) 2023 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada