Hypolipidemic Potential of Ethyl acetate Extract of Hyphaene thebaica Fruit in Streptozotocin-induced Diabetic Rats

https://doi.org/10.22146/mot.75401

Mubarak Muhammad Dahiru(1*), Nadro Margret Samuel(2)

(1) - Department of Biochemistry, Modibbo Adama University, Yola - Department of Science Laboratory Technology, Adamawa State Polytechnic Yola
(2) Department of Biochemistry, Modibbo Adama University, Yola
(*) Corresponding Author

Abstract


Hyperlipidemia is characterized by elevated levels of Triglycerides, Cholesterol, LDL, and decreased levels of HDL are challenges in the management of Diabetes mellitus which might lead to death. This study aimed to determine the hypolipidemic potential of ethyl acetate extract of Hyphaene thebaica fruit in streptozotocin-induced diabetic rats. The hypolipidemic potential of H. thebaica fruit in streptozotocin-induced diabetic rats was determined. Rats were divided into six groups. Treatment groups were administered the extract at doses of 400 and 200 mg/kg body weight. Oral administration of the extract at 400 mg/kg body weight for four weeks significantly (p < 0.05) decreased the levels of total cholesterol (151.7 mg/dl ±2.40), Triglyceride (84.0 mg/dl ±1.15), LDL (75.2 mg/dl ±1.20) and increased HDL (39.3 mg/dl ±1.88) level. However no significant (p < 0.05) difference was observed between the 400 and 200 mg/kg body weight dose. Conclusively, H. thebaica can be used as a medicinal plant due to its protective action against dyslipidemic complications of diabetes mellitus.

Keywords


Hypolipidemic; Doum Palm; Dyslipidemia; Diabetes

Full Text:

PDF


References

Abdulazeez, M. A., Bashir, A., Adoyi, B. S., Mustapha, A. Z., Kurfi, B., Usman, A.Y. and Bala, R. K. (2019). Antioxidant, Hypolipidemic and Angiotensin-Converting Enzyme Inhibitory Effects of Flavonoid-Rich Fraction of Hyphaene thebaica (Doum Palm) Fruits on Fat-Fed Obese Wistar rats. Asian J. Res. Biochem. 5, 1-11.

Aboshora, W., Lianfu, Z., Dahir, M., Qingran, M., Qingrui, S., Jing, L., Al-Haj, N. Q. M. and Ammar A. (2014). Effect of extraction method and solvent power on polyphenol and flavonoid levels in Hyphaene Thebaica L Mart (Arecaceae) (Doum) fruit, and its antioxidant and antibacterial activities. Trop. J. Pharm. Res. 13(12), 2057–2063.

ADA (American Diabetes Association). (2021a). Classification and Diagnosis of Diabetes. Diab. Car. 44(1), S15 – S33.

Al-Hariri, M. T. (2012). Comparison of the rate of diabetes mellitus induction using streptozotocin dissolved in different solvents in male rats. J. Comp. Clin. Path. Res. 1(3), 96–99.

Bayad, A. E. (2016). Influences of doum fruit (Hyphaene thebaica) extract on the reproductive parameters, blood picture, lipid profile, and hepato-renal functions in rats. Merit Res. J. Med. Med. Sci. 4, 384-391.

Chandra, S. and Goyal, A. T. (2014). Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. Am. J. Ethnomed. 1(4), 244-249.

Daniel, G. and Krishnakumari, S. (2015). Quantitative analysis of primary and secondary metabolites in aqueous hot extract of Eugenia uniflora (L) leaves. Asian J. Pharm. Clin. Res. 8(1), 334-338.

Decroli, E. (2019). Diabetes Mellitus type 2. Central Publishing of Internal Medicine Department, Faculty of Medicine, Andalas University (2019): 1-2.

Denise, R. F. (2017). Lippincott’s Illustrated Reviews: Biochemistry Fifth Edition. Lippincott Williams and Wilkins, a Wolters Kluwer Health, Hong Kong.

Edgar Romualdo, E. G., Lilia, A. M., Rafael, S. G., & Alfredo, S. M. (2018). Antioxidant effects of damiana (Turnera diffusa Willd. ex Schult.) in kidney mitochondria from streptozotocin-diabetic rats. Nat. Prod. Res. 32(23), 2840–2843. https://doi.org/10.1080/14786419.2017.1380009.

El Halim H. N. (2020). Effect of Doum Fruit (Hyphaene Thebaica) Extract on Some Biochemical Parameters, Enzyme Activities and Histopathological Changes of Pancreas in Alloxan Induced Diabetic rats. Food Nut. Sci. 11, 207-219.

Elmarakby, A. A. and Sullivan, J. C. (2012). Relationship between oxidative stress and inflammatory cytokines in diabetic nephropathy. Cardiovasc. Ther. 30(1), 49–59.

Friedewald, W. T., Levy, R. I., & Fredrickson, D. S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18(6), 499–502.

Ghada, A., Taha, I. B., Abdel-Farid, H. A., Elgebaly, U. A., Mahalel, M. G., Sheded, M. B. et al. (2020). Metabolomic Profiling and Antioxidant, Anticancer and Antimicrobial Activities of Hyphaene thebaica. Processes, 8(3), 266.

Han, D. G., Cho, S. S., Kwak, J. H. and Yoon, I. (2019).Medicinal plants and phytochemicals for diabetes mellitus: pharmacokinetic characteristics and herb-drug interactions. J. Pharm. Invest. 49, 603–612.

Huyut, Z., Beydemir, S. and Gülçin, I. (2017) Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochem. Res. Int. 2017, Article ID: 7616791.

IDF (International Diabetes Federation). (2019). Worldwide toll of diabetes. IDF Diabetes Atlas ninth Edition. Inis communications, Chiang Mai, Thailand.

Jayaraman, R., Subramani, S., Sheik Abdullah, S. H., & Udaiyar, M. (2018). Antihyperglycemic effect of hesperetin, a citrus flavonoid, extenuates hyperglycemia and exploring the potential role in antioxidant and antihyperlipidemic in streptozotocin-induced diabetic rats. Biomed. Pharmacother. 97, 98–106. https://doi.org/10.1016/j.biopha.2017.10.102

Lamiaa, A. G. and Laith, Z. F. (2018). Antioxidant activity of two different extracts from Doum (Hyphaene thebaica) fruits. J. Pharm. Bio. Sci. 13(4):30-33.

Lotfy, M., Adeghate, J., Kalasz, H., Singh, J., Adeghate, E. (2017). A Chronic Complications of Diabetes Mellitus: A Mini Review. Curr. Diab. Rev. 13: 3-10.

Mohd, I. Y., Archana, S., Arumugam, G., Mahmoud, A. and Kuldeep, D. (2017). Promising Antidiabetic Drugs, Medicinal Plants and Herbs: An Update. Int. J. Pharm. 13(7), 732-745.

NNREC (Norwegian National Research Ethics Committee). (2019). Ethical guidelines for the use of Animals in Research. Oslo.

Ozder, A. (2014). Lipid profile abnormalities seen in T2DM patients in primary healthcare in Turkey: a cross-sectional study. Lipids in Health and Dis. 13: 183.

Patel, S., Santani, D., Shah, M., & Patel, V. (2012). Anti-hyperglycemic and Anti-hyperlipidemic Effects of Bryonia Laciniosa Seed Extract and its Saponin Fraction in Streptozotocin-induced Diabetes in Rats. J. Young. Pharm. 4(3), 171–176. https://doi.org/10.4103/0975-1483.100024

Sani, I., Abdulhamid, A., Bello, F., Fakai, I. M. and Nassir, S. A. (2015). Phytochemical Compositions and In Vitro Antioxidant Potentials of Some Wild Edible Fruits Found in Northern Part of Nigeria. Academica Editores: Acta Velit 1(4), 100-107.

Schofield, J. D., Liu, Y., Rao-Balakrishna, P., Malik, R. A., & Soran, H. (2016). Diabetes Dyslipidemia. Diab. Ther. 7(2), 203–219. https://doi.org/10.1007/s13300-016-0167-x.

Stein, E. A. (1987). Lipids, lipoproteins and Apolipoproteins. In: Teitz, N. W. (Ed). Fundamentals of Clinical Chemistry, 3rd Edn. W.B Sauders Philadelphia.

Tezcan, F., Kolayli, S., Ulusoy, H. S. E. & Erim, F. B. (2011). Evaluation of organic acid, saccharide composition and antioxidant properties of some authentic Turkish honeys. J. Food Nut. Res. 50, 33-40. ISSN: 1336-8672.

Tietz, N. W. (1990). Clinical Guide to Laboratory Test, Second Edition W.B. Saunders Company, Philadelphia, U.S.A. 554-556.

Tohamy, A. A., Mohga, S. A., Adel, K. I., Khaled, F. M. and Rasha S. M. (2013). The Effect of Lupinus Albus (Termis) and Hyphaene Thebaica (Doum) on Some Biochemical Parameters in Streptozotocin-Induced Diabetic rats. Egypt. J. Hosp. Med. 53, 789–794.

Trease, G. E. and Evans W. C. (2009). Text book of Pharmacognosy. 16th ed. Saunders Ltd, London.

Victor, W. R, Bender, D. A., Bothman, K. M., Kennelly, P. J. & Anthony, W. P. (2018). Harper’s Illustrated Biochemistry Thirty-First edition. McGraw-Hill Education/Medical Publishing Division, New York. ISBN-10 1259837939.

Wacnic, R. G. and Alber, J. J. (1978). A comprehensive evaluation of the heparin manganese precipitation procedure for estimating high density lipoprotein cholesterol. J. Lipid Res. 19, 65-76.

WHO (World Health Organization). (2019). Classification of diabetes mellitus. World Health Organization, Department for Management of Non-communicable Diseases, Disability, Violence and Injury Prevention. Switzerland. ISBN 978-92-4-151570-2.



DOI: https://doi.org/10.22146/mot.75401

Article Metrics

Abstract views : 1567 | views : 1330

Refbacks



Bookmark and Share


Copyright (c) 2022 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada