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* Corresponding Author
The in-vitro Antioxidant Properties of Crude Drugs Traditionally Used for Diabetes Management in Northern Banyumas
https://doi.org/10.22146/mot.76958 Dwi Hartanti(1), Shintia Lintang Charisma(2), Widya Agustina(3), Rizky Destya Sary(4), Denia Awanda Putri(5), Alwani Hamad(6*)
(1) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Central Java
(2) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Central Java
(3) Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Central Java
(4) Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Central Java
(5) Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Central Java
(6) Department of Chemical Engineering, Faculty of Engineering and Science, Universitas Muhammadiyah Purwokerto, Purwokerto, Central Java
(*) Corresponding Author
Abstract
Chinaberry (Melia azedarach L.) leaves, Malayan cherry (Muntingia calabura L.) fruits, and Yacon (Smallanthus sonchifolius (Poepp.) H.Rob.) leaves are used for traditional diabetes treatment by Banyumas (Central Java, Indonesia) people. This study characterized selected quality parameters, evaluated the in-vitro antioxidant activity as the preliminary assay for its antidiabetic activity, and calculated the total flavonoid content (TFC) and total phenolic content (TPC) of those crude drugs. The plant materials of each species were collected from three different areas in Banyumas and dried into crude drugs. The quality parameters were determined according to the standard method in the Indonesian Herbal Pharmacopeia (IHP) 2017. The antioxidant activity was evaluated by the standard 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric reducing antioxidant power (FRAP) assays, while TFC and TPC were analyzed following standard methods in IHP 2017. The correlations between antioxidant activities and the TFC-TPC were analyzed by Pearson's correlation test. The values for acid-insoluble ash, loss on drying, total ash, ethanol extractable, and water-extractable of all crude drugs were quantified, with TPC and TFC proposed as the chemical content parameter for Malayan cherry fruits and Yacon leaves. Yacon leaves and Malayan cherry fruits contained the highest TFC and TPC at 8.01±0.72 mg Quercetin equivalent (QE)/g dry weight (DW) and 11.54±1.44 mg Gallic acid equivalent (GAE)/g DW, respectively. Malayan cherry fruits exerted the strongest DPPH scavenging activity (366.13±17.65 mM Trolox equivalent (TE)/g DW) and FRAP (1025.33±50.47 mM TE/g DW). Moderate to strong correlations were observed between DPPH radical scavenging activity – FRAP and TFC-TPC of Malayan cherry fruits and Yacon leaves. Hence, flavonoids and phenolic compounds of both crude drugs contributed to their antioxidant activity.
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Agarwal, M., Rai, V., Khatoon, S. & Mehrotra, S., 2014. 'Effect of microbial load on therapeutically active constituent glycyrrhizin of Glycyrrhiza glabra L'. Indian J. Tradit. Knowl. 13, 319–324.
Alamgir, A.N.M. 2017. 'Herbal Drugs: Their Collection, Preservation, and Preparation; Evaluation, Quality Control, and Standardization of Herbal Drugs', in: Rainsford, K.D. (Ed.), Therapeutic Use of Medicinal Plants and Their Extracts: Volume 1. Springer International Publishing, Cham, pp. 453–495.
Aligita, W., Susilawati, E., Sukmawati, I.K., Holidayanti, L. & Riswanti, J., 2018. 'Antidiabetic activities of Muntingia calabura L. leaves water extract in type 2 diabetes mellitus animal models'. Indones. Biomed. J. 10, 165–70.
Bandeira, S. de M., Da-Fonseca, L.J.S., Guedes, G. da S., Rabelo, L.A., Goulart, M.O.F. & Vasconcelos, S.M.L., 2013. 'Oxidative stress as an underlying contributor in the development of chronic complications in diabetes mellitus'. Int. J. Mol. Sci. 14, 3265–3284.
de Almeida Paula, H.A., Abranche, M.V. & de Luces Fortes Ferreira, C.L., 2015. 'Yacon (Smallanthus sonchifolius): A food with multiple functions'. Crit. Rev. Food Sci. Nutr. 55, 32–40.
Dougnon, G. & Ito, M., 2022. 'Essential oils from Melia azedarach L. (Meliaceae) leaves: Chemical variability upon environmental factors'. J. Nat. Med. 76, 331–341.
Ervina, M., Poerwono, H., Widyowati, R., Matsunami, K. & Sukardiman, 2020. 'Bio-selective hormonal breast cancer cytotoxic and antioxidant potencies of Melia azedarach L. wild type leaves'. Biotechnol. Reports 25, e00437.
Hong, S.S., Lee, S.A., Han, X.H., Lee, M.H., Hwang, J.S., Park, J.S., Oh, K.W., Han, K., Lee, M.K., Lee, H., Kim, W., Lee, D. & Hwang, B.Y., 2008. 'Melampolides from the leaves of Smallanthus sonchifolius and their inhibitory activity of LPS-induced nitric oxide production'. Chem. Pharm. Bull. 56, 199–202.
Indonesian MoH, 2017. Indonesian Herbal Pharmacopeia 2017, 2nd ed. Ministry of Health Republic of Indonesia, Jakarta.
Khajehei, F., Niakousari, M., Damyeh, M.S., Merkt, N., Claupein, W. & Graeff-Hoenninger, S., 2017. 'Impact of ohmic-assisted decoction on bioactive components extracted from yacon (Smallanthus sonchifolius Poepp.) leaves: Comparison with conventional decoction'. Molecules 22, 2043.
Kharel, R. & Sharma, K.R., 2020. 'Evaluation of antioxidant potential and quantitative estimation of phenolic and flavonoid content in some selected Nepalese medicinal plants'. Asian J. Pharm. Clin. Res. 13, 124–128.
Kolar, F.R., Kamble, V.S. & Dixit, G.B., 2011. 'Phytochemical constituents and antioxidant potential of some underused fruits'. African J. Pharm. Pharmacol. 5, 2067–2072.
Kumazawa, S., Kubota, S., Yamamoto, H., Okamura, N., Sugiyamab, Y., Kobayashia, H., Nakanishi, M., Ohta, T., Sugiyama, Y., Kobayashi, H., Nakanishi, M. & Ohta, T., 2013. 'Antiangiogenic activity of flavonoids from Melia azedarach'. Nat. Prod. Commun. 8, 1719–1720.
Lin, J.T., Chen, Y.C., Chang, Y.Z., Chen, T.Y. & Yang, D.J., 2017. 'Effective compounds in the fruit of Muntingia calabura Linn. cultivated in Taiwan evaluated with scavenging free radicals and suppressing LDL oxidation'. Food Funct. 8, 1504–1511.
Mahmood, N.D., Nasir, N.L.M., Rofiee, M.S., Tohid, S.F.M., Ching, S.M., Teh, L.K., Salleh, M.Z. & Zakaria, Z.A., 2014. 'Muntingia calabura: A review of its traditional uses, chemical properties, and pharmacological observations'. Pharm. Biol. 52, 1598–1623.
Mwamatope, B., Tembo, D., Chikowe, I., Kampira, E. & Nyirenda, C., 2020. 'Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants'. Sci. African 9, e00481.
Nasution, F., Theanhom, A.A., Unpaprom, Y., Ramaraj, R., Manmai, N. & Chumpookam, J., 2022. 'Muntingia calabura fruits as sources of bioactive compounds and fermentative ethanol production'. Biomass Convers. Biorefinery.
Padilla-González, G.F., Amrehn, E., Frey, M., Gómez-Zeledón, J., Kaa, A., Da-Costa, F.B. & Spring, O., 2020. 'Metabolomic and gene expression studies reveal the diversity, distribution and spatial regulation of the specialized metabolism of yacón (Smallanthus sonchifolius, Asteraceae)'. Int. J. Mol. Sci. 21, 4555.
Park, S.J., Nhiem, N.X., Subedi, L., Oh, I., Kim, J.-Y., Kim, S.Y. & Kim, S.H., 2020. 'Isolation of bioactive limonoids from the fruits of Melia azedarach'. J. Asian Nat. Prod. Res. 22, 830–838.
Pereira, G.A., Arruda, H.S., Morais, D.R. de, Eberlin, M.N. & Pastore, G.M., 2018. 'Carbohydrates, volatile and phenolic compounds composition, and antioxidant activity of calabura (Muntingia calabura L.) fruit'. Food Res. Int. 108, 264–273.
Peter, A.E., Satyavani, T.R., Amenbaby, G., Ulabala, S.V.K., Sudhakar, P., Rajeswari, M., Sandeep, B.V., Rao, B.G. & Kalpana, V.L., 2020. 'Preliminary phytopharmacological analysis, proximate composition analysis and product development with the leaves and fruits of Muntingia calabura L'. Res. J. Pharm. Technol. 13, 4603–4612.
Pisoschi, A.M., Pop, A., Cimpeanu, C. & Predoi, G., 2016. 'Antioxidant capacity determination in plants and plant-derived products: A review'. Oxid. Med. Cell. Longev. 2016, 9130976.
Pubchem.2022. Structur of caffeic acid, myricetin, quercetin. Available online at https://pubchem.ncbi.nlm.nih.gov/. Accessed on October 2022.
Qiu, L., Heng, L., Xu, R., Luo, J. & Li, Y., 2019. 'Two new nimbolinin- and trichilin-class limonoids isolated from the fruits of Melia azedarach'. Chin. J. Nat. Med. 17, 227–230.
Ramonah, D., Rahardhian, M.R.R. & Putri, C.N., 2020. 'Determinasi total flavonoid, total fenolik, dan aktivitas antibakteri ekstrak etanol daun insulin (Smallanthus sonchifolius) dengan metode perkolasi'. Media Farm. Indones. 15, 11585–1592.
Rotta, E.M., Haminiuk, C.W.I., Maldaner, L. & Visentainer, J.V., 2017. 'Determination of antioxidant activity and phenolic compounds of Muntingia calabura Linn. peel by HPLC-DAD and UPLC-ESI-MS/MS'. Int. J. Food Sci. Technol. 52, 954–963.
Russo, D., Malafronte, N., Frescura, D., Imbrenda, G., Faraone, I., Milella, L., Fernandez, E. & de-Tommas, N., 2015. 'Antioxidant activities and quali-quantitative analysis of different Smallanthus sonchifolius [(Poepp. and Endl.) H. Robinson] landrace extracts'. Nat. Prod. Res. 29, 1673–1677.
Russo, Daniela, Valentão, P., Andrade, P.B., Fernandez, E.C. & Milella, L., 2015. 'Evaluation of antioxidant, antidiabetic and anticholinesterase activities of Smallanthus sonchifolius landraces and correlation with their phytochemical profiles'. Int. J. Mol. Sci. 16, 17696–17718.
Santos-Sánchez, N.F., Salas-Coronado, R., Villanueva-Cañongo, C. & Hernández-Carlos, B., 2019. 'Antioxidant compounds and their antioxidant mechanism', in: Shalaby, E. (Ed.), Antioxidant. IntechOpen, pp. 1–28.
Santos, K.C. Dos, Bueno, B.G., Pereira, L.F., Francisqueti, F.V., Braz, M.G., Bincoleto, L.F., Da-Silva, L.X., Ferreira, A.L.A., Nakamune, A.C. de M.S., Chen, C.Y.O., Blumberg, J.B. & Corrêa, C.R., 2017. 'Yacon (Smallanthus sonchifolius) leaf extract attenuates hyperglycemia and skeletal muscle oxidative stress and inflammation in diabetic rats'. Evidence-Based Complement. Altern. Med. 2017, 6418048.
Seifu, D., Gustafsson, L.E., Chawla, R., Genet, S., Debella, A., Holst, M. & Hellström, P.M., 2017. 'Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus'. J. Exp. Pharmacol. 9, 23–29.
Shrestha, S.S., Ferrarese, I., Sut, S., Zengin, G., Grana, S., Ak, G., Pant, D.R., Dall’Acqua, S. & Rajbhandary, S., 2021. 'Phytochemical investigations and in vitro bioactivity screening on Melia azedarach L. leaves extract from Nepal'. Chem. Biodivers. 18, e2001070.
Simamora, A., Santoso, A.W., Rahayu, I. & Timotius, K.H., 2020. 'Enzyme inhibitory, antioxidant, and antibacterial activities of ethanol fruit extract of Muntingia calabura Linn'. J. Herbmed Pharmacol. 9, 346–354.
Sugahara, S., Ueda, Y., Fukuhara, K., Kamamuta, Y., Matsuda, Y., Murata, T., Kuroda, Y., Kabata, K., Ono, M., Igoshi, K., Yasuda, S., Kamamut, Y., Matsuda, Y., Murat, T., Kuroda, Y., Kabata, K., Ono, M., Igoshi, K. & Yasuda, S., 2015. 'Antioxidant effects of herbal tea leaves from yacon (Smallanthus sonchifolius) on multiple free radical and reducing power assays, especially on different superoxide anion radical generation systems'. J. Food Sci. 80, C2420–C2429.
Sultana, S., Asif, H.M., Akhtar, N., Waqas, M. & Ur-Rehman, S., 2014. 'Comprehensive review on ethnobotanical uses, phytochemistry and pharmacological properties of Melia azedarach Linn'. Asian J. Pharm. Res. Heal. Care 6, 26–32.
Tauheed, A., Hamiduddin, Khanam, S., Ali, M.A. & Zaigham, M., 2017. 'Comparative physicochemical evaluation of kharekhasak (Tribulus terrestris Linn.) before and after mudabbar process'. Pharmacognosy Res. 9, 384–389.
Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L. & Byrne, D.H., 2006. 'Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts'. J. Food Compos. Anal. 19, 669–675.
Utaminingrum, W., Nofrianti & Hartanti, D., 2020. 'Ethnomedicinal survey of traditional antidiabetic plants in Baturraden and Sumbang'. Medisains J. Ilm. Ilmu-ilmu Kesehat. 18.
Yunus, S.N.M., Abas, F., Jaafar, A.H., Azizan, A., Zolkeflee, N.K.Z. & Ghafar, S.Z.A., 2021. 'Antioxidant and α-glucosidase inhibitory activities of eight neglected fruit extracts and UHPLC-MS/MS profile of the active extracts'. Food Sci. Biotechnol. 30, 195–208.
Zeng, J., Ma, R.-J., Wang, L., Zhang, S.-N., Song, H.-Z., Yang, Y. & Tan, Q.-G., 2019. 'Chemical constituents from the leaves of Melia azedarach'. Nat. Prod. Res. 33, 2860–2863.
Zolkeflee, N.K.Z., Ismail, N.A., Maulidiani, M., Hamid, N.A.A., Ramli, N.S., Azlan, A. & Abas, F., 2021.' Metabolite variations and antioxidant activity of Muntingia calabura leaves in response to different drying methods and ethanol ratios elucidated by NMR-based metabolomics'. Phytochem. Anal. 32, 69–83. DOI: https://doi.org/10.22146/mot.76958
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