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* Corresponding Author
Isolation and Identification of β-sitosterol, 7-hydroxystigmast-22-en-3,6-dione and 3β, 24(S)-dihydroxycholesta-5, 25-diene-7-one from stem bark of Nauclea pobeguinii
https://doi.org/10.22146/mot.77324 Adewusi John Adepoju(1), Sunday Olusegun Oladoye(2*), Ezekiel Temidayo Ayodele(3), Adeola Victoria Falade(4), Gin Keat Lim(5), Chuan Wei OO(6)
(1) Organic Chemistry Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State
(2) Organic Chemistry Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State
(3) Organic Chemistry Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State
(4) Organic Chemistry Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State
(5) School of Chemical Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang
(6) School of Chemical Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang
(*) Corresponding Author
Abstract
The stem bark of the Nauclea pobeguinii was collected, air-dried, and pulverized and was extracted with solvent of varying polarity (n-hexane, ethyl acetate, and ethanol) to obtain the crude extracts. Silica gel column and thin layer chromatographic separation afforded three compounds whose structures were elucidated as β-sitosterol (1), 7-hydroxystigmast-22-en-3.6-dione (2), and 3β, 24(S)-dihydroxycholesta-5, 25-dien-7-one (3) by analysis of their chemical and spectral characteristic from 1D and 2DNMR, FTIR and by comparing of data with those reported in the literature.
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DOI: https://doi.org/10.22146/mot.77324
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Universitas Gadjah Mada