Skin Histopathology of Diabetes Mellitus Rats Treated with Edible Nest Swiftlets (Aerodhramus fuciphagus) Ointment

https://doi.org/10.22146/mot.79392

Dita Ayulia Dwi Sandi(1*), Yaumi Musfirah(2)

(1) Lambung Mangkurat University, Banjarbaru, South Borneo
(2) Borneo Lestari University, Banjarbaru, South Borneo
(*) Corresponding Author

Abstract


The edible nest swiftlets are suspected to contain sialic acid that can increase the process of cell growth that can reduce the formation of scar tissue and accelerate wound healing. This study aimed to determine the effect and the optimal dosage of edible nest swiftlets ointment on skin histopathology of diabetes mellitus rats that suffered a scratch wound. This study used 25 male white rats, divided into 5 groups, all groups were treated with betadine and the intervention of group 1 was vaseline only (control group), group 2, 3, and 4 were edible nest swiftlets (ENS) ointment with 10%, 20%, and 30% concentration and group 5 were the sanoskin as control positive. The rats were injured in the back area and given the treatment according to the group once a day for 14 days. Skin tissue was taken to make histopathologic preparations for observation on the 0th, 7th, and 14th days. The parameters observed included the number of macrophages, neo-capillarization, and fibroblasts. The result on the 14th day showed that the number of macrophages in the control group, sanoskin group, and ENS 10%, 20%, and 30% group was 3.8±5.019; 3.2±2.489; 1.84±2.387; 1.8±2.049 and 1.2±1.095. The result of neocapillilization were 1.28±1.673; 1.4±1.673; 2.8±1.778; 5.4±4.159; and 15±30.773. The conclusion showed there was a significant difference and there was the effect of edible nest swiftlets ointment on the number of macrophages, the number of neocapillilization, and the density of fibroblasts on the rats with diabetes mellitus that suffered a scratch wound.

Keywords


Edible nest swiftlet; Aerodhramus fuciphagus; skin histopathology; Ointment; wound healing

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References

Butler, L.K. 1995. Regulation of Blood Glucose Levels in Normal and Diabetic Rats. Texas: Division of Biological Sciences. 181-202.

Depkes RI. 2005. Pharmaceutical Care untuk penyakit Diabetes Mellitus. Departemen Kesehatan Republik Indonesia. Jakarta.

Fitriani, N. 2016. Uji Aktivitas Gel Etil P- Metoksinamat terhadap Penyembuhan Luka Terbuka pada Tikus Putih (Rattus novegicus) Jantan. Skripsi. UIN Syarif Hidayatullah, Jakarta.

Gope, M.L. & Gope R. 2007. Topically applied EGF and PDGFs affect positively the co-ordinate expression of EGF and PDGF receptor genes during the acute cutaneous wound-healing process. Current Sciences. Vol. 92, No. 5.

Irma. 2014. Pemberian krim ekstrak sarang burung walet 10% meningkatkan epitelisasi pada penyembuhan luka kulit mencit (Mus musculus). Tesis. Universitas Udayana Denpasar.

Kyung, B.R., et al. 2012. Mechanisms of Edible nest swiftlets Extract Induced Proliferation of Human Adipose-Derived Stem Cells. Evidence-Based Complementary and Alternative Medicine. Vol. 2012.

Konsul Perdangan. 2016. Laporan Market Brief : edisi Januari 2016 Sarang Burung Walet di Pasar Hongkong. Konsulat Jenderan Republik Indonesia Hongkong SAR. Republik Rakyat Tiongkok.

Nakagawa, H., Hama, Y., Sumi, T., Li, S., Maskos, K., Kalayanamitra, K., Mizumoto, S., Sugahara, K. & Teh, L. 2007. Occurrence of a nonsulfated chondroitin proteoglycan in the dried saliva of Collocalia swiftlets (edible bird’s-nest). Glycobiology. 17 (2): 157–164.

Napanggala, Susianti, Apriliana, A. 2014. Pengaruh pemberian getah tanaman jarak pagar (Jatropa curcas L.) secara topikal terhadap tingkat kesembuhan luka iris pada tikus putih jantan galur Sparague dawley. ISSN 2337-3776.

Novriansyah, R. 2008. Perbedaan kepadatan kolagen di sekitar luka insisi tikus wistar yang dibalut kasa konvensional dan penutup oklusif hidrokoloid selama 2 dan 14 hari. Semarang : Universita Diponegoro.

Prasetyo, B.F., Ietje, W., Bambang, P.P. 2010. Aktivitas Sediaan Gel Ekstrak Batang Pohon Pisang ambon dalam Proses penyembuhan luka pada mencit. Jurnal Veteriner. ISSN : 1411-8327. Vo. 11 No. 2:70-73.

Rolf, K.J. & Grobbelaar, A.O. 2012. A Review of fetal scarless healing. ISRN Dermatology. 2012: 1-9.

Sandi, D.A.D and Musfirah, Y. 2018. Pengaruh Basis Salep Hidrokarbon dan Basis Salep Serap terhadap Formulasi Salep Sarang Burung Walet Putih (Aerodramus fuciphagus). Jurnal Ilmiah Manuntung. 4 (2) : 149-155.

Sandi, D.A.D and Musfirah, Y. 2019. Wound-healing Effects of Edible nest swiftlets Oinment (Aerodramus fuciphagus) in Alloxan-Induced Male Rats. Traditional Medicine Journal. 24 (1): 33-39.

Tung, C.H., Pan, J.Q., Chang, H.M. & Chou, S.H. 2008. Authentic determination of Birds’ nests by saccharides profile. Journal of Food and Drug Analysis. 16 (4): 86-91.



DOI: https://doi.org/10.22146/mot.79392

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