I Made Sukadana(1*), Sri Rahayu Santi(2)

(1) Department of Chemistry, Faculty of Mathematics and Science, University of Udayana
(2) Department of Chemistry, Faculty of Mathematics and Science, University of Udayana
(*) Corresponding Author


Isolation of antibacterial compounds from n-hexane extract of tempuyung leaf (Sonchus Arvensis L.) has been carried out. n-hexane extract (9,15 g) showed  antibacterial inhibition zone of 9 mm on Staphylococcus aureus and 8 mm on Escherichia coli at concentration of 1000 ppm.  Fractionation of n-hexane extract using column chromatography resulted on 5 fractions (F1, F2, F3, F4, and F5). Antibacterial test of the fractions toward Staphylococcus aureus and Escherichia coli showed that fraction F2 and F4 positive toward Staphylococcus aureus and Escherichia coli. Fraction F3 showed just positive toward Escherichia coli while fraction F5 just positive toward Staphylococcus aureus. Further separation using preparative thin layer chromatography (TLC) technical resulted in 3 fractions (F4.1, F4.2 and F4.3 fractions), in which F4.1 fraction indicates the most active inhibition growth test again bacterial with diametric inhibition zone of 9 mm towards Staphylococcus aureus and 11 mm for Escherichia coli within concentration of 100 ppm.   Gas Chromatography–Mass Spectroscopy (GC-MS) was employed in order to identity the F4.1 fraction. From WILEY7 database, it was identified  that isolate are mixture  of 2  compound components namely bis (2-ethylhexyl) ester and possibility of new compound included triterpenoid group  with molecule formula C32H66O6.


Sonchus Arvensis L., antibacterial, isolation, identification


Chairul, 1999, Tempuyung Untuk Menghadang Asam Urat, Intisari Tanaman Obat, 22 September 2007

Cos, P., Ying, L., Calomme, M., Jia P. Hu, Cimanga, K., Poel, B. V., Pieters, L., Arnold J. Vlietinck, and Berghe, D. V., 1998, Structure-Activity Relationship and Classification of Flavonoids as Inhibitors of Xanthin Oxidase and Superoxide Scavengers, J.Nat.Prod. 61; 71-76

Elsya, 2004, Patroli Asam Urat dengan Tanaman Berkhasiat, Pikiran Rakyat 25 Oktober 2007

Kapiten, 2003, Tanaman Untuk Menurunkan Kolesterol Tinggi, www.forumWebgaul .com /archive/thread/t-50955-tanaman-untuk-menurunkan-kolesterol-tinggi.htmk

Silverstein, Bassler and Morrill, 1981, Identification of Organic Coumpound, Fourth edition, Singapore 25 Oktober 2007


Article Metrics

Abstract views : 1781 | views : 4011


  • There are currently no refbacks.

Copyright (c) 2011 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada