ANTIBACTERIAL COMPOUNDS PRESENT ON n-HEXANE EXTRACT OF TEMPUYUNG LEAVES ( Sonchus arvensis L.)

Abstract

Isolation of antibacterial compounds from n-hexane extract of tempuyung leaf (Sonchus Arvensis L.) has been carried out. n-hexane extract (9,15 g) showed  antibacterial inhibition zone of 9 mm on Staphylococcus aureus and 8 mm on Escherichia coli at concentration of 1000 ppm.  Fractionation of n-hexane extract using column chromatography resulted on 5 fractions (F₁, F₂, F₃, F₄, and F₅). Antibacterial test of the fractions toward Staphylococcus aureus and Escherichia coli showed that fraction F₂ and F₄ positive toward Staphylococcus aureus and Escherichia coli. Fraction F₃ showed just positive toward Escherichia coli while fraction F₅ just positive toward Staphylococcus aureus. Further separation using preparative thin layer chromatography (TLC) technical resulted in 3 fractions (F_4.1, F_4.2 and F_4.3 fractions), in which F_4.1 fraction indicates the most active inhibition growth test again bacterial with diametric inhibition zone of 9 mm towards Staphylococcus aureus and 11 mm for Escherichia coli within concentration of 100 ppm.   Gas Chromatography–Mass Spectroscopy (GC-MS) was employed in order to identity the F_4.1 fraction. From WILEY7 database, it was identified  that isolate are mixture  of 2  compound components namely bis (2-ethylhexyl) ester and possibility of new compound included triterpenoid group  with molecule formula C₃₂H₆₆O₆.