Nuraeni Ekowati(1*), Rina Sri Kasiamdari(2), Nursamsi Pusposendjojo(3), C.J. Soegihardjo(4)

(1) Faculty of Biology, University of Jenderal Soedirman Faculty of Biology, Universitas Gadjah Mada
(2) Faculty of Biology, Universitas Gadjah Mada
(3) Faculty of Agriculture, Universitas Gadjah Mada
(4) Faculty of Pharmacy, University of Sanata Dharma
(*) Corresponding Author


Shiitake mushroom (Lentinula edodes (Berk.) Pegler) is one of the fungi that has potentially been used as food and medicinal mushroom. Cultivation in liquid medium by the fermentation process has been developed over recent years. Four isolates of L.edodes (isolates from Malang, Cianjur, Lembang and Yogyakarta) cultured on three types of fermentation media (KM: Medium Kauffman; GYMT: Glucose, Yeast Extract, Malt Extract, Thiamin; YEMR: Yeast extract, Malt Extract, Rice bran). Bioactive metabolites  extracted from mycelium biomass and fermentation culture filtrates were tested using pathogenic microbial Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Candida albicans ATCC 10231, and Trichophyton mentagrophytes. Inhibition zone diameter data were analyzed using analysis of variance followed by Duncan test at 5% error rate. The results showed that bioactive compounds in the culture filtrates and mycelium biomass of L. edodes extracted using chloroform, ethyl acetate and water, were able to inhibit S. aureus, E. coli and C. albicans,  but were not able to inhibit T.mentagrophytes. Isolates of L.edodes from Lembang and Yogyakarta cultured on Kauffman Medium gave  the best results with inhibitory zones of 24.97 to 31.14 mm. Microbes which gave the most sensitive of bioactive compounds test from L. edodes were C. albicans followed by E. coli, S. aureus and T. mentagrophytes. Bioactive metabolites from L. edodes inhibited potentially pathogenic microbes  from the group of  bacteria and  yeasts but did not inhibit the fungus.


Lentinula edodes, fermentation medium, antimicrobial, bioactive metabolites


Ayodele, S.M. and M. E.Idoko. 2011. Antimicrobial activities of four wild edible mushrooms in Nigeria. I.J.S.N., 2(1) : 55-58

Fisher, F. and N.B. Cook. 1998. Fundamentals of Diagnostic Mycology. W.B. Saunders Company. London

Garraway, M.O. and R.C. Evans. 1984. Fungal Nutrition and Physiology. A John Wiley & Sons, Inc., Publication. New York

Griffin, D.H. 1994. Fungal Physiology. A John Wiley & Sons, Inc., Publication. New York

Hassegawa, R.H., M.C.M. Kasuya, M.C.D. Vanetti. 2005. Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes. Electron. J. Biotechno., 8 (2) : 212-217

Ishikawa, N.K., M.C.M. Kasuya, M.C.D. Vanetti. 2001. Antibacterial activity of Lentinula edodes growth in liquid medium. Braz. J. Microbiol., 32 (3) : 206-210

Jawetz, Melnick, dan Adelberg. 2008. Mikrobiologi Kedokteran. Ed. 23. Penerbit Buku Kedokteran, EGC, Jakarta

Lalitha, M.K. 2010. Manual on Antimicrobial Susceptibility Testing. Department of Microbiology, Christian Medical College Vellore, Tamil nadu

Manjunathan, J. and V. Kaviyarasan. 2011. Optimization of mycelia growth and antimicrobial activity of new edible mushroom, Lentinus tuberregium (Fr.). Tamil Nadu.,India. Int. J. Pharm. Tech. Res., 3(1): 497-504

Oyeka, C.A. 2000. Trichophyton mentagrophytes a keratinophilic fungus. Department of Microbiology, Nnamdi Azikiwe University Awka, Anambra State, Nigeria

Poucheret, P., F. Fons and S. Rapior. 2006. Biological and Pharmacological Activity of Higher Fungi: 20-Year Retrospective Analysis. Cryptogamie Mycol., 27 (4): 311-333

Quimio, T.H. 1978. Workbook in Tropical Mycology. Department of Plant Pathology, University of the Philippines at Los Banos College, Laguna Philippines

Rosa, L.H., K.M.G. Machado, C.C. Jacob, M. Capelari, C.A. Rosa and C.L. Zani. 2003. Screening of Brazilian Basidiomycetes for Antimicrobial Activity. Mem. Inst. Oswaldo Cruz., 98(7): 967-974

Smith, J.E., N.J. Rowan and R. Sullivan, 2002. Medicinal Mushrooms : Their therapeutic properties and current medical usage with special emphasis on cancer treatments. Cancer Research UK, University of Strathclyde

Sudarmono, P. 1992. Antibiotika dan masalah resistensi. Kursus Mikrobiologi, diselenggarakan atas kerjasama Dirjen Dikti-Unesco-Perhimpunan Mikrobiologi Cab. Jakarta, 1-3 Desember 1992, Universitas Indonesia, Jakarta

Vahidi, H., F. Kobarfard and F. Namjoyan. 2004. Effect of cultivation conditions on growth and antifungal activity of Mycena leptocephala. Afr. J. Biotechnol. 3 (11): 606-609

Yulinah, E. dan Mathilda. 1991. Mekanisme Kerja Antibiotika. Pusat Antar Universitas Bioteknologi, Institut Teknologi Bandung, Bandung


Article Metrics

Abstract views : 705 | views : 6900


  • There are currently no refbacks.

Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada