ANTIANGIOGENIC ACTIVITY OF METHANOLIC EXTRACT FROM AKAR PASAK BUMI (Eurycoma longifolia, Jack) ON CHORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYO INDUCED BY bFGF

https://doi.org/10.22146/tradmedj.8062

Nina Salamah(1*), Sugiyanto Sugiyanto(2), Mae Sri Hartati(3), Farida Hayati(4)

(1) Faculty of Pharmacy, University of Ahmad Dahlan
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Medicine, Universitas Gadjah Mada
(4) Department of Pharmacy, Islamic University of Indonesia
(*) Corresponding Author

Abstract


Angiogenesis is a neovascularization process which its function is to supply nutrient and oxygen for cell survival. Pasak bumi is one of the Indonesian original plants having anti cancer activity. Extract of methanol, buthanol, kloroform, and water akar pasak bumi was proved to have cytotoxic effect on cancer cell culture. This research aim to know activity of antiangiogenic extract of metanol akar pasak bumi at chorioallantoic membrane of chicken embryo. Antiangiogenic activity test conducted by divide 8-9 days old egg embrio into 8 groups. Group I as paper disc control, group II as bFGF control, group III as bFGF control + DMSO 0.8% solvent, group IV, V, VI, VII, and VIII as  groups of treatment. Each groups of treatment were gave by bFGF 1ng/µL extract of metanol akar pasak bumi respectively with concentration 20, 40, 60, 80 and 100 µg/mL concentration. After 3 days incubation, then the chorioallantoic membrane macroscopic and microcospically analized. The antiangiogenic activity extract of metanol akar pasak bumi at chorioallantoic membrane bFGF induce at used concentration, that is start from 40 µg/mL concentration. The increases of the concentration also increase the prohibition activity to angiogenesis.

 


Keywords


angiogenesis; Eurycoma longifolia; pasak bumi; CAM; bFGF



References

Anonim, 1989, Materia Medika Indonesia, Jilid V, Departemen Kesehatan Republik Indonesia, hal 212-215

Folkman, J,1996, Fighting Cancer by Attaching its Blood Supply, Scientific American 19: 116-119

Giavazzi, R., Albini, A., Bussolino, F., DeBraud, F., Presta, M., Ziche, M., Costa, A., 2000, The biological basis for antiangiogenic therapy (meeting report), European Journal of Cancer., 36: 1913-1918

Hanahan, D and Weinberg, R.A., 2000, The Hallmark of Cancer, Cell.,100:57-68

Jenie R.I., Meiyanto, E., Murwanti, R., 2006, Efek antiangiogenik ekstrak etanolik daun sambung nyawa (Gynura procumbens (Lour.) Merr pada membran korio alantois (CAM) embrio ayam, Majalah Farmasi Indonesia, 17(1):50-55

Kleinsmith, L.J., Kerrigan, D, Kelly., 1999, Angiogenesis,http://press2.nci.nih.gov/sciencebehing/angiogenesis/angio00.html, (diakses bulan Agustus 2008)

Knighton, D., D. Ausprunk, D.Tapper, and J. Folkman, 1977, Avascular and Vascular Phases of Tumor Grrowth in The Chick Embrio. Br.J. Cancer 35 no. 347-356

Mustofa dan Qomariah N., 2004, Aktivitas Antiplasmodial in vitro dan Sitotoksik Akar Pasak Bumi terhadap malaria di Kalimantan Selatan, Medika, 3, 147-152

Nurhanan M.Y., Hawariah L.P.A, Ilham A.M., Shukri, M.A.M, 2005, Cytotoxic Effects of the Root Extracts of Eurycoma longifolia, Jack,Phytother. Res. 19:994-996

Nurkhasanah and Pihie A.H.L., 2006, Apoptotic Cell Death Induced by Eurycomanone (Eurycoma longifolia, Jack) in Human Cervical Carcinoma Cells, Proceeding of International Conference on Mathematics and Natural Sciences

Ribatti, D., Gulandaris, A.,Bastaki, M., Vacca,A., Lurlalo, M., Roncali, L., Presta, M.,1997, New Model for Study of angiogenesis and Antiangiogenesis in The Chick Embryo Chorioallantoic Membrane: The Gelatin Sponge/ Chorioallantoic Membrane Assay, J Vasc Res, 34: 455-463

Ribbati, D., Vacca, A., Presta, M., 2002, The discovery of angiogenic factors: A historical review, General Pharmacology., 35:227-231

Sengupta, S., Toh S.A., Sellers L.A., Skepper, J.N., Koolwijk, P., Leung, H.W., Yeung H.W., Wong, R.N.S., Sasisekharan R., Fan, T.P.D, 2004, Modulating Angiogenesis : The Yin and the Yang in Ginseng, Circulation. 110:1219-1225

Sun, X., Ding, Y., Yan, X., Wu, L., Li, Q., Ceng, N., Qiu, Y., Zhang, M., 2004, Angiogenic synergistic effect of basic fibroblast growth factor and vascular endothelial growth factor in an in vitro quantitative microcarrier-based three-dimensional fibrin angiogenesis system, World J Gastroenterol 2004; 10(17): 2524-2528



DOI: https://doi.org/10.22146/tradmedj.8062

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