CYTOTOXIC ACTIVITY OF FRACTION n-HEKSANA : CHLOROFORM FROM METHANOLIC EXTRACT OF Rhizopora mucronata STEM BARKS ON MYELOMA CELL-LINES

https://doi.org/10.22146/tradmedj.8069

Harwoko Harwoko(1*), Esti Dyah Utami(2)

(1) Department of Pharmacy, University of Jenderal Soedirman
(2) Department of Pharmacy, University of Jenderal Soedirman
(*) Corresponding Author

Abstract


The Mangrove plants have been long used for traditional medicine to cure gastroenteritis and cancer theraphy. Rhizopora mucronata, one species of Mangrove, is still rare studied that have potention as anti cancer. This research goals was to fractination bioactive compound of R. mucronata with various organic solvent and cytotoxicity test of those fraction n-heksana : chloroform to cancer cell myeloma. The extract from R. mucronata stem barks was made by maseration with methanol. The extracts was partitioned with chloroform, etil acetate, and methanol. The fraction of chloroform was fractination with n-heksana : chloroform (3 : 2). The fraction respectively was examined their cytotoxicity on Myeloma cell-line by MTT methods.  Result showed that fraction n-heksana : chloroform from methanolic extract of R. mucronata stem barks was toxic on Myeloma cell, with the IC50 value was 15 µg/mLand phytochemical study showed that the active fractions contained flavonoid and terpenoid.

Keywords


Rhizopora mucronata; cytotoxic; myeloma;



References

Achmad, S. A., 2004, Kimia Bahan Alam, Suatu Pendekatan untuk Memahami Potensi Keanekaragaman Hayati dalam Bioindustri, Prosiding Seminar Nasional, Universitas Airlangga, Surabaya, 5 September 2004, 1- 25.

DepKes R.I., 2005, Profil Kesehatan Indonesia, Depkes R.I Jakarta, 21-25

Diastuti, H., Warsinah, Purwati, 2008, Isolasi Senyawa Bioaktif pada Tanaman Rhizopora mucronata sebagai Bahan Antikanker, Laporan Penelitian, Lembaga Penelitian UNSOED, Purwokerto.

Fehrman, F. dan Laimins, L.A., 2003, Human Papilloma Viruses; Targetting Diferentiating Ephitel Cells for Malignant Tranformation, Oncogen, 22: 5201-5207.

Gibbs J. B., 2000, Anticancer Drugs Targets, Growth Factors and Growth Factor Signaling, J Clinvers, 105 (1): 9- 13.

Hadiyati, T., 2000, Zonasi Mangrove Pada Daerah Akresi dan Non Akresi di Segara Anakan Cilacap, Biosfera, A Scientific Journal. Vol 17

Ma’at, S., 2004, Obat Tradisional untuk Pelayanan Kesehatan Formal. Prosiding Seminar Nasional , Universitas Airlangga, Surabaya, 5 September 2004, 45-49.

Soetarno, S., 2000, Potensi dan Manfaat Tumbuhan Mangrove sebagai Sumber Bahan Bioaktif, Acta Pharmaceutica Indonesia, 12 (4): 84- 103.

Warsinah dan Yulia, 2007, Fraksinasi Ekstrak Metanol Kulit Batang Rhizopora mucronata dan Uji Daya Hambatnya terhadap Bakteri Escherichia coli, Majalah Molekul.



DOI: https://doi.org/10.22146/tradmedj.8069

Article Metrics

Abstract views : 1022 | views : 4557

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2010 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada