OPTIMATION OF GREEN TEA WASTE AXTRACTION USING MICROWAVE ASSISTED EXTRACTION TO YIELD GREEN TEA EXTRACT

https://doi.org/10.22146/tradmedj.8088

Dwi Handayani(1*), Abdul Mun’im(2), Anna S. Ranti(3)

(1) Faculty of Pharmacy, University of Indonesia
(2) Faculty of Pharmacy, University of Indonesia
(3) Faculty of Pharmacy, University of Indonesia
(*) Corresponding Author

Abstract


Indonesia has a great wealth of biodiversity, but unfortunately the natural resources has been little studied and used in the therapy. One of the plants, grown in Indonesia is tea. Tea waste is waste from the tea industry, is quite a lot and has not been used optimally. This study aims to obtain the most effective green tea waste extraction conditions using MAE (Microwave Extraction Assisted) to produce green tea extract that has antioxidant optimum. From the research, the optimum MAE conditions are 450 W power, 60% ethanol, solid solvent comparison 1: 8 and 8 minutes extraction to produce extracts with optimum levels of polyphenols. Extracts obtained have moisture content of 7.83%, pH 5.5, and total polyphenols, total flavonoids, gallic acid, catechin, EGCG and caffeine respectively by 7.1%, 1.04%, 2.76%; 0.83%, 5.18% and 1.03%. Compared with the existing reference, extracts obtained qualify as cosmetic raw materials.


Keywords


antioxidants, extract green tea, green tea oil, green tea waste, MAE



References

Anesini, C., Ferraro, G. E., & Filip, R. 2008. Total Polyphenol Content and Antioxidant Capacity of Commercially Available Tea (Camellia sinensis) in Argentina. J.Agric.Food Chem., 56, 9225-9229

Badan Pengawas Obat dan Makanan. 2006. Monografi Ekstrak Tumbuhan Obat Indonesia (Vol. 2). Jakarta

Badan Pengawas Obat dan Makanan. 2010. Acuan Sediaan Herbal (1 ed. Vol. 5). Jakarta

Barel, A. O., Paye, M., & Maibach, H. I. 2001. Introduction. In A. O. Barel, M. Paye & H. I. Maibach (Eds.), Handbook of Cosmetic Science and Technology (pp. 1-2). New York: Marcel Dekker, Inc

Cardoso-Ugarte, G. A., Juárez-Becerra, G. P., Sosa-Morales, M. E., & López-Malo, A. 2013. Microwave-Assisted Extraction of Essential Oils from Herbs. J. Microwave Power & Electromagnetic Energy, 47(1), 63-72.

Committe of Expert on Cosmetic Products. 2001. Plants in Cosmetics (Vol. II). Germany: Koeblin Fortuna Druck

Delazar, A., Nahar, L., Hamedeyazdan, S., & Sarker, S. D. 2012. Microwave-assisted extraction in natural products isolation. Methods Mol Biol, 864, 89-115

Departemen Kesehatan Republik Indonesia. 1989. Materia Medika Indonesia (Vol. V). Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan

Departemen Kesehatan Republik Indonesia. 1995. Farmakope Indonesia (IV ed.). Jakarta

E.Reich, A.Schibli, Widmer, V., Jorns, R., Wolfram, E., & Debatt, A. 2006. HPTLC Methods for the Identification of Green Tea and Green Tea Extract. J.Liq.Chromatogr.&Rel.Tech., 14, 2141-2151

Eskilsson, C. S., & Bjorklund, E. 2000. Analytical-scale microwave-assisted extraction. J Chromatogr A, 902(1), 227-250

Etoh, H., Ohtaki, N., Kato, H., Kulkarni, A., & Morita, A. 2010. Sub-critical water extraction of residual green tea to produce a roasted green tea-like extract. Biosci Biotechnol Biochem, 74(4), 858-860

Higashi-Okai, K., Yamazaki, M., Nagamori, H., & Okai, Y. 2001. Identification and antioxidant activity of several pigments from the residual green tea (Camellia sinensis) after hot water extraction. J UOEH, 23(4), 335-344

Jain, T., Jain, V., Pandey, R., Vyas, A., & Shukla, S. 2009. Microwave Assisted Extraction for Phytoconstituents – An Overview. Asian J. Research Chem, 2 (1), 19-25

Kaur, C. D., & Saraf, S. 2011. Photochemoprotective Activity of Alcoholic Extract of Camellia sinensis. Int. J. Pharmacol., 7 (3), 400-404

Khalaf, N. A., Shakya, A. K., AL-Othman, A., EL-Agbar, Z., & Farah, H. 2008. Antioxidant Activity of Some Common Plants. Turk. J. Biol., 32, 51-55

Krisnan, R. 2004. Pengaruh Pemberian Ampas Teh (Camellia sinensis) Fermentasi dengan Aspergillus niger pada Ayam Broiler. JITV, 10(1).

Li DC, & Jiang JG. 2010. Optimization of The Microwave-Assisted Extraction Conditions of Tea Polyphenols from Green Tea. Int.J.Food Sci.Nutr., 61(8), 837-845

Mahmood, T., Akhtar, N., & Khan, B. A. 2010. The Morphology, Characteristics, and Medicinal Properties of Camellia sinensis’ Tea. J. Med. Plant. Res., 4(19), 2028-2033

Mahmood, T., Akhtar, N., Khan, B. A., Shoaib Khan, H. M., & Saeed, T. 2011. Changes in skin mechanical properties after long-term application of cream containing green tea extract. Aging Clin Exp Res, 23(5-6), 333-336

Mandal, V., Mohan, Y., & Hemalatha, S. 2007. Microwave Assisted Extraction - An Innovative and Promising Extraction Tool for Medicinal Plant Research. Phcog Rev., 1(1), 7-18

Marinova, G., & Batchvarov, V. 2011. Evaluation of the Methods for Determination of the Free Radical Scavenging Activity by DPPH Bulgarian J. Agri. Sci., 17(1), 11-24

Namita, P., Mukesh, R., & Vijay, K. J. 2012. Camellia Sinensis (Green Tea): A Review. Global Journal of Pharmacology, 6(2), 52-59

Oboh, H. A., & Omoregie, I. P. 2011. Total Phenolic and Antioxidant Capacity of Some Nigerian Beverages. Nigerian J. of Basic and App. Sci, 19 (1), 68-75

Pepe, R. C., Wenninger, J. A., & McEwen, G. N. (Eds.). 2002. International Cosmetic Ingredient Dictionary and Handbook (9 ed. Vol. 1). Washington Cosmetic, Toiletry and Fragrance Association

Quan, P. T., Hang, T. V., Ha, N. H., De, N. X., & Tuyen, T. N. 2006. Microwave-Assisted Extraction of Polyphenols From Fresh Tea Shoot. Sci. & Tech. Develop., 9(8), 69-75

Rence, B. W. 1994. Microwave Assisted Extraction. American Laboratory, RE14, 34-39

Saito, S. T., Welzel, A., Suyenaga, E. S., & Bueno, F. 2006. A Method for Fast Determination of Epigallocatechin Gallate (EGCG), Epicatechin (EC), Catechin (C) and Caffeine (CAF) in Green Tea Using HPLC. Ciênc. Tecnol. Aliment., 26(2), 394-400

Saqifah, N., Purbowati, E., & Rianto, E. 2010. Pengaruh Ampas Teh dalam Pakan Konsentrat terhadap Konsentrasi VFA dan NH3 Cairan Rumen untuk Mendukung Pertumbuhan Sapi Peranakan Ongole. Paper presented at the Seminar Nasional Teknologi Peternakan dan Veteriner

Septina, N. R. 2008. Analisis Tingkat Kepuasan dan Loyalitas Konsumen terhadap Minuman Teh Siap Minum (Ready to Drink) Merek Teh Botol Sosro di Jakarta Timur. from IPB: http://repository.ipb.ac.id

Sharma, A., Gupta, S., Sarethy, I. P., Dang, S., & Gabrani, R. 2012. Green tea extract: Possible mechanism and antibacterial activity on skin pathogens. Food Chem, 135(2), 672-675

Silverberg, J. I., Jagdeo, J., Patel, M., Siegel, D., & Brody, N. 2011. Green tea extract protects human skin fibroblasts from reactive oxygen species induced necrosis. J Drugs Dermatol, 10(10), 1096-1101

Simon. 2009. Pengaruh Pemberian Ampas Teh (Camellia sinensis) dalam Pakan Terhadap Analisis Usaha Domba Lokal Jantan Lepas Sapih Selama Tiga Bulan Penggemukan. Universitas Sumatera Utara, Medan

Thiele, J. J., & Dreher, F. 2005. Antioxidant Defense Systems in Skin. In P. Elsner & H. I. Maibach (Eds.), Cosmeceuticals and Active Cosmetics: Drugs Versus Cosmetics (Vol. 27, pp. 65-66). Boca Raton: Taylor & Francis Group

Xuejun Pan, Guoguang Niu, & Huizhou Liu. 2003. Microwave-Assisted Extraction of Tea Polyphenols and Tea Caffeine from Green Tea Leaves. Chem. Engineering & Processing, 42, 129-133

Yoo, K. M., Lee, C. H., Lee, H., Moon, B. K., & Lee, C. Y. 2008. Relative Antioxidant and Cytoprotective Activities of Common Herbs. Food Chemistry, 106, 929-936

Zuhud, E. A. M. 2011. Peran Sumber Daya Hutan dalam Mendukung Pengembangan Agro-Industri Djamoe. Paper presented at the Workshop Nasional Jamu dan Kosmetik

Zuo, Y., Chen, H., & Deng, Y. 2002. Simultaneous Determination of Catechins, Caffeine and Gallic Acids in Green, Oolong, Black and Pu-erh Tea Using HPLC with a Photodiode Array Detector. Talanta, 57, 307-316



DOI: https://doi.org/10.22146/tradmedj.8088

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