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THE COMPARISON OF EXTRACTION METHOD AND SOLVENT VARIATION ON YIELD AND ANTIOXIDANT ACTIVITY OF Brassica oleracea L. var. capitata f. rubra EXTRACT
https://doi.org/10.22146/tradmedj.8090 Rima Yulia Senja(1*), Elisa Issusilaningtyas(2), Akhmad Kharis Nugroho(3), Erna Prawita Setyowati(4)
(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Red Cabbage (Brassica oleracea L. Var. Capitata f. rubra) has a high anthocyanin content therefore it can be potential as a natural antioxidant. In this research, the antioxidant activity of the red cabbage extract was evaluated quantitatively with a spectroscopy method using DPPH reagent to obtain the value of IC50 . This research was divided into 2 stages. First, the influence of solvent variant to the extract yield of red cabbage powder and the maximum wavelength (λ) using a maceration protocol. The kind of solvent (70%, 80%, 95%, and 96% of ethanol) with addition 3% of citric acid. Second, the influence of extraction methods in neutral condition to the antioxidant activity. The results show the red cabbage powder maceration with 96% ethanol solvent (acid condition) exhibits the highest yield. The fresh red cabbage soxhletation with 96% ethanol (neutral condition) exhibits 288,5 nm maximum wavelength (λ) and 168,78 mg/mL of IC50 value.
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DOI: https://doi.org/10.22146/tradmedj.8090
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Faculty of Pharmacy
Universitas Gadjah Mada