.png)
.png)
* Corresponding Author
THE COMPARISON OF EXTRACTION METHOD AND SOLVENT VARIATION ON YIELD AND ANTIOXIDANT ACTIVITY OF Brassica oleracea L. var. capitata f. rubra EXTRACT
https://doi.org/10.22146/tradmedj.8090 Rima Yulia Senja(1*), Elisa Issusilaningtyas(2), Akhmad Kharis Nugroho(3), Erna Prawita Setyowati(4)
(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Red Cabbage (Brassica oleracea L. Var. Capitata f. rubra) has a high anthocyanin content therefore it can be potential as a natural antioxidant. In this research, the antioxidant activity of the red cabbage extract was evaluated quantitatively with a spectroscopy method using DPPH reagent to obtain the value of IC50 . This research was divided into 2 stages. First, the influence of solvent variant to the extract yield of red cabbage powder and the maximum wavelength (λ) using a maceration protocol. The kind of solvent (70%, 80%, 95%, and 96% of ethanol) with addition 3% of citric acid. Second, the influence of extraction methods in neutral condition to the antioxidant activity. The results show the red cabbage powder maceration with 96% ethanol solvent (acid condition) exhibits the highest yield. The fresh red cabbage soxhletation with 96% ethanol (neutral condition) exhibits 288,5 nm maximum wavelength (λ) and 168,78 mg/mL of IC50 value.
Keywords
Full Text:
pdf (Bahasa Indonesia)References
Chun, O.K., Kim, D.O. dan Lee, C.Y. 2003, Superoxide Radical Scavenging Activity of The Major Polyphenol in Fresh Plums, J. Food Chem, 51: 8067-8072
Dalimartha, S. dan Soedibyo, M. 1999, Awet Muda Dengan Tumbuhan Obat dan Diet Suplemen.,Trubus Agriwidya, Jakarta. hal. 36-40
Harborne J.B. 1987, Phytochemistry Methods, John Wiley and Sons: New York
Kristiana, H.D., Ariviani, S., Khasanah, L.U. 2012. Ekstraksi Pigmen Antosianin Buah Senggani (Melastoma malabathricum Auct. non Linn) dengan Variasi Jenis Pelarut, J.Teknosains Pangan 1(1) : 105-109
Lin, W., Wu R.T., Wu, T., Khor, T., Wang, H. dan Kong, A. 2008, Sulforaphane Suppressed LPS-induced Inflammation in Mouse Peritoneal Macrophages through Nrf2 Dependent Pathway, Biochem Pharmacol, 76(8) : 1776-1783
Mackiewicz, Z., Rimkevicius, A. 2008, Skin Aging. Institute of Experimental and Clinical Medicine at Vilnius University.
Neelufar. S., Alekhya, T. dan Sudhakar, K. 2012, Pharmacognostical and Phytochemical evaluation of Brasicca Oleracea Linn Var. Capitata F. Rubra (The Red Cabbage), J. pharm bio, 2(2) : 43-46.
Pokorny, J., Yanishlieva, N., and Gordon, M. 2001, Antioxidant in Food: Practical Application, CRC Press Cambridge, New York
Prior, R.L., Wu, X dan Schaich, K. 2005, Standardized methods for determination of antioxidant capacity and phenolich in foods and dietary supplement, J.Agric.Food Chem, 55 : 2698A-J
Rohdiana, D.(2001). Aktivitas Daya Tangkap Radikal Polifenol Dalam Daun Teh, Majalah Jurnal Indonesia 12 (1) : 53-58
Supiyanti,W., Wulansari, E.D., dan Kusmita, L. 2010, Uji aktivitas antioksidan dan penentuan kandungan antosianin total kulit buah manggis (Garcinia mangosta. L). Majalah Obat Tradisional, 15(2) : 64-70
Sunarni,T., (2005). Aktivitas Antioksidan Penangkap Radikal Bebas Beberapa kecambah Dari Biji Tanaman Familia Papilionaceae, Jurnal Farmasi Indonesia 2 (2) : 53-61
Wirda, Z., Halim, H., Millati, T., Zulhidiani, R. 2011. Pengaruh Berbagai Jenis Pelarut dan Asam Terhadap Rendemen Antosianin Kubis Merah (Brassica oleraceae capitata). J. Agroscientic, 18: 2
DOI: https://doi.org/10.22146/tradmedj.8090
Article Metrics
Abstract views : 8926
| 
views : 160350
Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada