Eel Oil Attenuates Acetaminophen-Induced Acute Liver Injury Through Inhibition of Oxidative Stress in Rats

Heru Sasongko(1*), Renita Wahyu Nur Hidayati(2), Bayu Anggoro Saputro(3), Muhammad Fiqri Zulpadly Mahmud(4), Yeni Farida(5)

(1) *) Department of Diploma 3 Pharmacy, Vocational School, Universitas Sebelas Maret, Surakarta, Central Java; *) Department of Pharmacy, Faculty of Mathematics and Natural Science, Universitas Sebelas Maret, Surakarta, Central Java
(2) Department of Pharmacy, Faculty of Mathematics and Natural Science, Universitas Sebelas Maret, Surakarta, Central Java
(3) Department of Pharmacy, Faculty of Mathematics and Natural Science, Universitas Sebelas Maret, Surakarta, Central Java
(4) Department of Diploma 3 Pharmacy, Vocational School, Universitas Sebelas Maret, Surakarta, Central Java
(5) Department of Pharmacy, Faculty of Mathematics and Natural Science, Universitas Sebelas Maret, Surakarta, Central Java
(*) Corresponding Author


Health practitioners all over the world have studied liver injury caused by drug side effects. Excessive production of free radicals causes cell damage, which has implications for pathological conditions in both humans and animals. Omega-3 fatty acids are a component of fish that can work as hepatoprotective agents. Eel (Anguilla bicolor) is known to contain omega-3 including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). This study aimed to evaluate the hepatoprotective activity of eel oil on rats via inhibiting oxidative stress. Methods: Acetaminophen-induced male Wistar rats were used as liver injury experimental models. Rats were divided into 5 groups, namely normal control, negative control, positive control (silymarin, 100 mg/kg), and two groups of eel oil dose (2000 mg/kg and 4000 mg/kg). The study was conducted for 14 days. The levels of serum glutamic pyruvic transaminase (SGPT), serum glutamic pyruvic transaminase (SGOT), alkaline phosphatase (ALP), total bilirubin, albumin, Malondialdehyde (MDA), and glutathione (GSH) levels of the liver organ were measured. The data were analyzed using statistics and analysis of variance. Results: The study showed that eel fish oil can reduce SGPT and total bilirubin levels of male Wistar rats induced by acetaminophen. Eel oil at a dose of 4000 mg/kg could significantly reduce SGPT and liver bilirubin levels in male Wistar rats (p<0.05). Eel oil is effective in reducing malondialdehyde (MDA) levels and increasing glutathione (GSH) levels at a dose of 2000 mg/kg. Conclusion: Eel oil has hepatoprotective activity by inhibiting SGPT, total bilirubin, MDA, and increasing GSH levels in rats.


Omega-3; Liver injury; Malondialdehyde; Glutathione

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Aflanie, I., Muhyi, R. & Suhartono, E., 2015. 'Effect of Heavy Metal on Malondialdehyde and Advanced Oxidation Protein Products Concentration: A Focus on Arsenic, Cadmium, and Mercury'. JOMB 4, 332–337.

Asao, T. & Asaduzzaman, M., 2018. 'Phytochemicals: Source of Antioxidants and Role in Disease Prevention'. BoD – Books on Demand.

Atta, E., Mohamed, N. & Abdelgawad, A., 2017. 'Antioxidants: An Overview on the Natural and Synthetic Types'. European Chemical Bulletin 6, 365.

Balasaheb Nimse, S., Pal, D., 2015. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Advances 5, 27986–28006.

Blondet, N.M., Messner, D.J., Kowdley, K.V. & Murray, K.F., 2018. Chapter 43 - 'Mechanisms of Hepatocyte Detoxification, in: Said, H.M. (Ed.), Physiology of the Gastrointestinal Tract (Sixth Edition)'. Academic Press, pp. 981–1001.

Dhage, P.A., Prajakta, A., Archana, A. & Sharbidre, 2021. 'Bimodal Behavior of Ascorbic Acid in Musca domestica Larvae'. Biointerface Res Appl Chem 12, 5199–5216.

Di Minno, M.N.D., Russolillo, A., Lupoli, R., Ambrosino, P., Di Minno, A. & Tarantino, G., 2012. 'Omega-3 fatty acids for the treatment of non-alcoholic fatty liver disease'. World J Gastroenterol 18, 5839–5847.

DiNicolantonio, J.J. & OKeefe, J., 2019. 'Importance of maintaining a low omega-6/omega-3 ratio for reducing platelet aggregation, coagulation and thrombosis'. Open Heart 6, e001011.

Du, K., Ramachandran, A. & Jaeschke, H., 2016. 'Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential'. Redox Biology 10, 148–156.

Ellman, G.L., 1959. 'Tissue sulfhydryl groups. Archives of biochemistry and biophysics' 82, 70–77.

Farid, A.S., Shemy, M.A.E., Nafie, E., Hegazy, A.M. & Abdelhiee, E.Y., 2019. 'Anti-inflammatory, anti-oxidant and hepatoprotective effects of lactoferrin in rats'. Drug and Chemical Toxicology 0, 1–8.

Freitag, A.F., Cardia, G.F.E., da Rocha, B.A., Aguiar, R.P., Silva-Comar, F.M. de S., Spironello, R.A., Grespan, R., Caparroz-Assef, S.M., Bersani-Amado, C.A. & Cuman, R.K.N., 2015. 'Hepatoprotective Effect of Silymarin (Silybum marianum) on Hepatotoxicity Induced by Acetaminophen in Spontaneously Hypertensive Rats'. Evid Based Complement Alternat Med 2015, 538317.

He, J., Bai, K., Hong, B., Zhang, F. & Zheng, S., 2017. 'Docosahexaenoic acid attenuates carbon tetrachloride-induced hepatic fibrosis in rats'. International Immunopharmacology 53, 56–62.

Iraz, M., Erdogan, H., Ozyurt, B., Ozugurlu, F., Ozgocmen, S. & Fadillioglu, E., 2005. 'Brief communication: omega-3 essential fatty acid supplementation and erythrocyte oxidant/antioxidant status in rats'. Ann Clin Lab Sci 35, 169–173.

Kusharto, C.M., Widyasari, R.A.H.E., Budywiryawan, Wiyono, E.S. & Sugengherisuseno, 2014. 'Nutritive Value and Fatty Acids Profile of Fresh Indonesian Eel (Anguilla bicolor) and Kabayaki'. Jurnal Sains Kesihatan Malaysia, 12, 41-46.

Nobili, V., Bedogni, G., Alisi, A., Pietrobattista, A., Risé, P., Galli, C. & Agostoni, C., 2011. 'Docosahexaenoic acid supplementation decreases liver fat content in children with non-alcoholic fatty liver disease: double-blind randomised controlled clinical trial'. Archives of disease in childhood 96, 350–353.

Novianto, A., Nurrochmad, A., Puspita Sari, I. & Astuti, P., 2015. 'Hepatoprotective effect of combination of curcuma domestica val and Phyllanthus niruri linn against paracetamol-induced liver damage in wistar rats'. International Journal of Pharmaceutical and Clinical Research 7, 450–457.

Ntamo, Y., Ziqubu, K., Chellan, N., Nkambule, B.B., Nyambuya, T.M., Mazibuko-Mbeje, S.E., Gabuza, K.B., Marcheggiani, F., Tiano, L. & Dludla, P.V., 2021. 'Drug-Induced Liver Injury: Clinical Evidence of N-Acetyl Cysteine Protective Effects'. Oxidative Medicine and Cellular Longevity 2021, e3320325.

Pineda-Peña, E.A., Martínez-Pérez, Y., Galicia-Moreno, M., Navarrete, A., Segovia, J., Muriel, P., Favari, L., Castañeda-Hernández, G. & Chávez-Piña, A.E., 2018. 'Participation of the anti-inflammatory and antioxidative activity of docosahexaenoic acid on indomethacin-induced gastric injury model'. European Journal of Pharmacology 818, 585–592.

Qiu, Y.-D., Wang, S., Yang, Y. & Yan, X.-P., 2012. 'Omega-3 polyunsaturated fatty acids promote liver regeneration after 90% hepatectomy in rats'. World J Gastroenterol 18, 3288–3295.

Sasongko, H., Efendi, N.R., Budihardjo, A., Farida, Y., Amartiwi, T., Rahmawati, A.A., Wicaksono, A. & Sugiyarto, 2017. 'Solvent and extraction methods effects on the quality of eel ( Anguilla bicolor ) oil'. J. Phys.: Conf. Ser. 795, 012021.

Sasongko, H., Pratiwi, D., Amartiwi, T., Efendi, N.R. & Sugiyarto, 2019. 'Hepatoprotective Effect of Mountain Papaya (Vasconcellea pubescens A.DC.) Fruit Extract against Acetaminophen-Induced Acute Liver Damage'. Presented at the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development, pp. 66–70.

Sasongko, H. & Sugiyarto, S., 2018. 'Pengaruh Pemberian Ekstrak Daun Karika (Vasconcellea pubescens A.DC.) Terhadap Nilai SGPT dan SGOT pada Tikus Jantan yang Diinduksi Paracetamol'. JPSCR : Journal of Pharmaceutical Science and Clinical Research 3, 70–75.

Setyawati, I., 2018. 'Effectiveness of Sweet Orange Peel Extract (Citrus sinensis) on The Improvement of Liver Functions of Animal Trials Induced by Cigarette Smoke'. Journal of Young Pharmacists 10, S132–S135.

Simeonova, R., Stavrakov, G., Philipova, I., Atanasova, M. & Doytchinova, I., 2020. 'Antioxidant Activity of a Novel Acetylcholinesterase Inhibitor: In Vivo and Ex vivo Studies (preprint)'. Medicine & Pharmacology.

Singal, R., Singal, R.P., Sandhu, K., Singh, B., Bhatia, G., Khatri, A. & Sharma, B.P., 2015. 'Evaluation and comparison of postoperative levels of serum bilirubin, serum transaminases and alkaline phosphatase in laparoscopic cholecystectomy versus open cholecystectomy'. J Gastrointest Oncol 6, 479–486.

Sprynskyy, M., Monedeiro, F., Monedeiro-Milanowski, M., Nowak, Z., Krakowska-Sieprawska, A., Pomastowski, P., Gadzała-Kopciuch, R. & Buszewski, B., 2022. 'Isolation of omega-3 polyunsaturated fatty acids (eicosapentaenoic acid - EPA and docosahexaenoic acid - DHA) from diatom biomass using different extraction methods'. Algal Research 62, 102615.

Tzankova, V., Aluani, D., Kondeva-Burdina, M., Yordanov, Y., Odzhakov, F., Apostolov, A. & Yoncheva, K., 2017. 'Hepatoprotective and antioxidant activity of quercetin loaded chitosan/alginate particles in vitro and in vivo in a model of paracetamol-induced toxicity'. Biomedicine & Pharmacotherapy 92, 569–579.

Vargas-Mendoza, N., Madrigal-Santillán, E., Morales-González, A., Esquivel-Soto, J., Esquivel-Chirino, C., García-Luna Y González-Rubio, M., Gayosso-de-Lucio, J.A. & Morales-González, J.A., 2014. 'Hepatoprotective effect of silymarin'. World J Hepatol 6, 144–149.

Wu, C.T., Deng, J.S., Huang, W.C., Shieh, P.C., Chung, M.I. & Huang, G.J., 2019. 'Salvianolic Acid C against Acetaminophen-Induced Acute Liver Injury by Attenuating Inflammation, Oxidative Stress, and Apoptosis through Inhibition of the Keap1/Nrf2/HO-1 Signaling'. Oxidative Medicine and Cellular Longevity, 1–13.


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