ANTIINFLAMATORY ACTIVITY OF METHANOLIC EXTRACT OF Mangifera casturi IN THIOGLYCOLLATE-INDUCED LEUKOCYTE MIGRATION ON MICE

https://doi.org/10.22146/tradmedj.8217

Nanang Fakhrudin(1*), Peni Susilowati Putri(2), Sutomo Sutomo(3), Subagus Wahyuono(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Mathematic and Science, Universitas Lambung Mangkurat
(3) Faculty of Mathematic and Science, Universitas Lambung Mangkurat
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Kasturi (Mangifera casturi) is a plant originally from Kalimantan, mainly in South Kalimantan. Although there is no report regarding the usage of the fruit in traditional healings, current research demonstrate that methanolic extract of Mangifera casturi fruit demonstrated high antioxidant activity. As oxidant plays crucial role in inflammatory related diseases, we investigate the antiinflammatory effect of Mangifera casturi fruit methanolic extract. To assess the antiinflammatory activity, we used thioglycollate-induced leukocyte migration in mice.  Male balb/c mice were pretreated (i.p) with Mangifera casturi fruit methanolic extract followed by thioglikolat (i.p) to induce leukocyte migration. After 4,5 hours, the mice were sacrificed and the number of leukocytes were counted using hemocytometer under light microscope.   Our experiment indicates that Mangifera casturi fruit methanolic extract significantly exhibited antiinflammatory activity by inhibiting the migration of leukocytes induced by thioglycollate. However, the activity of the extracts was lower than the positive control, indomethacin. Further purification was required to obtain the active compound with the activity comparable to indomethacin. Thin layer chromatography (TLC) analysis demonstrates that the methanolic extract of Mangifera casturi fruit contains terpenoid and phenolic compounds.


Keywords


Mangifera casturi, leukocyte migration, antiinflammation



References

Baratawidjaja, K. G., dan Rengganis, I., 2010, Imunologi Dasar, (Edisi ke-9), Jakarta: Balai Penerbit FKUI

Calixto, J.B., Otuki, M.F., dan Santos, A.R.S., 2003, Anti-inflamatory Compound of Plant origin, Planta Med., 69: 973-983

Eming, S. A., Krieg, Thomas, dan Davidson, J. M., 2007, Inflammation in Wound Repair: Molecular and Cellular Mechanism, Journal of Investigative Dermatology, 27

Morcillo, Esteban J., Estrela, J., dan Cortijo, J., 1990, Oxidative Stress And Pulmonary Inflammation: Pharmacological Intervention With Antioxidants, Pharmacological Research, 40 (5): 393-404

Mustikasari, K. dan Ariyani, D., 2007, Skrining Metabolit Sekunder Pada Akar Binjai (Mangifera caesia) dan kasturi (Mangifera casturi). Laporan Penelitian Dosen. Banjarbaru: FMIPA Universitas Lambung Mangkurat

Ravipati, A. S., Zhang, L., Koyyalamudi, S. R., Jeong, S. C., Reddy, N., Barlett, J., Smith, P. T., Shanmugam, K., Munch, G., Wu, M. J., dan Manavalan, 2012, Antioxidant and Antiinflamatory Activities of Selected Chinese Medical Plant and Their Relation with Antioxidant Content, BioMed. central

Segal, B.H., Kuhns, D.B., Ding Li, Gallin J. I., dan Holland S.M., 2002, Thioglicollate peritonitis in mice lacking C5, 5-lipoxygenase, or p47phox:complement, leukotrienes, and reactive oxidants in acute inflammation, Journal of Leukocyte Biology,. 71

Suhartono, E., Viani, E., Ramadhan M. A., Syahuri, I. G., Rakhman M. F., dan Indrawardhana, D., 2012, Total Flavonoid and Antioxidant Activity of Some Selected Medicinal Plants in South Kalimantan of Indonesia. ACPBEE Proced. Singapore

Sutomo, Wahyuono, S., Rianto, S., dan Setyowati, E.P., (2013a). Isolation and Identification of Active Compound of n-hexane Fraction from Kasturi (Mangifera casturi Konsterm.) against Antioxidant and Immunomodulatory Activity, Journal of Biological Sciences, 13: 596-506

Sutomo, Wahyuono, S., Rianto, S., dan Setyowati, E. P., 2013b, Antioxidant activity assay of extracts and active fraction of kasturi fruit (Mangifera casturi Kosterm.) using DPPH Methodh. Laporan Penelitian. Yogyakarta:Fakultas Farmasi Universitas Gadjah Mada



DOI: https://doi.org/10.22146/tradmedj.8217

Article Metrics

Abstract views : 3017 | views : 5641

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada