Mahfudz L. D.(1*), R. Murwani(2), Kusumowardani T. H.(3), D. X. Hou(4)

(1) Faculty of Animal Sciences, Diponegoro University
(2) Faculty of Animal Sciences, Diponegoro University
(3) Faculty of Animal Sciences, Diponegoro University
(*) Corresponding Author


The present study was to aimed to evaluated and compare in vitro antioxidant activities of 2 Indonesian herbal ingredients (A and B), determined total phenol content., cytotoxic and apoptosis induction activities on HL-60 cells. These data were providing some useful information for people healthy dietary and the new potential application of natural antioxidant containing food materials in functional foods and also as new cancer therapeutics promising candidates. The parameters were total antioxidant activity, amount of total cytotoxic effect on the growth of human promyelocytic leukemia cells (HL-60). Statistical comparison was perform with Student’s t-test at p<0.05. The correlation coefficient (r2) between the parameters tested was established by regression analysis. The scavenging effect of extracts herbal on DPPH radicals increased from 0.3-1.5 mg/ml, where is sample A 14.33% to 64.29% and sample B 9.09% to 57.53% was obtained. High content of total phenol compounds were in sample A (21.72 mg GAE/g), lower amounts were in sample B (17.53 mg GAE/g). Apoptosis of HL-60 cells from the morphological changes side (chromatin condensation). Chromatin condensation, a specific and distinct feature of apoptotic cells, was found in the majority of treated cells. The results indicated that the cell death receptor pathway was involved in the apoptosis induced by Indonesian herbal extracts.


Indonesian herbal, antioxidant, apoptosis of HL-60 cells

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