Synergism of Anti-Malarial Effect of Carica papaya L. and Moringa oleifera Leaf Extracts in Mice

https://doi.org/10.22146/mot.86703

Yenni Yusuf(1*), Rangga Meidianto Asri(2), Rivaldo Go(3), Muhammad Naufal Zuhair(4), Kania Meliani Kaharuddin(5), Martrisna Dara Karnia Parenden(6), Mesakh Diki Saputra(7), Diany Elim(8), Muhammad Fadhil Banjar(9), Hartono Hartono(10)

(1) Department of Parasitology, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi
(2) Pharmacy Science and Technology Department, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi
(3) Undergraduate School, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi
(4) Undergraduate School, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi
(5) Undergraduate School, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi
(6) Undergraduate School, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi
(7) Undergraduate School, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi
(8) Undergraduate School, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi
(9) Undergraduate School, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi
(10) Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar, South Sulawesi
(*) Corresponding Author

Abstract


Malaria parasites, Plasmodium spp, can develop resistance to anti-malarial drugs. Hence, potential alternative therapeutic agents should be explored. This research aimed to investigate the anti-malarial effect of a combination of Carica papaya and Moringa oleifera leaf extract. Peter’s test performed on Balb/c mice to investigate the malarial parasite growth inhibition. Mice were infected with Plasmodium berghei by intraperitoneal injection to provoke rodent malaria. After infection, we grouped every 5-6 mice into different treatment groups, including a negative control group, a positive control group treated with artesunate, a moringa group, a papaya group, and a combination therapy group. Treatments were initiated 3 hours after infection (day 0) and continued every day until day 3. On day 4, we examined a thin smear of the tail vein blood for parasitemia to calculate the suppression rate. The result shows that the parasite suppression rate of C. papaya leaf extract (CPLE) is 15.02 % (percent parasitemia 4.56±1.96), the suppression rate of M. oleifera leaf extract (MOLE) is 17.32% (percent parasitemia 4.44±1.78) and that of the combination extract (CE) is 28.73% (percent parasitemia 3.82±1.48).  In conclusion, there is a synergism of the anti-malarial effect of the combined leaf extract of C. papaya and M. oleifera.


Keywords


Anti-malarial drugs; Carica papaya L.; Moringa oleifera; Parasite suppression; Peter’s test; Plasmodium berghei

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DOI: https://doi.org/10.22146/mot.86703

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