Phytochemical Analysis and Anti-Collagenase Activity of Celery (Apium graveolens L.) Extract

https://doi.org/10.22146/mot.91756

Fahrizal Amanda Permana(1), Meitha Khansa Khairunnisa(2), Purwanto Purwanto(3), Sri Handayani(4), Riris Istighfari Jenie(5*)

(1) Undergraduate Program, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, DIY
(2) Undergraduate Program, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, DIY
(3) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, DIY
(4) Research Center for Food Technology and Processing, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Yogyakarta
(5) *) Laboratory of Macromolecule Engineering, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, DIY *) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, DIY
(*) Corresponding Author

Abstract


Aging is a normal process experienced by humans. One of the causes of aging is the body's intrinsic activity, such as the activity of one of the extracellular matrix-breaking enzymes, collagenase. Demand for skin care to prevent aging is increasing, especially plant-based skin care. This study aims to explore the potency of celery extract as an anti-collagenase. In this study, we used celery extract as a sample obtained from the maceration process by seventy percent ethanol 70% as the solvent. Sample compounds are then detected by LC-HRMS to determine the major compounds contained in a sample and their concentrations. Anti-collagenase activity was observed by enzymatic assay. The LC-HRMS analysis confirmed that apigenin, hispidulin, and catechin were included in the celery extract, with apigenin as the major compound of celery with AUC 73479.21×103, the AUC of hispidulin is 17063.21×103, and catechin 61.14×103. The percentage of collagenase inhibition of celery extract up until 200 μg/mL was 59.22% with an IC50 value of 132.48 µg/mL. These data indicated that celery containing apigenin, hispidulin, and catechin has an anti-collagenase activity worth further exploring for a healthy skin supplement.


Keywords


Anti-collagenase; Celery; Enzymatic Assay; LC-HRMS; Skin aging

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DOI: https://doi.org/10.22146/mot.91756

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