Exploring the Potential of NIR Spectroscopy and Chemometrics to Verify the Flavonoid Content in Litsea cubeba

https://doi.org/10.22146/mot.99185

Khrisna Agung Cendekiawan(1), Dhina Ayu Susanti(2), Nafisah Isnawati(3), Shinta Mayasari(4), Florentinus Dika Octa Riswanto(5*)

(1) *) Division of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Sanata Dharma University, Campus 3 Paingan Maguwoharjo Depok Sleman, Yogyakarta 55282 *) Pharmacy Study Program, Universitas dr. Soebandi, Jalan dr. Soebandi Nomor 99 Jember, East Java
(2) Pharmacy Study Program, Universitas dr. Soebandi, Jalan dr. Soebandi Nomor 99 Jember, East Java
(3) Pharmacy Study Program, Universitas dr. Soebandi, Jalan dr. Soebandi Nomor 99 Jember, East Java
(4) Pharmacy Study Program, Universitas dr. Soebandi, Jalan dr. Soebandi Nomor 99 Jember, East Java
(5) Division of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Sanata Dharma University, Campus 3 Paingan Maguwoharjo Depok Sleman, Yogyakarta 55282
(*) Corresponding Author

Abstract


The ancient records about herbal medicine show traditional healing practices are deeply rooted in Indonesian culture. The medical use of plants decreased because of inheritance customs combined with the lack of written documentation on medicinal plants but dependent on oral information transmission. Traditional research and preservation activities of local healing plants which belong to specific cultural communities become possible through this phenomenon. Researchers conducted ethnopharmacological research about antidiarrheal medicinal plant uses that included traditional knowledge about flavonoid-containing plants within the Osing ethnic group of West Java Indonesia. The goal of this research was to verify flavonoid compounds in Osing's medicinal plants through Near Infra-Red (NIR) spectroscopy combined with chemometric methods. The evaluation of antidiarrheal potential for these compounds relied on flavonoid standard reference substances because these compounds demonstrate strong antioxidant properties which stabilize intestinal functioning. Chemometric techniques together with Near Infra-Red spectroscopy (NIR) were applied to analyze krangean leaves (Litsea cubeba) in order to establish their flavonoid compound levels. The quality assessment of multivariate calibration models shows that PLS regression produces RMSEC of 2.870 and RMSEP of 0.0124 with R2cal = 0.9931 and R2val = 0.9919. The analysis using linear discriminant analysis (LDA) succeeded in its completion with recognition rates above 90% for each discriminating class.


Keywords


chemometrics; ethnopharmacy; krangean; NIR spectroscopy; Osing

Full Text:

PDF


References

Amat-Ur-rasool, H., Symes, F., Tooth, D., Schaffert, L.N., Elmorsy, E., Ahmed, M., Hasnain, S., Carter, W.G., (2020) Potential Nutraceutical Properties of Leaves from Several Commonly Cultivated Plants. Biomolecular, 10(11), 1556. https://doi:10.3390/BIOM10111556

Bevilacqua, M., Bro, R., Marini, F., Rinnan, Å., Rasmussen, M.A., Skov, T., (2017). Recent Chemometrics Advances for Foodomics. TrAC - Trends in Analytical Chemistry, 96, 42–51. https://doi:10.1016/J.TRAC.2017.08.011

Bhagawan, W.S., Suproborini, A., Prastya, D.L.P., Nurfatma, A., Putra, R.T., (2022). Ethnomedicinal Study, Phytochemical Characterization, and Pharmacological Confirmation of Selected Medicinal Plant on the Northern Slope of Mount Wilis, East Java, Indonesia. Biodiversitas; 23(8), 4303–4313. https://doi:10.13057/BIODIV/D230855

Brereton, R.G., Jansen, J., Lopes, J., Marini, F., Pomerantsev, A., Rodionova, O., Roger, J.M., Walczak, B., Tauler, R., (2018). Chemometrics in Analytical Chemistry—Part II: Modeling, Validation, and Applications. Analytical and Bioanalytical Chemistry, 410, 6691–6704. https://doi:10.1007/s00216-018-1283-4

Fakayode, S.O., Baker, G.A., Bwambok, D.K., Bhawawet, N., Elzey, B., Siraj, N., Macchi, S., Pollard, D.A., Perez, R.L., Duncan, A.V., (2020). Molecular (Raman, NIR, and FTIR) Spectroscopy and Multivariate Analysis in Consumable Products Analysis. Applied Spectroscopy Reviews, 55(8), 647–723. https://doi:10.1080/05704928.2019.1631176.

Farag, R.S., Abdel-Latif, M.S., Abd El Baky, H.H., Tawfeek, L.S., (2020). Phytochemical Screening and Antioxidant Activity of Some Medicinal Plants’ Crude Juices. Biotechnology Reports, 28, e00536. https://doi:10.1016/J.BTRE.2020.E00536

Gad, H.A., El-Ahmady, S.H., Abou-Shoer, M.I., Al-Azizi M.M., (2013). Application of Chemometrics in Authentication of Herbal Medicines: A Review. Phytochemistry Analysis; 24,1–24. https://doi:10.1002/pca.2378

Hair, J.F., Hult, G.T.M., Ringle, C.M., Sarstedt, M., Danks, N.P., Ray, S., (2021). Partial Least Squares Structural Equation Modeling (PLS-SEM) Using R, Springer, 197. https://10.1007/978-3-030-80519-7

Kusumo, D.W., Sulistiyowati, E.L., Rohmah, H., Melinda, N.A., (2023). Ethno-Pharmaceutical Study of Medicinal Plants for Care and Treatment of Wounds in Indonesia: Systematic Data Search and Preclinical Review. Journal Jamu Indonesia, 8, 1–9. https://doi:10.29244/JJI.V8I1.232

Luo, D., Shao, Y., (2013). Classification of Chinese Herbal Medicine Based on Improved LDA Algorithm Using Machine Olfaction. Applications of Mechanical Materials. 239–240, 1532–1536. https://doi:10.4028/www.scientific.net/AMM.239-240.1532

Nurani, L.H., Rohman, A., Windarsih, A., Guntarti, A., Riswanto, F.D.O., Lukitaningsih, E., Fadzillah, N.A., Rafi, M., (2021) Metabolite Fingerprinting Using 1H-NMR Spectroscopy and Chemometrics for Classification of Three Curcuma Species from Different Origins. Molecules, 26, 1–13. https://doi.org/10.3390/molecules26247626

Prasetyo, B., Chikmawati, T., Walujo, E.B., Amzu, E., (2018). Ethnoecology: The Traditional Landscape of Osing Tribe in Banyuwangi, Indonesia. Biodiversitas, 19(6), 2003–2009. https://doi:10.13057/BIODIV/D190604

Puspitasari, E., Damayanti, A.E., Zikrina, I.N.S., Dianasari, D., (2021). Exploring the Ethnopharmaceutical Plants of Osing Tribe in Banyuwangi Regency: Potential Application for COVID-19 Therapy. Journal of Ulum Islam, 33, 113–120. https://doi.org/10.33102/uij.vol33no3.330

Reyes-García, V. (2010). The Relevance of Traditional Knowledge Systems for Ethnopharmacological Research: Theoretical and Methodological Contributions. Journal of Ethnobiology and Ethnomedicine, 6, 1-12. https://doi.org/10.1186/1746-4269-6-32

Rinnan, Å., Berg, van den., Engelsen, S.B., (2009). Review of the Most Common Pre-Processing Techniques for near-Infrared Spectra. TrAC - Trends in Analytical Chemistry, 28, 1201–1222. https://doi:10.1016/j.trac.2009.07.007

Sima, I.A. Andrási, M. Sârbu, C. (2018). Chemometric Assessment of Chromatographic Methods for Herbal Medicines Authentication and Fingerprinting. Journal of Chromatography of Sciences, 56, 49–55. https://doi:10.1093/chromsci/bmx080

Singh, Y.D., Panda, M.K., Satapathy, K.B., (2020). Ethnomedicine for Drug Discovery. In Patra, J.K., Shukla, A.C., & Das, G., Advances in Pharmaceuticals Biotechnology (pp. 15–28). Elsevier Science. https://doi:10.1007/978-981-15-2195-9_2/

Sumarni, W., Sudarmin, S., Sumarti, S.S., (2019). The Scientification of Jamu: A Study of Indonesian’s Traditional Medicine. Journal of Physics Conference Series, 1321(3), 1–7. https://10.1088/1742-6596/1321/3/032057

Windarsih, A., Rohman, A., Irnawati., Riyanto, S., (2021). The Combination of Vibrational Spectroscopy and Chemometrics for Analysis of Milk Products Adulteration. International Journal of Food Sciences, 2021(3), 1-15. https://doi:10.1155/2021/8853358

Zaman, W., Ye, J., Saqib, S., Liu, Y., Shan, Z., Hao, D., Chen, Z., Xiao, P., (2021). Predicting Potential Medicinal Plants with Phylogenetic Topology: Inspiration from the Research of Traditional Chinese Medicine. Journal of Ethnopharmacology, 281, 114515. https://doi:10.1016/J.JEP.2021.114515



DOI: https://doi.org/10.22146/mot.99185

Article Metrics

Abstract views : 940 | views : 210

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2025 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada