Enzyme and hormone activities related to phosphorus uptake limitation in oil palm (Elaeis guineensis Jacq.)

Sigit Dwi Maryanto; Randi Abdur Rohman; Roberdi Roberdi; Tri Rini Nuringtyas; Purnomo Purnomo; Diah Rachmawati; Eka Tarwaca Susila Putra; Condro Utomo; Tony Liwang; Budi Setiadi Daryono

doi:10.22146/ijbiotech.109941

Enzyme and hormone activities related to phosphorus uptake limitation in oil palm (Elaeis guineensis Jacq.)

https://doi.org/10.22146/ijbiotech.109941

Sigit Dwi Maryanto⁽¹⁾, Randi Abdur Rohman⁽²⁾, Roberdi Roberdi⁽³⁾, Tri Rini Nuringtyas⁽⁴⁾, Purnomo Purnomo⁽⁵⁾, Diah Rachmawati⁽⁶⁾, Eka Tarwaca Susila Putra⁽⁷⁾, Condro Utomo⁽⁸⁾, Tony Liwang⁽⁹⁾, Budi Setiadi Daryono^(10*)

(1) Doctoral Program, Department of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia; Biotechnology Department, Plant Production and Biotechnology Division, PT SMART Tbk, Jl. Raya Cijayanti, Babakan Madang, Bogor 16810, West Java, Indonesia
(2) Biotechnology Department, Plant Production and Biotechnology Division, PT SMART Tbk, Jl. Raya Cijayanti, Babakan Madang, Bogor 16810, West Java, Indonesia
(3) Biotechnology Department, Plant Production and Biotechnology Division, PT SMART Tbk, Jl. Raya Cijayanti, Babakan Madang, Bogor 16810, West Java, Indonesia
(4) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
(5) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
(6) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
(7) Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281, Indonesia
(8) Biotechnology Department, Plant Production and Biotechnology Division, PT SMART Tbk, Jl. Raya Cijayanti, Babakan Madang, Bogor 16810, West Java, Indonesia
(9) Biotechnology Department, Plant Production and Biotechnology Division, PT SMART Tbk, Jl. Raya Cijayanti, Babakan Madang, Bogor 16810, West Java, Indonesia
(10) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract

Phosphorus (P) is an essential element for oil palm growth and development. Acid phosphatase (Apase) and Pti‐interacting serine/threonine kinase are two enzymes which enzymes confirmed to be related to P‐uptake in oil palm, therefore their activities in oil palm treated with P‐limitation need to be quantified. Acid phosphatase is believed to be induced by P‐deprivation. Conversely, the Pto‐interaction (Pti) serine/threonine kinase activity is associated with abiotic stress. The aim of this study was to quantify of activities of two selected enzymes and phytohormone content in oil palm‐clones in the P‐limitation condition. Two oil palms genotypes were treated with three P dosages i.e. 0% (v/v), 4.67% (v/v), and 14.02% (v/v) represented as starvation, deficiency, and optimum condition, respectively. The activity of these two enzymes was quantified in mitochondria and cytoplasm using spectrophotometry and modified dot‐blot methods, while abscisic acid, indole acetic acid and gibberellic acid content was quantified using ultra performance liquid chromatography (UPLC). The result showed that the Apase activity in P‐optimum was higher than starvation and deficiency in leaf and root tissues in both genotypes, whereas Pti serine/threonine kinase activity was higher in prolific than non‐prolific genotypes in P‐deficient dosage. Furthermore, abscisic acid content was higher in prolific than non‐prolific genotypes in starvation and deficient, whereas other hormone contents were similar. Association study showed that prolific was separated with non‐prolific ones at different doses of P. Finally, the prolific genotype is more adaptable with P deficiency.

Keywords

Abscisic acid; Acid phosphatase; Pti‐interacting serine/threonine kinase; Oil palm; P‐limitation

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DOI: https://doi.org/10.22146/ijbiotech.109941