Diversity of Nonribosomal Peptide Synthetase Genes in the AnticancerProducing Actinomycetes Isolated from Marine Sediment in Indonesia
https://doi.org/10.22146/ijbiotech.15266 Camelia Herdini(1*), Shinta Hartanto(2), Sofia Mubarika(3), Bambang Hariwiyanto(4), Nastiti Wijayanti(5), Akira Hosoyama(6), Atsushi Yamazoe(7), Hideaki Nojiri(8), Jaka Widada(9)
(1) Graduate School of Biotechnology, Universitas Gadjah Mada, Barek Utara, Yogyakarta, Indonesia ORL Head and Neck Department, Faculty of Medicine, Universitas Gadjah Mada, Sekip, Yogyakarta, Indonesia
(2) Graduate School of Biotechnology, Universitas Gadjah Mada, Barek Utara, Yogyakarta, Indonesia
(3) Graduate School of Biotechnology, Universitas Gadjah Mada, Barek Utara, Yogyakarta, Indonesia
(4) Graduate School of Biotechnology, Universitas Gadjah Mada, Barek Utara, Yogyakarta, Indonesia ORL Head and Neck Department, Faculty of Medicine, Universitas Gadjah Mada, Sekip, Yogyakarta, Indonesia
(5) Graduate School of Biotechnology, Universitas Gadjah Mada, Barek Utara, Yogyakarta, Indonesia
(6) Biological Resource Center, National Institute of Technology and Evaluation, Nishihara, Shibuya-ku, Tokyo, Japan
(7) Biological Resource Center, National Institute of Technology and Evaluation, Nishihara, Shibuya-ku, Tokyo, Japan
(8) Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo, Japan
(9) Department of Agricultural Microbiology, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Marine actinomycetes is a group of bacteria that is highly potential in producing novel bioactive
compound. It has unique characteristics and is different from other terrestrial ones. Extreme environmental
condition is suspected to lead marine actinomycetes produce different types of bioactive compound
found previously. The aim of this study was to explore the presence and diversity of NRPS genes in 14
anticancer-producing actinomycetes isolated from marine sediment in Indonesia. PCR amplification
and restriction fragment analysis of NRPS genes with HaeIII from 14 marine actinomycetes were done
to assess the diversity of NRPS genes. Genome mining of one species of marine actinomycetes (strain
GMY01) also was employed towards this goal. The result showed that NRPS gene sequence diversity in 14
marine actinomycetes could be divided into 4 groups based on NRPS gene restriction patterns. Analysis
of 16S rRNA gene sequences of representatives from each group showed that all isolates belong to genus
of Streptomyces. Genome mining result showed that strain GMY01 harboring 10 different NRPS gene
clusters that encode secondary metabolites, as pure NRPS or hybrid between NRPS and other compounds.
These results indicated that marine actinomycetes having a high potential to be developed as source of
anticancer drugs development.
compound. It has unique characteristics and is different from other terrestrial ones. Extreme environmental
condition is suspected to lead marine actinomycetes produce different types of bioactive compound
found previously. The aim of this study was to explore the presence and diversity of NRPS genes in 14
anticancer-producing actinomycetes isolated from marine sediment in Indonesia. PCR amplification
and restriction fragment analysis of NRPS genes with HaeIII from 14 marine actinomycetes were done
to assess the diversity of NRPS genes. Genome mining of one species of marine actinomycetes (strain
GMY01) also was employed towards this goal. The result showed that NRPS gene sequence diversity in 14
marine actinomycetes could be divided into 4 groups based on NRPS gene restriction patterns. Analysis
of 16S rRNA gene sequences of representatives from each group showed that all isolates belong to genus
of Streptomyces. Genome mining result showed that strain GMY01 harboring 10 different NRPS gene
clusters that encode secondary metabolites, as pure NRPS or hybrid between NRPS and other compounds.
These results indicated that marine actinomycetes having a high potential to be developed as source of
anticancer drugs development.
Keywords
marine actinomycetes; non-ribosomal peptide synthetase genes diversity; Streptomyces; genome mining; anticancer drugs development
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PDF DOI: https://doi.org/10.22146/ijbiotech.15266
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Copyright (c) 2016 Camelia Herdini, Shinta Hartanto, Sofia Mubarika, Bambang Hariwiyanto, Nastiti Wijayanti, Akira Hosoyama, Atsushi Yamazoe, Hideaki Nojiri, Jaka Widada
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