Selection of Yeast Strains for Ethanol Fermentation of Glucose-FructoseSucrose Mixture

J. Jasman(1*), Irfan Dwidya Prijambada(2), Chusnul Hidayat(3), Donny Widianto(4)

(1) Doctoral Program of Biotechnology, Graduate School of Universitas Gadjah Mada, Yogyakarta, Indonesia Department of Natural Sciences Education, Universitas Nusa Cendana, Kupang, Indonesia
(2) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author


This study was aimed to compare the ability of some yeast strains to consume sugars (sucrose, glucose
and fructose) and to convert them into ethanol during fermentation. The results of this comparison will be the
basis of considerations in choosing the right strain to be used as a mixed culture to increase the production
of ethanol from substrate containing a mixture of sucrose, glucose and fructose, such as juice of cane and
sweet sorghum. The study was conducted using fermentation in substrate consisting of glucose, fructose,
and sucrose separately, glucose-fructose mixture, and glucose-fructose-sucrose mixture using some yeast
strains: FNCC3012, OUT7009, OUT7027, OUT7055, OUT7080, OUT7096, OUT7903, OUT7913, and OUT7921.
Following the fermentation, analysis of the produced ethanol and the remaining sugar was conducted. The
results of study indicated that the strains with the highest substrate consumption were OUT7921, OUT7096,
OUT7055, OUT7027, and OUT7913 for glucose, fructose, glucose-fructose mixture, sucrose, and glucosefructose-sucrose mixture, respectively. Strains that produced highest concentration ethanol were OUT7096 in
glucose and sucrose substrates, OUT7921 in substrate of glucose-fructose mixture and sucrose, OUT7913 in
substrate of glucose-fructose-sucrose mixture. Upon consideration of each strain capacity, both in consuming
sugar and producing ethanol, the recommended strains for use in mixed culture in bioethanol fermentation
using mixed substrate of glucose, fructose and sucrose are OUT7096, OUT7913, and OUT7921.


Yeast; fermentation; mixed culture; sugar; ethanol


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