Molecular Identifcation of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product
https://doi.org/10.22146/ijbiotech.16368 Helen Joan Lawalata(1*), Langkah Sembiring(2), Endang Sutriswati Rahayu(3)
(1) Departement of Biology, Faculty of Mathematics and Natural Science, Manado State University, Manado, Indonesia
(2) Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Twenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditional
fermented fsh product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage
bacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. One
isolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilage
bacteria. This strain was further identifed by 16S rRNA gen sequence comparison. Isolates LAB producing
antimicrobial agents from bakasang were identifed as Pediococcus acidilactici.
fermented fsh product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage
bacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. One
isolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilage
bacteria. This strain was further identifed by 16S rRNA gen sequence comparison. Isolates LAB producing
antimicrobial agents from bakasang were identifed as Pediococcus acidilactici.
Keywords
Bakasang; LAB; antimicrobial; phenotypic characteristics; 16S rRNA gene
Full Text:
PDF DOI: https://doi.org/10.22146/ijbiotech.16368
Article Metrics
Abstract views : 2534
| 
views : 4963
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Helen Joan Lawalata, Langkah Sembiring, Endang Sutriswati Rahayu
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Past issues
The Indonesian Journal of Biotechnology (print ISSN 0853-8654; online ISSN 2089-2241) is published by the Research Center for Biotechnology in collaboration with the Graduate School of Universitas Gadjah Mada. The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License, and attributable to Siti Nurleily Marliana and Joaquim Baeta. Built on the Public Knowledge Project's OJS 2.4.8.1 and designed by Joaquim Baeta. View website statistics.