Genetic Variation of Apolipoprotein E (ApoE) in Surabaya, Palu and Alor Populations of Indonesia
https://doi.org/10.22146/ijbiotech.16371 Pramudji Hastuti(1*), Abdul Salam Mudzakir Sofro(2), Ahmad Husain Asdie(3), Ahmad Hamim Sadewa(4)
(1) Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Department of Biochemistry, Faculty of Medicine , YARSI University, Jakarta, Indonesia
(3) Department of Internal Medicine, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Apolipoprotein E (ApoE) has been considered to play an important role in cardiovascular disorders.
Several studies reported that genetic variation in ApoE locus influence plasma lipoprotein level. The objectives
of this study was to compare the frequency of ApoE genotypes and alleles in some populations of Indonesia.
One hundred and ninety five voluntarily unrelated apparently healthy individuals were recruited from
Surabaya, Palu and Alor representing the western, middle and eastern populations of Indonesia, respectively.
Blood samples were collected from each subject for DNA extraction. The common allelic variants of ApoE were
screened using polymerase chain reaction (PCR) and restriction fragment length polymorphism. Three alleles
i.e. ε2, ε3 and ε4 were identified and five genotypes i.e. ApoE ε2/ε2, ApoE ε2/ε3, ApoE ε3/ε3, ApoE ε2/ε4, ApoE ε3/
ε4 were found in three populations studied, while ApoE ε4/ε4 was absent in Surabaya, representing the western
populations of Indonesia. The frequency of ε2, ε3 and ε4 alleles in the western population were 0.208, 0.701
and 0.092 respectively; in the middle population were 0.242, 0.618 and 0.140 respectively and in the eastern
population of Indonesia were 0.267, 0.466 and 0.267 respectively. The highest frequency of ε2 and ε4 allele
was found in the eastern population of Indonesia. The distribution of ε2 allele were not significantly different
among all Indonesian populations, but significantly different were found in ε3 and ε4 allele in the eastern
population compared to those in the western and middle populations of Indonesian. It can be concluded that
the frequency of three ApoE alleles in the western and middle populations of Indonesia was not significantly
different however, significantly different was observed in the frequency of ApoE ε3 and ε4 alleles from the
eastern compared to those in the western and middle populations of Indonesia.
Several studies reported that genetic variation in ApoE locus influence plasma lipoprotein level. The objectives
of this study was to compare the frequency of ApoE genotypes and alleles in some populations of Indonesia.
One hundred and ninety five voluntarily unrelated apparently healthy individuals were recruited from
Surabaya, Palu and Alor representing the western, middle and eastern populations of Indonesia, respectively.
Blood samples were collected from each subject for DNA extraction. The common allelic variants of ApoE were
screened using polymerase chain reaction (PCR) and restriction fragment length polymorphism. Three alleles
i.e. ε2, ε3 and ε4 were identified and five genotypes i.e. ApoE ε2/ε2, ApoE ε2/ε3, ApoE ε3/ε3, ApoE ε2/ε4, ApoE ε3/
ε4 were found in three populations studied, while ApoE ε4/ε4 was absent in Surabaya, representing the western
populations of Indonesia. The frequency of ε2, ε3 and ε4 alleles in the western population were 0.208, 0.701
and 0.092 respectively; in the middle population were 0.242, 0.618 and 0.140 respectively and in the eastern
population of Indonesia were 0.267, 0.466 and 0.267 respectively. The highest frequency of ε2 and ε4 allele
was found in the eastern population of Indonesia. The distribution of ε2 allele were not significantly different
among all Indonesian populations, but significantly different were found in ε3 and ε4 allele in the eastern
population compared to those in the western and middle populations of Indonesian. It can be concluded that
the frequency of three ApoE alleles in the western and middle populations of Indonesia was not significantly
different however, significantly different was observed in the frequency of ApoE ε3 and ε4 alleles from the
eastern compared to those in the western and middle populations of Indonesia.
Keywords
Apolipoprotein E; genotypes; allele frequency; populations of Indonesia
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PDF DOI: https://doi.org/10.22146/ijbiotech.16371
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Copyright (c) 2016 Pramudji Hastuti, Abdul Salam Mudzakir Sofro, Ahmad Husain Asdie, Ahmad Hamim Sadewa
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