CYP3A4*1G gene Polymorphism on Javanese People

Em Sutrisna; Iwan Dwiprahasto; Erna Kristin

doi:10.22146/ijbiotech.16373

CYP3A4*1G gene Polymorphism on Javanese People

https://doi.org/10.22146/ijbiotech.16373

Em Sutrisna^(1*), Iwan Dwiprahasto⁽²⁾, Erna Kristin⁽³⁾

(1) Doctoral Program of Medical and Health Science, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia, Faculty of Pharmacy, Muhammadiyah University of Surakarta, Surakarta, Indonesia
(2) Department of Pharmacology and Toxicology, Faculty of Medicine Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Department of Pharmacology and Toxicology, Faculty of Medicine Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract

Most of drugs are metabolized by cytochrome P 450 (CYP) enzyme. Cytochrome P450 3A4 is the
cytochrome that is involved in metabolizing more than 60% of all medicine used in human. The variation
of this CYP3A4 gene will affect the catalytic activity of this enzyme. Recently, CYP3A4*1G in intron 10 was
found in Chinese and Japanese population. There is a substitution of G to A at position 82266 in intron 10. The
purpose of this research was to investigate the frequency of allele and genotype CYP3A4*1G. Samples were
taken from bloods of the subjects of the research. The examination of CYP3A4*1G was conducted by RTLP-PCR
method.As the results of this research, the frequency of CYP3A4*1G in Javanese people is CYP3A4*1/*1 0.25,
CYP3A4*1/*1G 0.55 and CYP3A4*1G/*1G 0.20. Frequency of allele G: 0.53, allele A: 0.47. The Fisher’s exact- test
shows that the allele and genotype frequencyis p. 1.000. The allele and genotype frequency of Javanese people
isstill in Hardy-Weinberg equilibrium.