Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium

https://doi.org/10.22146/ijbiotech.24195

Chusnul Hidayat(1*), Sari Darmasiwi(2), Maulina Nurikasari(3), Muhammad Nur Cahyanto(4),

(1) Department of Food and Agricultural Product Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta, Indonesia
(2) Department Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta
(3) Graduate School of Biotechnology, Universitas Gadjah Mada, Jl. Teknika Utara, Barek, Yogyakarta, Indonesia
(4) Department of Food and Agricultural Product Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Jatropha curcas seed cake contains a high amount of protein, and consequently has very high potential
as a medium for lipase production. The objective of this research was to characterize lipase from Aspergillus
niger 6516, which was produced by solid-state fermentation on Jatropha curcas seed cake as the medium. The effects of pH and temperature on enzyme activity were evaluated, along with substrate specifcity and enzyme stability. Fermentation was performed at a water concentration of 63% and temperature of 30 °C for 7 days. The results showed that the optimum pH and temperature for Aspergillus niger 6516 lipase activities were 8.0 and 40 °C, respectively. The lipase had the substrate specifcity to hydrolyze long-chain fatty acids and was stable in polar organic solvents. The lipase had a molecular weight, Km and vmax about 19 kDa, 0.27 µmol/ml, and 52.63 µmol/ml/min, respectively. The results also suggested that the produced lipase from Aspergillus niger 6516 was an alkaline lipase. Based on these results, we conclude that Jatropha seed cake is a suitable medium for lipase production.

Keywords


Lipase; Aspergillus niger; jatropha cake; solid state fermentation

Full Text:

PDF


References

Abigor, R.D., Uadia, P.O., Foglia, T.A., Haas, M.J., Scott, K. and Savary, B.J. 2002. Partial Purifcation and Properties of Lipase from Germinating Seeds of Jatropha curcas L. JAOCS., 79, 1123–1126.


Akanbi, T.O. and Barrow, C.J. 2015. Lipasecatalysed incorporation of EPA into emu oil: Formation and characterisation of new structured lipids. J. Funct. Foods, 19, 801 – 809.


Aguieiras, E.C.G., Cavalcanti-Oliveira, E.D. and Freire, D.M.G. 2015. Current status and new developments of biodiesel production using fungal lipase. Fuel 159: 52-67.


Alkan, H., Baysal, Z., Uyar, F. and Dogru, M. 2007. Production of lipase by a newly isolated bacillus coagulans under solidstate fermentation using melon wastes. Appl. Biochem. Biotechnol., 136,183-292.


Berchmans, H.J. and Hirata, S. 2008. Biodiesel production from crude Jatropha curcas L. seed oil with a high content of free fatty acids. Bioresour. Technol., 99, 1716–1721.

Bisen, P.S., Sanodiya, B.S., Thakur, G.S., Baghel, R.K. and Prasad, G.B.K.S. 2010. Biodiesel production with special emphasis on lipase-catalyzed transesterification. Biotechnol. Lett., 32, 1019–1030.


Bora, P.P., Bez, G. and Anal, J.M.H. 2011. First example of hydrolytic kinetic resolution of acrylate of secondary alcohols by lipase Amano AK. J. Mol. Catal. B-Enzym., 72, 270–275.


Bussamara, R., Fuentefria, A.M., de Oliveira, E.S., Broetto, L., Simcikova, M., Valente, P., Schrank, A. and Vainstein, M.H. 2010. Isolation of a lipase-secreting yeast for enzyme production in a pilot-plant scale batch fermentation. Bioresour. Technol., 101, 268–275.


Chahinian, H., Vanot, G., Ibrik, A., Rugani, N., Sarda, L. and Comeau, L.C. 2000. Production of extracellular lipase by Penicillum cyclopium purifcation and characterization of a partial acylglycerol
lipase. Biosci. Biotechnol. Biochem., 64 (2), 215-222.


Cihangir, N. and Sarikaya, E. 2004. Investigation of lipase production by a new isolate of Aspergillus sp. World J. Microbiol. Biotechnol., 20, 193–197.


Cho, H.Y., Bancerz, R., Ginalska, G., Leonowicz, A., Cho N. S. and Ohga, S. 2007. Culture conditions of psychrotrophic fungus Penicillium chrysogenum and its lipase characteristics. J. Fac. Agr., Kyushu Univ., 52 (2), 281–286.


Cho, S.H., Wang, P.Y. and Tsai, S.W. 2011. Lipase-catalyzed hydrolytic resolution of (R,S)-3-hydroxy-3-phenylpropionates in biphasic media. J. Taiwan Inst. Chem. Eng., 42, 408–412.

Chong, F.C., Tey, B.T., Dom, Z.M., Cheong, K.H., Satiawiharja, B., Ibrahim, M.N., Rahman, R.A., Biak, R.R.A. and Ling, T.C. 2007. Rice bran lipase catalyzed esterification of palm oil fatty acid distillate and glycerol inorganic solvent. Biotechnol. Bioprocess Eng., 12, 250-256.


Copeland, R.A. (2000). Enzymes: A practical introduction to structure, mechanism, and data analysis. New York: WileyVCH, Inc.

Damaso, M.C.T., Passianoto, M.A., Freitas, S.C., Freire, D.M.G., Lago, R.C.A. and Couri, S. 2008. Utilization of agroindustrial residues for lipase production by solid state-fermentation. Braz. J. Microbiol., 39, 676-681.


Ebongue, G.F.N., Dhouib, R., Carrière, F., Amvam Zollo, P.H. and Arondel, V. 2006. Assaying lipase activity from oil palm fruit (Elaeis guineensis Jacq.) mesocarp. Plant Physiol. Biochem., 44, 611–617.


Falony, G., Coca-Armas, J., Dustet-Mendoza, J.C. and Martinez-Hernández., J.L. 2006. Production of extracellular lipase from Aspergillus niger by solid-state fermentation. Food Technol. Biotechnol., 44 (2), 235–240.


Ferrer, M., Plou, F.J., Nuero, O.M., Reyes, F. and Ballesteros, A. 2000. Purifcation and properties of a lipase from Penicillium chrysogenum isolated from industrial wastes. J. Chem. Technol. Biotechnol., 75, 569 – 576.


Gombert, A.K., Pinto, A.L., Castilho, L.R. and Freire, D.M.G. 1999. Lipase production by Penicillium restrictum in solid state fermentation using babassu oil cake as substrate. Process Biochem., 35, 85–90.


Gunstone, F.D., Harwood, J.L. and Dijkstra, A.J. 2007. The lipid handbook. 3rd edition. New York: CRC Press Taylor and Francis Group.


Gutarra, M.L.E., Godoy, M.G., Maugeri, F., Rodrigues, M.I., Freire, D.M.G. and Castilho, L.R. 2009. Production of an acidic and thermostable lipase of the mesophilic fungus Penicillium simplicissimum
by solid-state fermentation. Bioresour. Technol., 100 (21), 5249-5524.


Hasan, F.A., Shah, A. and Hameed, A. 2006. Industrial Applications of Microbial Lipases. Enzyme Microb. Technol., 39, 235-251.


K a m i n i , N . R . , M a l a , J . G . S . a n d Puvanakrishnan, R. 1998. Lipase production from Aspergillus niger by solid-state fermentation using gingelly oil cake. Process Biochem., 33, 505–511.


Kempka, A.P., Lipke., N.L., Pinhero, T.L.F., Menoncin, S., Treicel, H., Freire, D.M.G., Di Luccio, M. and de Oliveira, D. 2008. Response surface method to optimize the production and characterization of lipase
from Penicillium verrucosum in solidstate fermentation. Bioprocess Biosyst. Eng., 31, 119–125.


Khan, N.R. and Rathod, V.K. 2015 Enzyme catalyzed synthesis of cosmetic esters and its intensifcational: A review. Process Biochem. 50: 1793-1806.


Kukreja, V. and Bera, M.B. 2005. Lipase from Pseudomonas aeruginosa MTCC 2488: partial purification and calcium dependent thermostability. Indian J. Biotechnol., 4, 222-226.


Mahapatra, P., Kumari, A., Garlapati, V.K., Banerjee, R. and Nag, A. 2011. Optimization of Process Variables for Lipase Biosynthesis from Rhizopus oligosporus NRRL 5905 Using Evolutionary Operation Factorial Design Technique. Indian J. Microbiol., 50 (4), 396-403.


Mahadik, N.D., Puntambekar, U.S., Bastawde, K.B., Khire, J.M. and Gokhale, D.V. 2002. Production of acidic lipase by Aspergillus niger in solid state fermentation. Process Biochem., 38 (5), 715–721.


Mahanta, N., Gupta, A., and Khare, S.K. 2008. Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate.
Bioresour. Technol., 99, 1729–1735. 


Maia, M.M.D., Morais, M. M.C., Morais, M.A., Melo, E.H.M. and Filho, J.L.L. 1999. Production of extracellular lipase by the phytophatogenic fungus Fusarium solani FS 1. Rev. Microbiol., 30, 304-309.


Marseno, D.W., Indrati, R. and Ohta, Y. 1998. A simplifed method for determination of free fatty acids for soluble and immobilized lipase assay. Indonesian Food Nutrition Progress, 5 (2), 79-83.


Mhetras, N.D., Bastawde, K.B. and Gokhale, D.V. 2009. Purification and characterization of acidic lipase from Aspergillus niger NCIM 1207. Bioresour. Technol., 100, 1486–1490


Pera, L.M., Romero, C.M., Baigori, M.D. and Castro, G.R. 2006. Catalytic properties of lipase extracts from Aspergillus niger. Food Technol. Biotechnol., 44 (2), 247–252.


Prazeres, J.N., Cruz, J. A. B. and Pastore, G.M. 2006. Characterization of alkaline lipase from Fusarium oxysporum and the effect of different surfactants and detergents on the enzyme activity. Braz. J. Microbiol., 37, 505-509.


Rifaat, H.M., El Mahalawy, A.A., El-Menofy, H.A. and Donia, S.A. 2010. Production, optimization and partial purification of lipase from Fusarium oxysporum. J. Appl. Sci. Env. Sanit., 5, 70-84.


Rodriguez, J.A., Mateos, J.C., Nungaray, J., Gonzalez, V., Bhagnagar, T., Roussos, S., Corvoda, J. and Baratti, J. 2006. Improving lipase production by nutrient source modifcation using Rhizopus homothallicus cultured in solid state fermentation. Process Biochem., 41, 2264–2269.


Shu, Z.Y., Yang, J.K. and Yan, Y.J. 2007. Purification and characterization of a lipase from Aspergillus niger F044. Chin. J. Biotechnol., 23(1), 96–100.


Silva, M.F., Freire, D.M.G., de Castro, A.M., DiLuccio, M., Mazutti, M.A., Oliveira, V., Treichel, H. and Oliveira, D. 2011. Production of multifunctional lipases by Penicillium verrucosum and Penicillium brevicompactum under solid state fermentation of babassu cake and castor meal. Bioprocess Biosyst. Eng., 34, 145–152.


Sun, S. and Xu, Y. 2009. Membrane-bound ‘synthetic lipase’ specifically cultured under solid-state fermentation and submerged fermentation by Rhizopus chinensis: a comparative investigation. Bioresour. Technol., 100, 1336–1342.


Toida, J., Arikawa, Y., Kondou, K., Fukuzawa, M. and Sekiguchi, J. 1998. Purifcation and characterization of triacylglycerol lipase from Aspergillus oryzae. Biosci. Biotechnol. Biochem., 62 (4), 759-763.


ul-Haq, I., Idrees, S. and Rajoka, M.I. 2002. Production of lipases by Rhizopus oligosporous by solid-state fermentation. Process Biochem., 37, 637–641.


Vokhlu, J. and Kour, A. 2006. Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electronic J. Biotechnol., 9 (1), DOI: 10.2225/vol9- issue1-fulltext-9.


Ward, O.P., Qin, W.M., Dhanjoon, J., Ye, J. and Singh, A., 2006. Physiology and biotechnology of Aspergillus. Adv. Appl. Microbiol., 58, 1–75.


Zang, K.P., Lai, J.Q., Huang, Z.L. and Yang, Z. 2011. Penicillium expansum lipasecatalyzed production of biodiesel in ionic liquid. Bioresour. Technol., 102, 2767–2772.



DOI: https://doi.org/10.22146/ijbiotech.24195

Article Metrics

Abstract views : 127 | views : 63

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Indonesian Journal of Biotechnology

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Indonesian Journal of Biotechnology indexed by:

ISSN 0853-8654 (Print)
ISSN 2089-2241 (Online)

 

Creative Commons License

The Indonesian Journal of Biotechnology, its website, and the articles published therein are licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. UGM logo © Universitas Gadjah Mada.

 

View Stats:

Details