Identifcation of antibiotic producing endophytic microbe isolates from a national park in Java island

https://doi.org/10.22146/ijbiotech.24345

Sri Yuwantiningsih(1*), Sebastian Margino(2), Subagus Wahyuono(3),

(1) Biotechnology, Study Program, The Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Endophytic microbes are potential sources of antibiotics. Some numbers of endophytic bacteria were isolated from plants in Ujung Kulon, Kaliurang, Meru Betiri and Baluran National Park, Bogor Botanical Garden, and Nusakambangan forest, Indonesia. Previous studies have been conducted to examine and obtain endophytic bacteria isolates from the selected plants, which resulted in three selected isolates, namely OOH-1, STG-1, and CMB-2. This research was conducted to determine the molecular identity of OOH-1 and STG-1 isolates, as well as to identify antibiotic compounds produced by STG-1 isolate. Molecular identifcation of selected isolates was based on 16S rRNA gene analysis and amplifed using primers 27F and 1492R. A phylogeny tree was then constructed by comparing the resulting sequences with data from Gene Bank using the BLAST-N program. The identifcation showed that STG-1 isolate had a 99% similarity with Pseudomonas brenneri strain SFML 97-391, and OOH-1 isolate had a 99% similarity with Enterobacter xiangfangensis. Identifcation of antibiotic compounds was done by purifcation and separation of the compounds. Antibiotic activity was also examined based on Lethal Concentration (LC50) on Fusarium oxysporum with a LC50 of 0.01–0.02% against Fusarium oxysporum.

Keywords


antibiotic; endophytic microbes; molecular identifcation

Full Text:

PDF


References

Bicchi, C.and Rubiolo, P. 1996. High performance liquid chromatographic particle mass spectrometri analysis of sesquiterpeneLacton with different carbon sceletons. Journal of Chromatography 727: 211-221.


El Sohly, H. N., Croom, E.M., El Feraly, F.S. and El Sherey M.M. 1990. A Large-scale extraction technique of artemisin from Artemesiaonnua. Journal of Nat. Prod. 53(6):1560-1565.


Joko T. and Kusumandari N. 2014. Deteksi molekuler bakteri penyebab penyakit busuk lunak pada anggrek menggunakan teknik Polymerase Chain Reaction. Pengembangan dan pemanfaatan IPTEKS
untuk kedaulatan pangan. Seminar Nasional Dies Natalis Faperta UGM ke-6


Jong de and Claessen, D. 2014, A sandwichculture technique for controlling antibiotik production and morphological development in Streptomyces coelicolor. Journal of Microbiological Methods. 10: 10-16.


He, R.I., Wang, G.P., Liu, X.H., Zhang, C.L and Lin.F.C. 2009. Antagonistic bioactivity of an endophytic bacterium isolated from Epimedium brevicornu Maxim African Journal of Biotecnology. 8(2): 191-195.


Lestiyani, A., Wibowo, A., Subandiyah, S. 2014. Uji metode inokulasi pada bawang merah dengan Fusarium spp. Pengembangan dan Pemanfaatan IPTEKS untuk Kedaulatan Pangan. Seminar Nasional Dies Natalis Faperta UGM ke-68.


Lockhart, S.L., Kiederma, D.J., Pfaller, M.A. 2009. The Epidemiology of Fungal Infection InAnaissie, EJ, McGinnis MR, Pfaller MA. (eds). Of Clinical Mycologv. 2nded oxford V K ElseviSp Inc. 


Margino, S. 2008. Produksi metabolit sekunder (antibiotik) oleh jamur endoft. Majalah Farmasi Indonesia 19: 86-94


Meliawati, R., Widyaningrum, D.N., Djohan, A.C., Sukiman, H. 2006. Pengkajian bakteri endofit penghasil senyawa bioaktif untuk proteksi tanaman. Jurnal Biodiversitas. 7(3) :221-224.


Muhammed, M., Fuchs, 13. B., Wu, M.P., Breger. J., Coleman, J.J., Mylonakis, E. 2012. The role of mycelium production and a MAPK-mediatedimune response in the C. elegans – Fusarium model system.
Journal of Medical Mycology. 50: 488-496.


Nasution, A., Usyati, N., and Santosa. 2014. Varietas lokal padi sebagai sumber ketahanan penyakit blas daun dan blas leher. Pengembangan dan pemanfaatan IPTEKS untuk kedaulatan pangan. Seminar Nasional Dies Natalis Faperta UGM ke-68.


Patel, H.A., Khristi, S.M., Perikh, K. and Rajeram G. 2012. Isolation and Characterization of Bacterial endophytic from Lycopersicumesculentum plant and their plant promoting characteristics. Nepal. Journal of Biotechnology 2(1): 37-52.


Rinanto, Y., Sumarni, T., Iskamto, B. 2008. Fraksi aktif jamur Candidaalbicans dari ekstrak metanalik bunga kamilen (Matricamachamommilla L.) Majalah Farrnasi Indonesia. 19 (2): 65-69.


Silverstein, R.M., Bassler, G.C. and Morrill, T.C. 1991. Spectometric identification organic compounds. Fifth Edition. John Willey and Sons Inc. Singapore 1-410.


Smitha, S.L. and Philip, R.K. 2014. Antibiotik Organic Compound production by a marine fungus Pencilliumcirinum S36 through solid state fermentation: optimization by response surface methodology. International Journal of Research Biomedicine and Biotechnology 4(1): 6-13.


Snook, M.E., Mitchel, T. Hinton D.M. and Bacon, C.W. 2009. Isolation and characterization of leu7-surfactin from the endophytic bacterium Bacillus mojavensis RRC 101, a biocontrol agent for Fusarium
verticillioides. Journal of Agriculture and Food Chemical. 57: 4287-4292.


Song, I., Ye.C., Zhang, Z., Lu, Y. and Ind Jing, K. 2014. Daptomycin antibiotic production processes in fed-batch fermentation by Streptomyces roseosporus NRRL. 11379 with precusor effect and medium optimization. Journal of Bioprocess Biosynthesis Enginering (37): 415-423


Strobel, G., Daisy, B., Castillo, U. and Harper J. 2004. Natural Product from Microorganism.Journal of Natural Product. 67: 257-269.


Tebbets, B., Yu, Z., Stewart, D., Zhao, L.X., Jiang, Y.I., Qu, L.H., Andes, D., Shen, B. and Klein, B. 2013. Identification of antitungal natural products via Saccharomyces cerevisiaebioassay : insight
in to macrotetrolide drug spektrum, potency and mode of action. Journal of Medical Mycolou. 51: 280-289.


Widiastuti, A. and Ningtyas, O.H. 2014. Identifkasi jenis jamur penyebab busuk pada beberapa buah pasca panen di Yogyakarta. Pengembangan dan pemanfaatan IPTEKS untuk kedaulatan pangan. Seminar Nasional Dies Natalis Faperta UGM ke-68.



DOI: https://doi.org/10.22146/ijbiotech.24345

Article Metrics

Abstract views : 114 | views : 66

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Indonesian Journal of Biotechnology

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Indonesian Journal of Biotechnology indexed by:

ISSN 0853-8654 (Print)
ISSN 2089-2241 (Online)

 

Creative Commons License

The Indonesian Journal of Biotechnology, its website, and the articles published therein are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. UGM logo © Universitas Gadjah Mada.

 

View Stats:

Details