Analyzing the biosynthetic potential of antimicrobial-producing actinobacteria originating from Indonesia

Anissa Utami; Pamela Apriliana; Yudi Kusnadi; Dewi S. Zilda; Zidny Ilmiah; Puspita Lisdiyanti; Siswa Setyahadi; Agustinus R. Uria

doi:10.22146/ijbiotech.65239

Analyzing the biosynthetic potential of antimicrobial-producing actinobacteria originating from Indonesia

https://doi.org/10.22146/ijbiotech.65239

Anissa Utami⁽¹⁾, Pamela Apriliana⁽²⁾, Yudi Kusnadi⁽³⁾, Dewi S. Zilda⁽⁴⁾, Zidny Ilmiah⁽⁵⁾, Puspita Lisdiyanti⁽⁶⁾, Siswa Setyahadi^(7*), Agustinus R. Uria⁽⁸⁾

(1) Graduate School of Pharmacy, Pancasila University, Jl. Raya Lenteng Agung, Jakarta Selatan 12630, Indonesia; Politeknik Hang Tuah Jakarta, Jl. Bendungan Hilir Jakarta Pusat 10210, Indonesia
(2) Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. LIPI, Cibinong, Bogor, Jawa Barat 16911, Indonesia
(3) Research Center for Marine and Fisheries Product processing and Biotechnology Ministry of Marine Affairs and Fisheries, JL. KS Tubun Petamburan VI, Slipi, Jakarta Pusat 10260, Indonesia; Life Science Division, ITS Science Indonesia, Jl. Boulevard Artha Gading, Jakarta Utara 14240, Indonesia
(4) Research Center for Marine and Fisheries Product processing and Biotechnology Ministry of Marine Affairs and Fisheries, JL. KS Tubun Petamburan VI, Slipi, Jakarta Pusat 10260, Indonesia
(5) Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. LIPI, Cibinong, Bogor, Jawa Barat 16911, Indonesia
(6) Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. LIPI, Cibinong, Bogor, Jawa Barat 16911, Indonesia
(7) Graduate School of Pharmacy, Pancasila University, Jl. Raya Lenteng Agung, Jakarta Selatan 12630, Indonesia; Center of Bio-industrial Technology, Agency for Technology Assessment and Development, Tangerang Selatan, Banten 15314, Indonesia
(8) Research Center for Marine and Fisheries Product processing and Biotechnology Ministry of Marine Affairs and Fisheries, JL. KS Tubun Petamburan VI, Slipi, Jakarta Pusat 10260, Indonesia; Faculty of Pharmaceutical Sciences, Hokkaido University, Kita Ward, Sapporo, Hokkaido 060-0812, Japan
(*) Corresponding Author

Abstract

We investigated the biosynthetic potential of soil-associated actinobacteria originating from Indonesia, identified as Streptomyces luridus and as Streptomyces luteosporeus. Antimicrobial assays indicated inhibitory activity by both strains against the pathogen Pseudomonas aeruginosa, with S. luteosporeus particularly inhibiting the growth of Bacillus subtilis. PCR-amplification, cloning, and sequencing of ketosynthase (KS) domains of type I modular polyketide (PKS-I) and adenylation (AD) domains of non-ribosomal peptide synthetase (NRPS) indicated the diversity of KS and AD domains derived from both Indonesian Streptomyces. Further phylogenetic analysis showed that KS domains from the subclass cis-AT PKS can be classified as being a part of a loading module or an extension module, along with their predicted substrate specificity. The results suggest that both strains are a potential source of novel biosynthetic pathways. This genetic analysis approach can be used as a fast guide to obtain insight into natural product biosynthetic gene diversity in microorganisms.

Keywords

Antimicrobial activity; biosynthetic potential; Streptomyces luridus; Streptomyces luteosporeus; PKS and NRPS gene diversity

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DOI: https://doi.org/10.22146/ijbiotech.65239