Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste
https://doi.org/10.22146/ijbiotech.8634 Sebastian Margino(1*), Erni Martani(2), Andriessa Prameswara(3)
(1)
(2)
(3)
(*) Corresponding Author
Abstract
Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate
(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB is
potential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. The
development of PHB production using starch as an alternative carbon source has been conducted to reduce
the dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processing
site were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of the
study showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB ranged
from 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 and
after optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubation
time. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed as
Micrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicated
as poly-β- hydroxybutyrate (PHB)
(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB is
potential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. The
development of PHB production using starch as an alternative carbon source has been conducted to reduce
the dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processing
site were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of the
study showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB ranged
from 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 and
after optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubation
time. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed as
Micrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicated
as poly-β- hydroxybutyrate (PHB)
Keywords
Poly-β-hydroxybutyrate (PHB); indigenous amylolytic bacteria; arrowroot starch
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PDF DOI: https://doi.org/10.22146/ijbiotech.8634
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