IDENTIFICATION OF CANDIDA YEAST IN STORED ISOLATES FROM SUBCKINICAL MASTITIS COW MILK

Popy Christin Ziliwu; Endang Yuni Setyowati; Wendry Setiyadi Putranto; Sarasati Windria

doi:10.22146/ijvs.v6i1.103635

IDENTIFICATION OF CANDIDA YEAST IN STORED ISOLATES FROM SUBCKINICAL MASTITIS COW MILK

https://doi.org/10.22146/ijvs.v6i1.103635

Popy Christin Ziliwu^(1*), Endang Yuni Setyowati⁽²⁾, Wendry Setiyadi Putranto⁽³⁾, Sarasati Windria⁽⁴⁾

(1)
(2)
(3)
(4)
(*) Corresponding Author

Abstract

Subclinical mastitis is a major issue in the dairy cattle industry, often caused by infections from microorganisms, including fungi of the genus Candida. This study aimed to identify Candida species in stored isolates from subclinical mastitis milk samples and analyze their characteristics. A total of 53 isolates derived from Stored Biological Materials (SBM) were tested using Sabouraud Dextrose Agar (SDA) and CHROMagar-Candida to identify species based on phenotypic characteristics and specific colony colors. The results showed that Candida tropicalis was predominant, accounting for 24 isolates (45.28%), followed by Candida krusei with 29 isolates (54.72%), while no isolates of Candida albicans or Candida glabrata were detected. These two Candida species are significant causative agents of subclinical mastitis, posing serious impacts on udder health, milk quality, and economic losses for farmers due to increased treatment costs.

Full Text:

PDF

References

Ahmad, R.Z. and Gholib, D. (2016). Mastitis Mikotik Akibat Terinfeksi Candida spp dan Trichosporon spp
pada Peternakan Sapi Perah di Bogor, Bandung, dan Jakarta Jurnal Veteriner. doi :
10.19087/jveteriner.2016.17.1.119.
Anggraeni, H.E., Nurfuadi, S.Z. (2021). Subclinical Mastitis Prevalence on Small Scale Dairy Farming in
Bogor. Journal of Applied Veterinary Science And Technology 2, 1. doi:
10.20473/javest.V2.I1.2021.1-4Badan Pusat Statistik. (2024). Jumlah Rata-Rata Konsumsi Susu tahun 2020. Jakarta (ID): Badan Pusat
Statistik.
Bayona, J. V. M., García, C. S., Palop, N. T., Martín, A. V., Padrón,C. G., Rodríguez, J. C., Pemán, J and
Cardona, C. G. (2022). Novel Chromogenic Medium CHROMagarTM Candida Plus for Detection
of Candida auris and Other Candida Species from Surveillance and Environmental Samples: A
Multicenter Study. doi:10.3390/jof8030281.
Du, J., Wang, X., Luo, H., Wang, Y., Liu, X., Zhou, X. (2018). Epidemiological investigation of non-
albicans Candida species recovered from mycotic mastitis of cows in Yinchuan, Ningxia of China.
BMC Vet Res 14, 251. doi:10.1186/s12917-018-1564-3.
Dolgun, H. T. Y., Kirkan, S., and Özavcı, V., (2022). Investigation of the Efficacy and Antifungal Drug
Resistance of Non-Albicans Candida Species in Mycotic Mastitis. doi : 10.31196/huvfd.1117714.
Gandhy, A., Kurniawati, S.D. (2018). Analisis Strategi Pengembangan Usaha Koperasi Produksi Susu
Bogor, Jawa Barat. Jurnal Maksipreneur: Manajemen, Koperasi, dan Entrepreneurship 8, 15.
doi:10.30588/jmp.v8i1.376.
Gomes, F., & Henriques, M., (2016). Control of Bovine Mastitis: Old and Recent Therapeutic
Approaches. Curr Microbiol, 72(4), pp.377-82. doi:10.1007/s00284-015-0958-8.
Jiang, C., Fang, W., Chen, S., and Guo, X. (2024). Genetic framework sequencing analysis of Candida
tropicalis in dairy cow mastitis and study of pathogenicity and drug resistance. MBC Microbiology.
doi: 10.1186/s12866-024-03522-y.
Milanov, D., Prunić, B., Velhner, M., & Bojkovski, J., (2014). Diagnosis of yeast mastitis in dairy cows.
MDPI. doi: 10.3390/agriculture14081292.
Nadeem, S.G., Hakim, S.T., Kazmi, S.U. 2010. Use of CHROMagar Candida for the presumptive
identification of Candida species directly from clinical specimens in resource-limited settings.
Libyan Journal of Medicine 5, 2144. doi : 10.3402/ljm.v5i0.2144.
Ningrum, I., Soeharsono, Wibawati, P., Dhamayanti, Y., Yunita, M., (2022). Faktor Risiko Kejadian Mastitis
Subklinis pada Sapi Perah di KPSP Ijen Makmur, Banyuwangi. Jurnal Medik Veteriner 5. doi:
10.20473/jmv.vol5.iss1.2022.48-53.
Nuryati, A., & Huwaina, A. D. (2015). Efektivitas Berbagai Konsentrasi Kacang Kedelai ( Glycine max (
L .) Merill ) Sebagai Media Alternatif Terhadap Pertumbuhan Jamur Candida albicans. Jurnal
Teknologi Laboratorium, 5(1), pp.5–8.
Prayoga, A., Bastian, and Aristoteles. (2023). Perbedaan Jumlah Koloni Jamur Candida albicans pada
Media Sabouraud Dextrose Agar (SDA) dan Media Modifikasi Biji Nangka (Artocarpus
heterophyllus lamk). JoIMedLabS 4(1), pp.78-86. doi: 10.53699/joimedlabs.v4i1.142.
Qurrahman, Muhammad Tuafiq & Rosid Wahyu Nugroho. (2015). Pengaruh Frekuensi Menguras
Terhadap Jumlah Candida sp. Pada Air Bak Toilet di SPBU Surakarta. Jurnal Ilmiah Biologi
Biogenesis.
Rahmanita, A., and Wahyuni, E., (2013). Isolasi dan Identifikasi Kapang dan Khamir dari Susu Sapi Perah
Normal, Mastitis Subklinis, dan Mastitis Klinis di Kabupaten Pacitan, Jawa TimurRosmania, & Yanti, F. 2020. Perhitungan JumLah Bakteri di Laboratorium Mikrobiologi menggunakan
Pengembangan Metode Spektrofotometri. Jurnal Penelitian Sains, 22(2), 76–86.
Wahyuningsih, and Pazra, D. F. 2022. Kualitas Fisik, Kimia, Mikrobiologi Susu Sapi pada Peternakan Sapi
Perah di Kecamatan Caringin, Kabupaten Bogor. Jurnal Agroekoteknologi dan Agribisnis, 6(1).
Yanuartono, Nururrozi, A., Indarjulianto,, S., Raharjo, S., Purnamaningsih, H., and Haribowo, N. 2019.
Review: mastitis mikotik pada ruminansia. Jurnal Ilmu-Ilmu Peternakan, 29(2), pp. 109 – 130. doi:
10.21776/ub.jiip.2019.029.02.03.