ACUTE TOXICITY TEST OF TABAR KEDAYAN ROOT EXTRACT (ARISTOLOCHIA FOVEOLATA MERR.) IN SWISS STRAIN FEMALE MICE (MUS MUSCULUS)

Abstract

The Tabar Kedayan (TK) is a plant known as an antidote for poison in the Dayak community. The part of the TK plant used widely for medication is the root. Empirically it is known to have benefits as an antidote, anti-diarrheal, anti-pain and anti-cancer. However, the toxicity of TK is unknown. This study had conducted to determine the toxic levels of TK root extract using the OECD 423 method. The toxicity test is a procedure used to determine the toxic levels of a chemical compound in the body through physical changes, behavior, and death of experimental animals. The experimental female mice used in the study had divided into two groups: the control group and the treatment group. Both groups contained three mice. The treatment group was given 300 mg/kg BW of TK extract orally, and the control group at 10 ml/Kg BW water. The treatment dose was then increased to 2000 mg/kg BW with new experimental animals because the treatment group did not show symptoms of acute intoxication at the first dose given. The experimental animals were observed for 14 days, and the results showed no sign of symptoms of acute intoxication, so the TK root extract was categorized as category 5, which was not classified, with an LD50 > 2000-5000 mg/kg BW.