The Anticoccidial Property of Sambiloto (Andrographis paniculata) Leaf Extract

Abstract

Andrographis paniculata, known as sambiloto, is a plant widely used in herbal medicine in the community. The most commonly used bitter plant is the leaves. Leaf-boiled water has a deep dark color and bitter taste. As an herbal plant, sambiloto has various benefits, such as antimalarial, antidiarrheal, antipyretic, and anti-inflammatory properties. Andrographolide is a typical compound found in Sambiloto extract. In addition, other compounds can be found in the quote, namely flavonoids, alkaloids, tannins, saponins, and steroids. Several studies have discussed the potential of these five compounds to overcome coccidial infections in poultry. Eimeria tenella infection in poultry occurs quickly in the field, and its impact is detrimental. Chickens become stunted and unhealthy. Therefore, this study aimed to determine the potential of sambiloto extract with water and 80% ethanol against Eimeria tenella infection. In addition, this study also aimed to determine the phytochemical content of Sambiloto extract in water and 80% ethanol. Andrographolide was detected using UPLC. A UV-vis spectrophotometer was used to test for flavonoids, tannins, alkaloids, saponins, and phenols, and densitometry TLC was used to test the total steroids in the extracts.