Evaluation of Protein Protected in the Cow Beef Cattle Rations Base-on the Fermentation and Microbia Activities Ruments by In Vitro

Abstract

The purpose of this study was to evaluat the effect of protein protection in the rations of  beef cattle on rumen fermentation and microbial activities in vitro. Soybean groats as a source of protein. Protection using 37% formaldehyde at 2% dry matter of feed mixture of soybean groats and lemuru fish oil was conducted. Rumen fluid were taken from the Ongole crossbred cow fistulated. Rumen fermentation was observed 48h of incubation in rumen fluid by in vitro. Feed treatments including T0 (30% fermented straw+30% elephant grass+40%  control concentrate), T1 (30% fermented straw+30% elephant grass+32.5% control concentrate+7,5% protein unprotected), and T2 (30% fermented straw+30% elephant grass+32.5%  control concentrate+7,5% protein protected). The results of the rumen fermentation by in vitro evaluation showed that pH, ammonia and VFA levels were not significantly affected by the treatment.  The microbial activity by in vitro evaluation  showed that microbial protein synthesis and CMC-ase were not significantly differenrt (p>0.05) but protozoa population significantly reduced (p<0.05). The number of protozoa in the 7.5% of protected proteins in the cow beef cattle rations was lower than in other treatments in vitro. It was concluded that are rumen fermentation and rumen microbial activity by in vitro evaluation were not affected by the formaldehyde treatment. Formaldehyde treatmentat 7.5%  resulted in lower number of protozoa but this did not interfere with the continuity of in vitro rumen fermentation.